M. REBBAS Khellaf

The German cockroach (Blattella germanica) is a resilient pest that exhibits a high reproductive
capacity, posing significant risks to hygiene and human health. Recently, plant extracts have emerged
as promising, environmentally safe alternatives for insect control. To mitigate the harmful effects of
Blattella germanica, we conducted experiments using Ruta chalepensis. Preliminary trials involved
administering aqueous plant extracts by ingestion, testing varying concentrations and exposure times to
establish a sub-lethal dose (500 µg/mL). This dose was selected to study the plant’s effects on the pest’s
behavior. Sub-lethal concentration (500 µg/mL) of the extract disrupted sexual attractiveness. Results
confirmed that 86.66% of untreated control adults were attracted to extracts from untreated males and
females. In contrast, adults treated with R. chalepensis leaf extracts (500 µg/mL) showed significantly
reduced attraction (20–30%) to extracts from both treated and untreated individuals, regardless of sex.
Additionally, sub-lethal concentrations altered the progression of sexual behavior phases. Treated
partners exhibited no successful mating events, whereas untreated controls achieved an 80% success
rate. These findings suggest that sub-lethal doses of this bio-insecticide interfere with chemical signals
critical for mate recognition, impairing the cockroaches’ ability to detect or interpret these cues.

Etude de l’effet de larvicide de l’extrait aqueux des feuilles d’Euphorbia flavicoma sur les blattes urbaines (Blattella germanica).

Salsola kali L., a halophytic plant renowned for its resilience in arid and saline environments, possesses a rich and diverse
phytochemical pro2le with signi2cant bioactive potential. .is study provides a comprehensive phytochemical and pharmacological evaluation of S. kali extracts, employing an integrative analytical approach combining gas chromatography-mass
spectrometry (GC-MS), inductively coupled plasma-optical emission spectrometry (ICP-OES), and high-performance liquid
chromatography-diode array detection (HPLC-DAD). .e phenolic composition of the methanolic extract revealed 17 identi2ed
compounds, with quercetin 3-O-β-galactoside (14.74 mg/g extract) and pyrocatechol (14.54 mg/g extract) being the most
abundant, followed by other notable compounds highlighting the plant’s phenolic diversity. Furthermore, bioavailability and
pharmacokinetic properties of the identi2ed bioactive compounds were predicted using SwissADME and pkCSM tools, revealing
favorable drug-like characteristics. GC-FID analysis revealed that the hexane extract primarily contained saturated fatty acids,
with palmitic acid being the most abundant, comprising 55.17% of total fatty acids. .e mineral content analysis revealed a rich
pro2le of essential macro- and micronutrients, with potassium (2.62%) as major constituents, alongside high levels of iron
(379.82 mg/L). Pharmacological assessments demonstrated signi2cant antioxidant activity, with the methanol extract showing
moderate DPPH radical scavenging activity (IC50 � 90.85±0.91μg/mL), while the hexane extract exhibited lower activity
(IC50 � 542.59±2.68μg/mL). .e methanol extract also displayed promising enzyme inhibitory eEects, with moderate activity
against butyrylcholinesterase (BChE) and notable inhibition of α-glucosidase. However, acetylcholinesterase (AChE) inhibition
was not observed at concentrations below 200μg/mL. .is study elucidates the structural and functional attributes of S. kali
metabolites, emphasizing their potential as antioxidants, enzyme inhibitors, and nutrient sources for therapeutic use.

Effet toxique de deux solanacées ( Nicotiana glauca et Solanum nigrum) contre la blatte germanique

Abstract
The Salvia genus, one of the largest in the Lamiaceae family, is present in North Africa with 27 different taxa. In this study, the chemical composition of Salvia lanigera and Salvia taraxacifolia essential oils (EOs) was studied. The latter species has never been investigated. The two EOs, extracted by hydro-distillation and analysed by GC-MS, were rich in terpenoids. Monoterpene hydrocarbons were the major chemical group of S. lanigera (88.0%) (12), while S. taraxacifolia EO (18), consisted of monoterpene hydrocarbons (35.7%), with limonene (24.9%) as the most abundant compound, while β-cubebene (22.7%) was identified as primary hydrocarbon sesquiterpene compound (38.7%). Furthermore, statistical analyses such as hierarchical cluster analysis (HCA) and principal component analysis (PCA), employed to highlight similarities or dissimilarities between Salvia taxa, showed clear divergences between chemical and botanical classification.

The phytochemical profile of the aqueous methanolic extract from the aerial parts of Psychin
stylosa Desf., an Algerian endemic species belonging to the Brassicaceae family and previously
unstudied, was analysed using the HPLC-MS/MS technique, allowing for the identification of 65
compounds from diverse chemical classes, including phenolic compounds, flavonoids, sugars, alkaloids,
sulphur compounds, peptides, terpenoids, and steroids, as well as 13 unidentified compounds. The
extract showed a high total polyphenol content of 64.39 ± 1.63 mg GAE/g extract and a flavonoid
content of 20.39 ± 0.53 mg QE/g extract, indicating its richness in bioactive phenolic constituents. It
also exhibited strong antioxidant activity, with an IC50 value of 9.82 ± 1.70 µg/mL in the DPPH assay,
while no antibacterial effect was observed against the tested bacterial strains. This study represents
the first report combining phytochemical characterisation and biological evaluation of this plant
species, highlighting its potential as a natural source of antioxidant compounds.

First Inventory and Taxonomic Checklist of Forest Cockroaches in Bouira region (Northern Algeria), study of their natural habitat

Chemical Composition and QSRR Analysis of Essential Oil of Algerian Scabiosa Daucoïdes Desf

This investigation evaluated the chemical composition and the biological activities of the ethanol and petroleum ether extracts of Salvia lanigera Poiret from the M’sila region, Algeria. Phytochemical analysis identified 17 compounds in the ethanol extract (HPLC–DAD), with cynarin, ellagic acid, and rutin as major components. Petroleum ether extract (GC–MS) revealed 16 compounds, predominantly palmitic acid and stearic acid. Antioxidant activity was assessed using four assays: the ethanol extract showed significant activity in the phenanthroline assay (1.94 ± 0.18 μg/mL), and SNP assay (124.78 ± 0.59 μg/mL), compared to the BHA standard. Both extracts demonstrated antibacterial and antifungal effects, with inhibition zones of 10–13 mm and MIC values ranging from 0.78 to 3.125 mg/mL against tested strains. Enzymatic assays revealed α-glucosidase inhibition by the ethanol extract (IC50 = 27.07 ± 0.78 μg/mL), while α-amylase inhibition was lower (ethanol: 429.85 ± 1.43 μg/mL; petroleum ether: 520.31 ± 1.63 μg/mL). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition were minimal (IC50 > 200 μg/mL for AChE; ethanol: 365.84 ± 5.48 μg/mL, petroleum ether: 636.13 ± 4.49 μg/mL for BChE). Urease inhibition was notable for the ethanol extract (54.88%) and comparable for the petroleum ether extract (52.00%). These findings highlight the potential of S. lanigera extracts as sources of bioactive compounds with antioxidant, antimicrobial, and enzymatic inhibitory properties, warranting further exploration for therapeutic applications.

In this paper, the first phytochemical analysis on Teucrium chamaedrys subsp. gracile was reported. The dried aerial parts collected in Algeria were studied, and the analysis was conducted using different techniques: gas chromatography/mass spectrometry (GC/MS) for the fatty acid content, headspace solid-phase microextraction-GC-MS for the volatile composition, column chromatography along with nuclear magnetic resonance spectroscopy, and MS for non-volatile pattern characterization. Saturated fatty acids were found to be prevalent, with palmitic acid as the major compound. In the volatile fraction, β-caryophyllene and humulene, comprised in the class of sesquiterpenes, were the major components. Indeed, five non-volatile compounds were observed, namely teucrioside (1), cirsiliol (2), harpagide (3), and chlorogenic acid (4). The employed analytical techniques have proven to be suitable for producing reliable and reproducible results in the description of the phytochemical profile of the matrix.

The genus Onobrychis Miller, belonging to the Fabaceae family, has wide distribution ranging from
Europe to Mongolia and Himalaya, Arabian Peninsula, Ethiopia, with eastern Mediterranean area and
western Asia considered as the centres of diversity. Plants from this genus have been used for centuries
around the world, as forage for ruminant animals, both for grazing and as hay. The use of some species
in traditional medicine has been also documented. Onobrychis kabylica (Bornm.) Širj. is a perennial
species, that primarily thrives in temperate biomes endemic of Morocco, Algeria, and Tunisia. In this
study, the chemical profile of the essential oil extracted from the aerial parts (Ok), never been
previously analysed, was assessed using GC-MS. The Ok was found to be rich in oxygenated
monoterpenes (44.4%) comprising of verbenone (14.0%) and borneol (10.4%) as main compounds.

This study sought to maximize the extraction of Glebionis coronaria’s total phenolic content (TPC) and total flavonoid content (TFC), determining the best conditions for separating these beneficial substances. Liquid–liquid partitioning was carried
out after extraction using solvents of different polarities, such as n-butanol, ethyl acetate, and chloroform. While antioxidant
activity was evaluated using DPPH, ABTS, reducing power, phenanthroline, and silver nanoparticle assays, TPC and TFC
were measured using the Folin-Ciocalteu and AlCl₃ techniques, respectively. Cytotoxic effects were evaluated against two
cancer cell lines (CAPAN-1 and dld-1) as well as a healthy cell line (L929), and the ethanolic extract’s substance composition
was examined using LC–ESI–MS/MS. The optimal TPC and TFC extraction parameters were found to be X1 (48 h), X2 (70%
ethanol), and X3 (30 mL/g). The n-butanol fraction showed significant antioxidant activity (IC50: 13.89 μg/mL for DPPH,
29.18 μg/mL for ABTS) and had the highest TPC (325.33 mg GAE/g dw) and TFC (112.5 mg QE/g dw). Additionally, it
demonstrated a considerable ability to chelate metals. Twelve bioactive compounds were identified in the ethanolic extract,
with chlorogenic acid being the main component (422.47 μg/g). Cytotoxicity testing revealed significant effects against
CAPAN-1 cells, reducing viability by 38.53% at 1 mg/mL. An ADME model provided insights into the pharmacokinetics
and bioavailability of the identified compounds, highlighting their therapeutic applications.

Modérateur du séminaire

Membre du comité scientifique

This study comprehensively analyzed the phytochemical profile, antioxidant activity, and enzyme-inhibitory properties of
Rumex vesicarius (R. vesicarius) extracts using advanced food analytical methods. The methanol extract was profiled using
high-performance liquid chromatography with diode array detector (HPLC–DAD), identifying 16 phenolic compounds, with
cynarin (26.06 mg/g dry weight extract) as the most abundant compound, revealed for the first time in this species. The hexane
extract was assessed by gas chromatography-mass spectrometry (GC–MS), revealing 12 compounds, predominantly linoleic
acid (46.09%). Mineral content was measured using inductively coupled plasma mass spectrometry (ICP-MS), showing high
concentrations of iron (497.03 mg/L) and calcium (3.31%). The methanol extract exhibited strong antioxidant activity, with
IC 50 values of 47.25 µg/mL (DPPH assay) and 26.98 µg/mL (ABTS assay) and an A0.5 value of 33.76 µg/mL in the reducing
power assay. The hexane extract exhibited significant enzyme-inhibitory activity against α-amylase (IC50=33.80 µg/mL) and
α-glucosidase (IC50 = 154.19 µg/mL). Additionally, ADME/T (absorption, distribution, metabolism, excretion, and toxicity)
analysis of the HPLC–DAD-detected compounds indicated favorable drug-likeness and pharmacokinetic properties. These
findings underline the promise of R. vesicarius as a rich source of bioactive compounds with antioxidant and antidiabetic
properties, making it a promising candidate for further research in functional foods. The use of robust analytical methods,
including HPLC–DAD, GC–MS, and ICP-MS, ensures reliable and reproducible results, aligning with the standards of food
analytical methodologies.

This study explores the potential of Thymus ciliatus as a natural source of bioactive compounds by investigating its chemical composition and in vitro biological activities, including antioxidant, antimicrobial, antidiabetic, and anti-Alzheimer properties. The analysis of the essential oil was performed using GC-MS revealing α-terpinenyl acetate (18.74%) and camphor (10.62%) as the major components. Antioxidant activity was evaluated using six methods. Antimicrobial activity was assessed using the disc diffusion and well diffusion techniques. Antidiabetic activity was measured through a colorimetric assay, while anti-Alzheimer activity was evaluated against butyrylcholinesterase (BChE). The results demonstrated that extracts from polar and medium-polar solvents exhibited the highest antioxidant activity, followed by low-polar solvent extracts. The essential oil of T. ciliatus displayed significant antimicrobial activity particularly against Staphylococcus aureus, Bacillus cereus, and Candida albicans. Crude extracts also showed antimicrobial activity across all tested strains. The aqueous extract exhibited the strongest antidiabetic activity against α-glucosidase (IC50 = 2.56 ± 0.06 µg/mL), followed by the essential oil (IC50 = 57.11 ± 4.39 µg/mL). Furthermore, the dichloromethane extract demonstrated the highest anti-Alzheimer activity with an IC50 of 0.26 ± 0.20 µg/mL. Based on these results, T. ciliatus represents a promising source of bioactive substances with potential therapeutic applications.

La Grande Kabylie est un point chaud régional caractérisé par une multitude
d’écosystèmes, depuis le littoral jusqu’aux hautes montagnes. La seule étude de référence sur les orchidées
n’a pas pu prendre en considération tout le territoire de la wilaya de Tizi Ouzou, contrairement à la wilaya
voisine de Béjaïa. Le présent travail vient compléter l’inventaire, l’écologie et la répartition des différentes
espèces d’orchidées dans la wilaya. Après une année de prospections, un bilan de 45 taxons autonomes plus
5 hybrides occasionnels répartis en 11 genres (Anacamptis, Androrchis, Cephalanthera, Epipactis,
Dactylorhiza, Limodorum, Neotinea, Ophrys, Orchis, Serapias, Spiranthes) a été noté au niveau de 275
stations distinctes en recueillant plus de 1000 observations uniques. Cet inventaire fait de la wilaya la plus
riche à ce jour pour l’orchidoflore algérienne. Ces espèces recensées ont donc une grande valeur en termes
d’enjeux de conservation, soit pour des raisons patrimoniales (endémiques, protégées), soit pour leur risque
d’extinction (menacées, rares).
Mots-clés. – Cartographie de la biodiversité, espèces endémiques, espèces menacées, espèces
protégées, espèces rares, priorités de conservation, responsabilité patrimoniale.

• In 2009, PlantLife, the IUCN Mediterranean Center for Cooperation and the WWF initiated the process for identifying Important
Plant Areas (IPAs) in northern Algeria, including capacity building, data review, and help for publication. The first step was the
edition of a first IUCN report on “Important plant areas of the south and east Mediterranean region” in 2011. At this stage, 21
IPAs had been identified in northern Algeria, and the process more detailed in an international publication by Yahi et al. in 2012,
adding a 22nd at the same time.
• Then, a national dynamic started and few new IPAs were added opportunely, until a new editing process supported by IUCN and
the MAVA foundation that permitted the publication of a second report in 2018 on “Conserving wild plants in the south and east
Mediterranean region”. At this stage, northern Algeria totalized 39 Key Biodiversity Areas for plants (i.e. IPAs).
• At national level, Algerian botanists pursued their field inventories and the identification process of new IPAs. At end of 2024
more than 40 IPAs are identified and several others are already on their way thanks to targeted field studies, PhD thesis and
their corroborated scientific publications through diverse areas of the country.
• From a methodological point of view, three generations of Algerian IPAs are parallelly developed and progressively incremented.
The first generation was created based on historical bibliography, aiming to generate a first list of trigger species. The second
generation was based on frugal field surveys aiming to rediscover and confirm the occurrence of trigger species. The third
generation, now beginning, both aims to redefining the trigger species list based on a revised taxonomy and to redelimiting the
IPA perimeters based on a concrete terrain mapping. These new ambitions face various difficulties which will have to be
addressed and gaps which will have to be filled.
• From a conservational point of view, NGOs and administrative services are gradually taking ownership of this informative but
non-binding classification and are using it to support their requests for the classification of natural sites and the creation of
protected areas.
• Keywords : biodiversity mapping, capacity building, decision support, field surveys, in situ conservation, taxonomical shortfalls

This study represents the first investigation of the volatile composition of Calystegia silvatica (Convolvulaceae) collected in Algeria, using headspace solid-phase microextraction and gas chromatography/mass spectrometry (HS-SPME-GC/MS). This technique allowed the identification of 44 compounds, constituting 96.9% of the total components. These compounds belong to various chemical classes, including sesquiterpene hydrocarbons (22) and non-terpene derivatives (13), as major groups. Remarkably, the volatile profile of C. silvatica was dominated by sesquiterpenoids (87.1%), particularly sesquiterpene hydrocarbons (83%). Analysis of the aroma profile revealed that the main metabolites were β-caryophyllene, α-gurjunene and germacrene D. Oxygenated sesquiterpenes were present in a smaller proportion (4.1%), mostly represented by palustrol. Other chemical classes, such as monoterpene hydrocarbons, oxygenated monoterpenes, non-terpene derivatives and apocarotenes, have been found in limited quantities. It is worth noting that the data presented in this study have not previously been reported for Calystegia species.

Desertification represents a pressing global concern, with Algeria facing significant challenges in
this regard. The Hodna region is undergoing rapid landscape transformation due to
Desertification. Protecting against desertification and improving the living conditions of
dependent populations have become national priorities in Algeria. The aim of this study is to
assess desertification sensitivity, develop a set of thematic maps (including vegetation quality,
soil quality, etc.), and established GIS-based of the study area using Geographic Information
System (GIS) based on the MEDALUS (Mediterranean Desertification and Land Use) approach.
The Desertification Sensitivity Index (DSI) is derived by integrating four primary indicators:
Soil Quality Index (SQI), Climate Quality Index (CQI), Vegetation Quality Index (VQI), and
Anthropogenic Quality Index (AQI). The analysis of the desertification sensitivity map for the
Boussaada sub-basin identified five distinct zones: highly sensitive, sensitive, moderately
sensitive, less sensitive, and non-sensitive. The findings reveal that 76% of the Boussaada area is
categorized as sensitive to highly sensitive to desertification. This region is defined as a predesert zone,
characterized by an arid climate with scarce and erratic rainfall, alongside
inadequate land management practices

During routine inventories of the flora of Kabylia, we were able to make the
regional rediscovery in Aït Ziki (wilaya of Tizi-Ouzou) of Squilla anthericoides
(syn. Charybdis anthericoides, Drimia anthericoides, Scilla anthericoides, Urginea
anthericoides, Urginea maritima var. anthericoides), an endemic to northeastern
Algeria that had not been seen in Kabylia since the end of the 19th century. This
poorly known species was rehabilitated in 2016 only, which is why we provide
here a complementary historical and ecological assessment for this autumn
flowering geophyte listed on the IUCN Red List.

Cachrys sicula L. collected in Algeria: the chemical composition of the essential oils by GCMS analysis

In-Depth Chemical Profile by GC–MS, ICP-OES and HPLC–DAD, and In Vitro Antioxidant Properties of Lavandula multifida

First entomological inventory of Forest Cockroaches of Bouira Region (Northern Algeria)

Chemical profiling by HPLC-DAD and GC-MS analysis and antimicrobial potential of Achillea santolina plant extracts against ESBL- producing E. coli and fungal pathogens

Valorisation of Algerian medicinal plants : exploring polyphenol content and antioxidant activities.

Direct and delayed effect of the plant Cleome amblyocarpa Barratte & Murb (Capparidaceae) on the two species of (Blattodea) Blattella germanica (Linnaeus, 1767) and Shelfordel lateralis (Walke1868).

The allergenic pollen flora of the Maadid region includes 59 species belonging to 19 botanical
families and 44 genera and is represented by 47 anemophilous species and 12 entomophilous species
responsible for allergies. This flora was inventoried during March to April 2019. Three families,
Poaceae, Anacardiaceae and Plantaginaceae dominate respectively. The chorological types of the
species show the dominance of the elements of the Mediterranean group over all the other groups,
followed by the cosmopolitan group. The biological types of species reveal the dominance of
phanerophytes and therophytes over all biological types, hemicryptophytes and geophytes.

On current distribution of Androctonus aeneas Koch, 1839 in Algeria (Scorpiones: Buthidae).

Catalogue des plantes vasculaires du Djebel Ouarsenis (Tissemsilt, Algérie)

Botanical surveys conducted in the spring of 2024 in the Setif province in northeastern
Algeria led to the rediscovery of Ophrys omegaifera subsp. hayekii (H. Fleischm. & Soó)
Kreutz and Orchis purpurea Huds. in the Harbil region. Ecological assessments of these
species are also provided. This rediscovery enriches the Algerian orchid flora, particularly
in the Setif region.

Cistus libanotis from Algeria: Phytochemical, Analysis by GC/MS, HS-SPME-GC/MS, LCMS/MS and its anticancer activity

La composition chimique des huiles essentielles des parties aériennes de Limonium lobatum croissant à l’état sauvage en Algérie.

Phytochemical Profiling, LC-MS Analyses, Cytotoxicity, Antioxidant, and Antimicrobial Activities of Ruta montana : An Ethnomedicinally Important Plant in Algeria.

This study is topical and is of particular importance with the emergence and spread of vectorborne diseases in urban areas around the world, and in Algeria in particular. In this work we carried
out a toxicological test on the larvae of two species namely Culiseta longiareolata and Blattella
germanica which are considered harmful and can be carriers of many pathogens, with the use of the
aqueous extract of the plant Euphorbia bupleuroides for a certain time, the results of toxicity give a
toxic effect on the larvae determined at different lethal concentrations and sublethal (LC 50 % and LC
90 %).

During the current study, the phenolic and flavonoid contents were measured in Physalis acutifolia extracts with Folin–Ciocalteu and AlCl3 methods, respectively. Various extract was assessed on cancer cell lines (i.e., CAPAN-1 and dld-1) and a healthy cell line (i.e., L929). Optimal parameters for polyphenol extraction were determined: extraction time of 72 h, solvent-to-plant ratio of 10 mL/1 g, and plant concentration of 90%. The n-butanol extract showed the highest phenolic and flavonoid contents (i.e., 263.84 mg GAE/g dw and 72.03 QE/g dw, respectively), along with superior antioxidant and anti-enzymatic activities with IC50 values of 49.77 and 187.12 μg/mL with acetylcholinesterase and butyrylcholinesterase assays, respectively. LC-ESI-MS/MS analysis revealed 12 components, with hesperidin being the most abundant (i.e., 1829.0001 μg/g). The ethanolic extract exhibited cytotoxic effects on cancer cell lines, with an IC50 value of 0.959 mg/mL for dld-1, but with no effect on healthy cells. The bioavailability scores of the identified components support the in vitro findings and confirm the different assessed
therapeutic effects.

Activité biologique de Lavandula officinalis de la région de Msila.

Valorisation of the biological activities of Solanum elaeagnifolium and Eryngium campestre.

Phytochemical study & Biological potency of the medicinal plant «Bassia indica»

The aim of the study is to evaluate the antigerminative effects of the essential oil from Elaeoselinum fontanesii Boiss. (Apiaceae) grow spontaneously in Algeria, against the germination and the radicle growth of seeds from cultivated plant Triticum durum Desf.
The volatile oil obtained by hydrodistillation from the aerial parts of E. fontanesii was analyzed by gas chromatography–mass spectrometry. 41 constituents were identified, representing 92% of the total oil, monoterpenes was the major chemical groups (75.9%) and high content of myrcene that was a characteristic compounds. The germination and radicle growth of seeds of durum wheat were controlled in different concentrations of the plant extract. The results showed high inhibition against the growth of radicale and the germination of the seeds.

The Limonium genus (Plumbaginaceae) includes several species of perennial herbs and shrubs belonging to a particular type of halophytes, known as ‘recretohalophytes’. Limonium species are widely distributed in the Mediterranean region, mainly in the North-Eastern and Southern countries and several bioactivities have been well documented. In the present study, the chemical composition of the essential oil (EO) of the aerial parts of Limonium lobatum (L.f.) Chaz., a species never chemically previously studied and collected in Algeria, which grows in South Spain, North Africa, and SW Asia, was analysed using GC-MS. The main constituents of the EO were monoterpenes involving eucalyptol (14.21%), β-pinene (8.62%), β-myrcene (8.18%). Among the sesquiterpene compounds β-caryophyllene (8.94%) was the major one. The chemical profile of the EO presented here was compared with the EOs of previously investigated Limonium taxa. Furthermore, a complete literature review on the ethno-pharmacological uses of Limonium species was performed.

Following the publication of the article on the synthaxonomic study of the Dreat forest, the
aim of this inventory of the Dreat forest’s flora (semi-arid zone in central Algeria) to allow us
a quantitative analysis on the basis of biogeographic parameters such as specific richness,
geography of distribution, endemism and rarity. A subjective sampling was carried out
between 2018 and 2021 at the level of the various formations (forest, matorral, altitude
grasslands, and steppe). This analysis showed considerable species richness, estimated at
around 468 taxa belonging to 278 genera and 66 families. Therophytes dominate the other
living forms, according to the defined biological spectrum. From a biogeographical point of
view, Mediterranean species constitute more than 50 percent of the study area's species,
followed by endemic species and rare.

Mosquitoes have always been considered a source of harm to humans and animals mainly
because they can be vectors of disease. This work represents a study on the effect of aqueous
extract of three plants (Hyoscyamus albus, Solanum elaeagnifolium, Solanum nigrum) of the
Solanaceae family on the mortality of 4th stage larvae of Culiseta longiareolata larvae.
Extract of the high impact is of S. nigrum followed by H. albus and S. elaeagnifolium,
respectivley of C. longiareolata larvae

Bupleurum plantagineum Desf. (Apiaceae) is a plant endemic to North Africa, including Algeria. It is used as natural medicine, due to its anti-inflammatory, antioxidant and hepatoprotective properties. In this work, we studied the chemical composition of the essential oil (EO) obtained by hydro-distillation from B. plantagineum leaves collected from the national park of Gouraya (Bejaïa), Algeria. Thirty components were identified in the EO by Gas chromatography–Mass Spectrometry(GC–MS) (relative abundance: 96.7%). Monoterpene hydrocarbons (56.8%) were the predominant, followed by oxygenated monoterpenes (36.7%). The main components were cis-chrysanthenyl acetate (33.5%), α-pinene (18.4%), myrcene (16.5%) and (E)-anethole (4.9%). This volatile profile differs from those reported for other Bupleurum spp. and for B. plantagineum from Algeria, suggesting the identification of a new chemotype. Overall, our results represent a contribution to the characterisation of natural products from Algeria and the preservation of its natural biodiversity. The same results will also represent a starting point for further studies on B. plantagineum.

TOXIC EFFECT OF EUPHORBIA BUPLEUROIDES ON THE HARMFUL
INSECTS (BLATTELLA GERMANICA ; BLATTELLIDAE) AND (CULISETA LONGIAREOLATA ; CULCID)

Nouveaux taxons pour la Kabylie dans le bassin de l’oued Sahel (Djurdjura / Bibans, Algérie du nord)

Convolvulus L. and Calystegia R.Br. are two closely related genera of the Convolvulaceae family distributed in Asia, Mediterranean, Macaronesia, East Africa, and Arabia, including about 210 and 30 accepted species, respectively, of flowering plants, present as trees, shrubs, and herbs. The ethnomedical use of Convolvulus species dates to 1730s as they displayed profuse medicinal properties. In the present study, the not previously investigated chemical compositions of the essential oils from aerial parts of Convolvulus althaeoides subsp. tenuissimus (Sm.) Bat., collected in Sicily, and Calystegia sylvatica (Kit.) Griseb., collected in Algeria, were evaluated by GC-MS. The main components of the essential oil of the first one were β-caryophyllene (28.68%), γ-muurolene (23.75%), and γ-elemene (17.55%), whereas the C. silvatica essential oil was shown to be rich of valeranone (10.77%), viridiflorol (9.45%), and germacrene D (8.61%). Furthermore, a complete literature review on the ethno-pharmacological uses of Convolvulus and Calystegia species was performed.

Abstract: For a study on the vegetation of the Dreat forest, located in the chain of Western Hodna in a semi-arid zone of central Algeria. 94 stations were sampled following a subjective sampling during the 4 years 2018-2021 in order to identify the main plant species constituting the floristic procession of these ecosystems. In addition, a classification of phytosociological groups is carried out following statistical analyses (AC, AHC) to study the vegetation as well as a phytosociological typology and syntaxonomic units. This analysis showed a considerable species richness, estimated at around 471 taxa belonging to 284 genera and 66 families. Within the prospected region, the analysis of the raw phytogeographical spectrum indicates a superiority of the Mediterranean group with 259 species. The pluriregional element (or group with a wide distribution) with 114 species occupies the second chorological position followed by the northern element (Nordic group) with 60 species. Finally, the endemics with 38 species.The phytosociological study of the Dreat mountains has revealed ten plant groups belonging to six phytosociological classes : the class of Quercetea ilicis Braun-Blanquet 1947, the class of Rosmarinetea officinalis Braun-Blanquet 1947 em Rivas Martinez, Diaz, Prieto, Loidi & Penas, 1991, the class Nerio -Tamaricetea Braun-Blanquet & Bolos 1956 (1957), the class Lygeo-Stipetea Rivas Martinez 1977, em Kaabeche 1990, the class Tuberarietea guttatae Braun-Blanquet 1952 em Rivas Martinez 1977 and the class Stellarietea mediae R. TX. Lohmeyer & Preising.
Keywords : flora, phytosociology, data analysis, CA, AHC, regressive dynamics.

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Abstract
The ethno-botanical surveys carried out using the questionnaire sheets made it possible to inventory 17 medicinal species
intended for the treatment of functional intestinal disorders and to collect as much information as possible concerning local
traditional therapeutic uses in the region of Sidi Aissa. The leaves and seeds are the most used part, while the mode of
preparation differs according to the plant but the decoction and the infusion remain the most widespread mode.
These results can be considered as a source of information for scientific research in the field of phytochemistry and
pharmacology.

Phytochemistry and biological activities of an Euphorbiaceae from the Algerian Sahara.

Phytochemical and caracterization of photoprotective activity of a medicinal plant from Algeria

Bioactive compounds valorization through antibacterial investigation of medicinal plant of Algeria

L’attractivité Alimentaire de Lobopteradecipiens (Blattellidae) par Citrus sinensis (Rutaceae).

Objective: The present work aims to study the phytochemical composition and to evaluate the biological activities of R. montana L. (Rutacea) extracts.
Methods: Bioactive compounds were obtained using solid-liquid extraction using solvents of increasing polarity. The obtained extracts were qualitatively analyzed by liquid chromatography coupled with mass spectrometry (LC-MS). The pharmacological properties of R. montana were also investigated. Antioxidant activity was achieved "in vitro" using two methods: scavenging of the free radical DPPH and total antioxidant capacity. Antimicrobial activity was evaluated using disc diffusion method on 3 pathogenic bacterial strains (Escherichia Coli, Pseudomonas aeruginosa and Staphylococcus aureus). In addition, cytotoxic activity was determined by the "Brine shrimp" test.
Results: Chemical investigation allowed the identification of 14 phenolic compounds. The identified compounds were mainly phenolic acids, coumarins and flavonoids. The crude extract and the different tested fractions exhibited an interesting antioxidant activity. Chloroform extract was effective against the growth of the tested bacterial strains with zones of inhibition varying between 14 and 18 mm. In contrast, ethyl acetate and butanolic extracts were almost inactive on all of the tested bacterial strains. Furthermore, the crude extract was found to exhibit antibacterial activity with 11 to 11.66 mm of inhibitions zone. No cytotoxic effect was recorded for all the tested extracts up to a concentration of 4000 μg/mL.
Conclusion: This work highlights the potent bioactivity and acceptable drug-likeness of this plant, which supports its further uses.

Résumé
Rebbas, K., Heddad, M., Ben Si Said, Z., Haddad, S. & E. Véla (2023): Les
orchidées du Parc National de Djurdjura (Grande Kabylie, Algérie) sur
17 années - inventaire, taxonomie et biogéographie.- J. Eur. Orch. 55(1): 99-181.
L’orchidoflore de la grande Kabylie, en particulier de la réserve de biosphère du
Djurdjura et ces environs (nord-centre Algérien), demeure peu connue malgré
l’importance de ce secteur clé pour la biodiversité et fait l’objet d’un intérêt
récent des botanistes.
Cet article est un travail collaboratif initié il y a 17 ans (2006-2022) sur l’inventaire,
l’illustration, la cartographie et la taxonomie des Orchidées dans la
région du Djurdjura, nord centre de l’Algérie. Située dans le secteur de la grande
Kabylie (K1), en kabyle Tamurt Idurar, le pays des montagnes, cette région
abrite une flore diversifiée dont le recensement est encore incomplet. Avec
45 orchidées (espèces et sous espèces) et quatre hybrides sur plus de 500 stations
d’observations, cette région confirme le fort potentiel de diversité connu dans
toute la Kabylie et la Numidie.
Cette relative diversité orchidologique s’explique en grande partie par les
conditions climatiques exceptionnelles, associées à l’originalité des milieux en
altitudes de la grande Kabylie de Djurdjura, plus au moins bien conservés.
Des stations d’orchidées ont été découvertes et redécouvertes dans le Parc
National de Djurdjura. Des espèces, des sous-espèces rares et endémiques ont
été observées, identifiées et localisées, ainsi que quatre hybrides. Sept espèces
sont proposées pour être ajoutées à la protection légale au niveau national
(Cephalanthera rubra, Cypripedium calceolus, Dactylorhiza maculata subsp.
battandieri, D. markusii, Epipactis helleborine s.l., Orchis purpurea,
Platanthera bifolia subsp. kuenkelei)

Inventaire des orchidées du Parc National de Djurdjura (Grande Kabylie, Algérie).

The ornamental flora of green spaces of the University of M’sila (Pole 2) includes 32 ornamental plants (tree, shrub and herbaceous), belonging to 22 botanical families and 29 genera. The green contains 89 wild plants belonging to 29 families which find refuge at the edge and in these green spaces; of which the Asteraceae family is most abundant, in second place comes the Fabaceae and Poaceae families, then the Brassicaeae with and Euphorbiaceae on the other hand the Geraniaceae, the Plantaginaceae, the Polygonaceae are weakly represented. The chorological types of wild species show the dominance of the elements of the Mediterranean group over all other groups. The analysis of biological types of wild species reveals the dominance of therophytes over all biological types, followed by hemicryptophytes, geophytes and chamaephytes.

Diversité orchidologique du Parc National de Djurdjura (Grande Kabylie, Algérie): inventaire,
taxonomie et biogéographie

Attestation Researche gate-325 - Rebbas2023

Attestation google scholar.docx-102 - Rebbas2023

The search for new bioactive substances with anticancer activity and the understanding of their mechanisms of action are high
priorities in the research effort toward more effective cancer treatments. In this article, we analyzed, for the first time, the chemical
composition of the essential oil (EO) hydrodistilled from the aerial parts of Vicia ochroleuca Ten. (Leguminosae) by GC–MS. A total of
sixteen compounds representing 82.2% of the total composition were identified. The major compounds were phytone (20.11%),
hexadecanoic acid (10.23%), 1-octen-3-ol (9.84%), and 10-epia-cadinol (7.13%). Additionally, using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) method, the EO was tested in vitro against a panel of human cancer cells, including breast (MDA-MB 231), colon (HCT116), melanoma (A375), and
glioblastoma (T98G), with corresponding IC50 values of 23.07, 47.05, 51.64, and 64.07 lg/mL, respectively. The results demonstrate cytotoxic activity and suggest that V. ochroleuca EO could be regarded as a natural bioactive source.

Several specialized plant metabolites are reported to be enzyme inhibitors. In this investigation, the phytochemical composition and the biological activity of Rhanterium suaveolens Desf. were
studied. One new lignan (rhanteriol 1) and seven known secondary metabolites were isolated from the
aerial parts of R. suaveolens by using different chromatographic procedures. The biological properties
of the R. suaveolens extracts and the new compound were evaluated by measuring their ability to inhibit the cholinesterase and carbohydrate-hydrolyzing enzymes, using cell-free in vitro methods. The
new lignan, rhanteriol, was shown to inhibit a-amylase and a-glucosidase (IC50 = 46.42 ± 3.25 µM
and 26.76 ± 3.29 µM, respectively), as well as butyrylcholinesterase (IC50 = 10.41 ± 0.03 µM), with
an effect comparable to that of the respective standards, acarbose and galantamine. Furthermore,
docking studies were performed suggesting the interaction mode of rhanteriol with the active sites of
the investigated enzymes. The obtained data demonstrated that the aerial part of R. suaveolens could
represent a source of active molecules, such as rhanteriol, usable in the development of treatments
for preventing or treating type 2 diabetes mellitus and neurodegeneration.
Keywords: Rhanterium suaveolens Desf.; rhanteriol 1; NMR; a-amylase inhibition; a-glucosidase
inhibition; cholinesterases inhibition; molecular docking

Ethnopharmacological relevance: A rising resort to herbal therapies in Crohn’s disease (CD) alternative treatments
has been recently observed due to their remarkable natural efficiency. In this context, the weed plant Ambrosia
maritima L., traditionally known as Hachich el Aouinet in Algeria and as Damsissa in Egypt and Sudan, is widely
used in North African folk medicine to treat infections, inflammatory diseases, gastrointestinal and urinary tract
disturbances, rheumatic pain, respiratory problems, diabetes, hypertension and cancer.
Aim of the study: To assess an Ambrosia maritima L. phenolic extract for its phenolic profile composition, its
potential antioxidant activity in vitro, and its cytoprotective effect on cultured primary human endothelial cells
(ECs) stressed with H2O2 and sera from CD patients.
Materials and Methods: Phenolic compound extraction was performed with a low-temperature method. Extract
chemical profile was attained by HPLC-DAD/ESI-MS. The extract in vitro antioxidant activity was assessed using
several methods including cupric ion reducing power, DPPH radical scavenging assay, O-Phenanthroline free
radical reducing activity, ABTS cation radical decolourisation assay, Galvinoxyl free radicals scavenging assay.
Intracellular reactive oxygen species levels were evaluated in human endothelial cells by H2DCFDA, while cell
viability was assessed by MTT.
Results: The phenolic compounds extraction showed a yield of 17.66% with three di-caffeoylquinic acid isomers
detected for the first time in Ambrosia maritima L. Using different analytical methods, a significant in vitro
antioxidant activity was reported for the Ambrosia maritima L. extract, with an IC50 value of 14.33 ± 3.86 μg/mL
for the Galvinoxyl antioxidant activity method. Challenged with ECs the Ambrosia maritima L. extract showed a
biphasic dose-dependent effect on H2O2-treated cells, cytoprotective and antioxidant at low doses, and cytotoxic
and prooxidant at high doses, respectively. Viability and ROS levels data also demonstrated a prooxidant and cytotoxic effect of CD sera on cultured ECs. Interestingly, 10 μg/mL of Ambrosia maritima L. extract was able to
counteract both CD sera-induced oxidative stress and ECs death.
Conclusion: Our data indicated Ambrosia maritima L. as a source of bioactive phenolics potentially employable as a
natural alternative for CD treatment.

Observation of Helianthus annuus in the Msila region (Algeria) en 2022
La determination a été corrigée par Pr. Edward E. SCHILLING, University of Tennessee, USA " This looks like Helianthus annuus. Two traits strongly suggest this: (1) the phyllaries (involucre bracts) are ovate at the base and somewhat abruptly narrow to an attenuate apex, which distinguishes H. annuus (and H. argophyllus) from other member of the genus (in H. tuberosus and H. giganteus the phyllaries would be lanceolate, and would gradually taper from base to apex); the disk flowers have reddish lobes (these would be yellow in H. tuberosus and H. giganteus). It appears that there is some minor production of sunflower in Algeria, so this could be an escape from cultivation".
https://www.inaturalist.org/observations/147518567

the flora

La médecine traditionnelle y a sa place en Algérie, pays très riche dans sa biodiversité florale malgré l’absence de complémentarité de la phytothérapie à la médecine. Botanistes, pharmacologues, phytochimistes et médecins sont appelés à conjuguer leurs efforts et connaissances scientifiques pour que la phytothérapie soit une discipline thérapeutique officielle comme c'est le cas dans plusieurs pays (Kabouche et al, 2005). Contribution à la valorisation de certaines plantes médicinales en Algérie et dans le cadre de la recherche de nouveaux composants antioxydants et antibactériens à partir de sources naturelles, une étude photochimique sur une espèce de la flore algérienne s’est mis en place pour but d’évaluer son potentiel thérapeutique Le genre Ononis est un genre de e la famille Fabaceae considéré comme un genre appartenant à la flore algérienne et qui est représenté par plus de 75 espèces. La littérature et les travaux réalisés précédemment sur ce genre montrent sa richesse en composés bioactifs utiles en domaine thérapeutique, ce qui justifie sa grande utilisation en médecine traditionnelle

En Algérie, pays très riche dans sa biodiversité florale, la médecine traditionnelle y a sa place malgré l’absence de complémentarité de la phytothérapie à la médecine. Botanistes, phytochimistes, pharmacologues et médecins sont appelés à conjuguer leurs connaissances scientifiques pour que la phytothérapie soit une discipline thérapeutique officielle comme c'est le cas dans plusieurs pays comme la Chine, la Turquie, etc (Kabouche et al, 2005). Dans le cadre de la recherche de nouveaux composants antioxydants et antibactériens à partir de sources naturelles et de la contribution à la valorisation de certaines plantes médicinales en Algérie, nous avons concentrés notre étude phytochimiqe sur une espèce de la flore algérienne pour but d’évaluer son potentiel thérapeutique Le genre Glebionis qui relève de la famille Asteracée est un genre appartenant à la flore algérienne. La recherche bibliographique et les travaux précédents sur ce genre montrent sa richesse en composés chimiques utiles comme médicaments, ce qui justifie sa grande utilisation en médecine traditionnelle. Matériel et méthode : L’activité antiradicalaire de l’extrait hydroalcoolique de la plante du genre Glebionis a été estimée par une simple méthode colorimétrique. Ce test utilise le radical libre stable 2,2-diphényl-1-picrylhydrazyle (DPPH•) de couleur violette à l’état stable. La couleur change en jaune en présence d’antioxydant donneur d’hydrogène (Koleva et al., 2002), un volume de 1 ml de la solution de DPPH d’une absorbance de 0.750 à une longueur d’onde de 517 nm est ajouté à différentes concentrations de notre extrait. Tandis que L’activité antibactérienne de cet extrait est testée par la technique de diffusion sur disques en papier (Leulmi, 2018). Le test est réalisé vis-à-vis des différentes bactéries de Gram + et de Gram –. Résultat et discussion : Compte tenu de la complexité des processus d’oxydation et la nature diversifiée des antioxydants, avec des composants à la fois hydrophiles et hydrophobes, il n’y a pas une méthode universelle par laquelle l’activité antioxydante peut être mesurée quantitativement et d’une façon précise (Cristina et al., 2009). L’activité antioxydante ici a été évaluée par le test de DPPH, qui est l’un des tests les plus utilisés en raison de sa rapidité, sensibilité, commodité et de sa reproductibilité. L’activité antioxydante a été évaluée par spectrophotométrie à 517 nm, elle est exprimée en concentration d’inhibition IC50. Les résultats obtenus montrent qu’IC50 du témoin utilisé (trolox) est égale à 0,523 mg/ml est meilleure par rapport à notre extrait de plante Glebionis qui égale à 0,849 mg/ml. L’étude de l’activité antibactérienne de l’extraits de notre plante est testée par la méthode de diffusion des puits sur un milieu gélosé face à trois souches bactériennes à savoir Pseudomonas aeruginosa, Escherichia .coli et Staphylococcus aureus. Les résultats montrent une grande sensibilité de la douche Staphylococcus aureus face à notre extrait hydroalcoolique de la pante Glebionis avec une zone d’inhibition qui dépasse 15 mm. De même pour la souche Pseudomonas aeruginosa qui présente une forte sensibilité par une zone d’inhibition qui dépasse 16 mm face à cette plante de Glebionis. Escherichia .coli était moyennement sensible une zone d’inhibition auteur de 10 mm. La différence de la taille du halo observé entre les isolats peut refléter une production de molécules bioactives différentes ou une production des mêmes molécules avec des concentrations différentes. De ce fait, de plus amples études sont nécessaires pour confirmer l’une ou l’autre. Conclusion : Les plantes médicinales restent pour toujours une source persistante des composés bioactifs, l’exploitation de cette richesse deviens indispensable pour échapper des produits synthétisés chimiquement. L’étude faite sur cette plante du genre Glebionis montre qu’elle possède un potentiel thérapeutique qui peut être utilisée en activité antioxydante ou celle antibactérienne compte tenu des résultats de ce travail.

Mot clés : Glebionis, plante médecinale, activité antioxydante, activité antibactérienne.

The genus Physalis was first found by Linnaeusin 1753, withnin especies. However, two of the nine species are now known as Withania. Physalis species have been used in Asian and American folk medicine to treat a variety of ailments such as cancer, leukemia, dermatitis, asthma, malaria, liver disorders, and as an immunomodulatory agent. For these reasons, our work is focused on phytochemical and biological studies of the Physalis genus. Materials and methods: As a first step, our study used a face-centered central composite design (CCDC) to generate surface responses and ultrasound bath equipment to optimize three parameters (extraction time, ratio liquid/solid, and solvent concentration) to obtain the best extraction conditions, influencing total bioactive content (TPC, TFC) as well as total antioxidant activity (TAC). Results: According to our experimental design results, the highest values of responses (TPC, TFC, and TAC) were obtained under the following conditions: 70% ethanol concentration, 60 minutes of extraction time, and a 10:1 dry weight of plant ratio. Conclusion: As a result, it appears reasonable to believe that optimized ultrasonic extraction could be successfully applied to maximize the yield of antioxidant polyphenols from Physalis species.
Citation

abstract

The diversity and species richness of insects were studied at the palm grove of El Ghrous. The insects
were collected using two types of traps: Barber and Aerial. Forty-four insect species were inventoried. This
inventory shows a predominance of Order Coleoptera with 6 families, 13 species and 241 individuals, followed
by Hemiptera with 5 families, 7 species and 111 individuals, and Orthoptera with 2 families, 7 species and 54
individuals.
Key words: Insects; Oasis; Agro-ecosystem; Inventory; Biodiversity.

Cockroaches are dictyopteran insects that have existed on Earth for over 440 million years. To date, more than 4,440 species of cockroaches have been identified, many of which are considered pests and pose significant health risks to humans, such as allergies.

The fight against cockroach infestations remains a major challenge for the scientific community, particularly due to the widespread use of chemical insecticides, which contribute to insect resistance and have harmful environmental impacts. In this study, we evaluated the toxicity of aqueous extracts from three plant species: Solanum nigrum, Nicotiana glauca, and Atropa belladonna, on adult Blattella germanica. The results showed a high mortality rate, reaching up to 90% of the population after 31 days of exposure to a 1000 µg/mL dose of Solanum nigrum extract. Furthermore, the toxic effects on cockroaches were assessed based on different lethal and sublethal doses, with LD50 and TL90 values determined.

Keywords: Cockroaches, Blattella germanica, Solanum nigrum, Nicotiana glauca, Atropa belladonna, Toxic effects.

Bioctive compounds of medicinal plant from Algeria and their use as cosmetic ingredients.

Botanical surveys were carried out in spring 2022 in the wilaya of Tizi Ouzou,
allowing the rediscovery of Ophrys omegaifera subsp. hayekii and Dacylorhiza markusii.
Ecological ratings are also provided. This rediscovery will enrich the Algerian orchid flora
in general and that of the Tizi Ouzou region in particular.

Cockroaches are dictyopteran insects that appeared on earth more than 440 million years ago, with more than 4440 species of cockroaches that are known to date, many of which are considered pests and cause many problems. health issues for humans, such as allergies. The fight against cockroaches is one of the current problems that the scientific world is trying to solve, in the face of the chemicals widely used today and which cause the development of resistance in the treated insects in addition to their impact on the environment. In the present study we tested the toxicity of the aqueous extract of three plants of Solanaceae ( Solanum nigrum , Nicotiana glauca and Atropa balladona ) with regard to the adults of Blattella germanica , where we marked a high mortality rate which can reaching 90% of the population after 30 days of exposure to a dose of 1000 μg / ml of the Solanum nigrum extract , the results give a toxic effect on cockroaches determined at different lethal and sublethal times (TL50% and TL 90 %).
Keywords: Cockroaches, Blattella germanica , Solanum nigrum , Nicotiana glauca , Atropa balladona , Toxic effect.

Insect behavior is not fully defined. Biochemical stimulations of their nervous systems are caused essentially by one or many external factors whether food, sex pheromone, alarm pheromone, gregarious pheromone, and among those insects cockroaches. Many activities in the cockroach populations are based on the use of their antennae like communications and different attractiveness (food, pheromones). Our study is focused on applying a series of ethological tests in an olfactometer (“Y "tube) under red light, to test the food attractiveness of one of the forest cockroaches species belonging to the Blattellidae family, which is Loboptera decipiens (L. decipiens); Larvae, males and females. Those tests were determined under laboratory conditions by preparing hexanoic extracts using the fresh and dead leaves of Pinus halpensis (P. halpensis) in three
extraction times 15min, 30min, and 60min. From the obtained results, we were able to indicate that most individuals of L. decipiens have detected the smell of food source, after a detection time that lasts and differs from one stage to another and from one dose of odor to another. These results show that the larval or adult individuals (males and females) are attracted to the hexanoic extracts of fresh leaves of P. halpensis with a percentage ranging from 80% to 100% for females, 73.33% to 86.67% for males and 73.33% to 93.33% for thve larvae. And the dead leaves attract the females from 66.67% to 73.33% and 66.67% of males and 73.33% to 80% for larvae.
Keywords: Food attractiveness, Forest cockroach, Loboptera decipiens, Pinus halpensis.

Résumé :
Introduction :
En Algérie, pays très riche dans sa biodiversité florale, la médecine traditionnelle y a sa place malgré l’absence de complémentarité de la phytothérapie à la médecine. Botanistes, phytochimistes, pharmacologues et médecins sont appelés à conjuguer leurs connaissances scientifiques pour que la phytothérapie soit une discipline thérapeutique officielle comme c'est le cas dans plusieurs pays comme la Chine, la Turquie, etc (Kabouche et al, 2005). Dans le cadre de la recherche de nouveaux composants antioxydants et antibactériens à partir de sources naturelles et de la contribution à la valorisation de certaines plantes médicinales en Algérie, nous avons concentrés notre étude phytochimiqe sur une espèce de la flore algérienne pour but d’évaluer son potentiel thérapeutique Le genre Glebionis qui relève de la famille Asteracée est un genre appartenant à la flore algérienne. La recherche bibliographique et les travaux précédents sur ce genre montrent sa richesse en composés chimiques utiles comme médicaments, ce qui justifie sa grande utilisation en médecine traditionnelle.
Matériel et méthode :
L’activité antiradicalaire de l’extrait hydroalcoolique de la plante du genre Glebionis a été estimée par une simple méthode colorimétrique. Ce test utilise le radical libre stable 2,2-diphényl-1-picrylhydrazyle (DPPH•) de couleur violette à l’état stable. La couleur change en jaune en présence d’antioxydant donneur d’hydrogène (Koleva et al., 2002), un volume de 1 ml de la solution de DPPH d’une absorbance de 0.750 à une longueur d’onde de 517 nm est ajouté à différentes concentrations de notre extrait. Tandis que L’activité antibactérienne de cet extrait est testée par la technique de diffusion sur disques en papier (Leulmi, 2018). Le test est réalisé vis-à-vis des différentes bactéries de Gram + et de Gram –.
Résultat et discussion :
Compte tenu de la complexité des processus d’oxydation et la nature diversifiée des antioxydants, avec des composants à la fois hydrophiles et hydrophobes, il n’y a pas une méthode universelle par laquelle l’activité antioxydante peut être mesurée quantitativement et d’une façon précise (Cristina et al., 2009). L’activité antioxydante ici a été évaluée par le test de DPPH, qui est l’un des tests les plus utilisés en raison de sa rapidité, sensibilité, commodité et de sa reproductibilité. L’activité antioxydante a été évaluée par spectrophotométrie à 517 nm, elle est exprimée en concentration d’inhibition IC50. Les résultats obtenus montrent qu’IC50 du témoin utilisé (trolox) est égale à 0,523 mg/ml est meilleure par rapport à notre extrait de plante Glebionis qui égale à 0,849 mg/ml. L’étude de l’activité antibactérienne de l’extraits de notre plante est testée par la méthode de diffusion des puits sur un milieu gélosé face à trois souches bactériennes à savoir Pseudomonas aeruginosa, Escherichia .coli et Staphylococcus aureus. Les résultats montrent une grande sensibilité de la douche Staphylococcus aureus face à notre extrait hydroalcoolique de la pante Glebionis avec une zone d’inhibition qui dépasse 15 mm. De même pour la souche Pseudomonas aeruginosa qui présente une forte sensibilité par une zone d’inhibition qui dépasse 16 mm face à cette plante de Glebionis. Escherichia .coli était moyennement sensible une zone d’inhibition auteur de 10 mm. La différence de la taille du halo observé entre les isolats peut refléter une production de molécules bioactives différentes ou une production des mêmes molécules avec des concentrations différentes. De ce fait, de plus amples études sont nécessaires pour confirmer l’une ou l’autre.
Conclusion :
Les plantes médicinales restent pour toujours une source persistante des composés bioactifs, l’exploitation de cette richesse deviens indispensable pour échapper des produits synthétisés chimiquement. L’étude faite sur cette plante du genre Glebionis montre qu’elle possède un potentiel thérapeutique qui peut être utilisée en activité antioxydante ou celle antibactérienne compte tenu des résultats de ce travail.
Mot clés : Glebionis, plante médecinale, activité antioxydante, activité antibactérienne.

The study aimed at identifying the plants of Bousaada district of M'sila province, South East Algeria, used by the local people as traditional medicines and analyzing their use value. The ethnomedicinal use information was collected by interviewing local informants using structured questionnaires through regular field surveys. A total of 193 species belonging to 69 families was identified. Lamiaceae and Asteraceae were the most commonly reported medicinal plants with 85 and 71 species, respectively. Artemisia herba-alba and Juniperus oxycedrus were the most widely used plants as the traditional medicine by the local population. The highest use value (UV) was observed for Citrus lemon (L.) Burm., Ficus carica L., Moringa oleifera Lam. and Olea europaea L. (UV=5). The highest fidelity level (FL) value was for 73 species. The calculated informant consensus factor (ICF) showed that diseases related to gastrointestinal disorders and diseases of the glands attached to the digestive system diseases present the highest values.

Inventaire et cartographie des Orchidées de la
région de M’Sila (Algérie)

DIVERSITY AND TRADITIONAL USE VALUE OF MEDICINAL PLANTS IN BOU
SAADA DISTRICT OF M'SILA PROVINCE, SOUTH EAST ALGERIA

Etude du pouvoir antioxydant et effet antibactérien d'une plante appartenant à la flore algerienne

Etude ethnobotanique des plantes médicinales d'Ouanougha , M'sila

Contribution à l'inventaire des orchidées dans la region de Tizi Ouzou

Conférence sur : la botanique au service de la science

EVALUATION OF THE TOXICITY OF THE AQUEOUS EXTRACT OF THE LEAVES OF A PLANT
(ASTERACEAE) ON THE LARVAE OF Culiseta longiareolata (DIPTERA, CULICIDAE)

THE INFLUENCE OF THE CLEOME ARABICA PLANT (CAPPARIDACEAE) ON THE FOOD
ATTRACTIVENESS OF BLLATTELLA GERMANICA AND BLLATTA LATERALIS (BLATTELLIDAE)

INVENTAIRE ET CARTOGRAPHIE DES ORCHIDÉES DE LA RÉGION
DE M’SILA (ALGÉRIE)

IMPACT OF THE PLANT CLEOME ARABICA (CAPPARIDACEAE) ON THE MORTALITY
OF TWO URBAN COCKROACHES BLATTELLA GERMANICA AND BLATTA LATERALIS

STUDY OF THE TOXIC EFFECT OF DIFFERENT CONCENTRATIONS OF
THE PLANT NICOTIANA GLAUCA (SOLANACEAE) ON BLATTELLA
GERMANICA (BLATTELLIDAE)

Inventaire préliminaire , écologie et enjeux de conservation des orchidées de la wilaya de Tizi ouzou (
Algérie )

IMPACT OF THE PLANT SOLANUM NIGRUM L (SOLANACAE) ON
BLATTELLA GERMANICA (BLATTELIDAE)

Les Orchidées de la Wilaya de M’Sila (Hodna, Algérie) : inventaire, écologie et biogéographie

Biodivérsité orchidologique de la Grande Kabylie ( Tizi ouzou )

In-vitro antibacterial and antifungal effects of Physalis acutifolia L. recently
discovered in Algeria

The anti-diarrheal medicinal flora of the Ouanougha region includes 41
species belonging to 20 botanical families and 35 genera. These families are mainly
dominated by Lamiaceae (8 species), Anarcadiaceae, Fabaceae, Myrtaceae,
Plantaginaceae and Rosaceae (3 species) and eight families containing two species and the
other families are represented by one species. The leaves are the most used organ.
The methods of preparation most frequently employed for the majority of remedies are
decoction and infusion.
Keywords: Anti-diarrheal plant, inventory, ethnobotany, chorology, M'sila, Algeria.

The flora of the cereal fields and of the olive groves of Hammam Dalaa has
116 species belonging to 27 botanical families and 94 genera. Note the presence of native
flora which finds refuge at the edge of the fields. Three families, the Asteraceae, the
Poaceae and the Brassicaceae respectively dominate. Mediterranean floristics predominate
and characterize the wild flora of the fields. Species biological types reveal the dominance
of therophytes over all biological types, followed by hemicryptophytes and geophytes.
Keywords: weed flora, plants of economic interest, inventory, chorology, M'sila, Algeria

The authors present the unpublished inventory of epiphytic lichens in the cedar
forests of the Hodna region. This inventory constitutes a database of the lichens of the
Hodna mountains in particular and an enrichment of the list of Algerian lichenoflora in
general. Thirty stations spread over three study areas were studied and characterized by
the calculation of various parameters. After the collection and identification of the lichen
samples, the study area is home to 51 lichen taxa spread over 14 families, the most
represented of which are the Physciaseae (16 species), Parmeliaceae (07 species),
Lecanoraceae (06 species), Ramalinaceae (06 species).
From a physiognomic point of view, foliose lichens are the most dominant with 44.31%
followed by crustaceans 34.09 %, fruticose 15.36 % and finally scaly and gelatinous and
leprous with respectively 02.27 %, 02.27% and 01.13 %. Specifically, the 31 lichen species
are present on cedar, with a clear dominance of the genus Physcia and Parmelia followed
by the genus Lecanora then Ramalina. From the lichen floristic richness point of view, the
cedar forest represents a ratio of 31/51 species (60.78 %) of the local lichen diversity.
The Ouanougha cedar forest is richer than the Maadid cedar forest, this can be partly
explained by the fact that the latter is much more degraded (in advanced decline) than the
first which benefits from the humid influences of Djurdjura.
Keywords:Inventory, Lichens, Cedars, Hodna Mountains, Algeria

Our study focuses on the valuation of Spirulina, which can contain up to 70%
of protein, in the manufacture of a dietary product. It aims to elaborate a hyper-protein
energy drink of grape juice having a significant nutritional intake .Our study aims to show
the effect of the incorporation of spirulina on the physicochemical parameters of a grape
juice at different doses (0.1g/l - 0.3g/l - 0.5g/l - 0.7g/l) compared to a control drink and
evaluation of its nutritional value. Several analyzes were carried out The Physicochemical analyzes - acidity, Brix, pH, and density with reference to Algerian standards, as
well as sensory analyzes ;stability testing .The statistical analysis ( Newman-keuls test)
showed that they are not significantly different between all parameters at the probability
0.05 probability level. The physico-chemical analyses obtained from different products are
in agreement with Algerian standards.Indeed, the juice has a certain richness in proteins
(31%); it is therefore considered a high protein product. A good microbiological quality of
the juice was revealed. The F3 formulation (0.5g) is judged to be good, and well
appreciated by the tasting panel. Its stability test for 4 weeks at 22°C showed that
formulation F3 (0.5g) is a stable juice. There is a significant effect ( p ˂ 100) of the dose of
spirulina incorporated on the parameters studied ( Acidity, pH, Density, refractive index ).
The enrichment of grape juice by spirulina allowed us to obtain a dietetic grape juice, a
stable and hyper protein with a protein level of 31%.
Keywords: Enrichment, Grape juice, Spirulina, Stability test, Nutritional value.

The plant Thapsia garganica belongs to the family Apiaceae and is widespread
in the Mediterranean basin. It is considered one of the most famous medicinal plants since
ancient times. We started this work with the methanolic extraction of all the components
contained in the plant in order to obtain a crude extract, which we separated with different
polar solvents “petroleum ether, chloroform, and ethyl acetate and n-butanol”
The flavonoids were evaluated by the method of aluminum chlorides AlCl3, the content is
estimated at 5.6009 mg EQ / g dry matter in the crude extract.
We extracted the essential oils using a Clevenger device, and then we studied the
antimicrobial and antioxidant activity of all extracts and compared them among
themselves.
Key words: medicinal plants, antioxidant activity, antibacterial activity

The ethno-botanical surveys carried out using questionnaires in the region of
Hammam Dalaa made it possible to inventory 68 medicinal species belonging to 35 botanical
families and to collect as much information as possible concerning local traditional therapeutic
uses. This study also showed that the leaves are the most used part. Recipes are basically prepared
by decoction or powder. Of all the diseases treated, digestive disorders are the most frequent.
These results are considered as a source of information for scientific research in the field of
phytochemistry and pharmacology.
Keywords: M'Sila, Hammam Dalaa, medicinal plants, ethnobotany.

Volutaria lippii (L.) Cass. ex Maire, syn. Centaurea lippii (L.),
(Asteraceae) is a plant from the central region of Algeria, considerably distributed in all Mediterranean areas. Herein, the antioxidant activity of the three derived fractions [chloroform (CHCl3),
ethyl acetate (EtOAc) and n-butanol (n-BuOH)] of the 70% methanol extract of the aerial parts (leaves and flowers), was assessed
by using CUPRAC, ABTS, DPPH free radical scavenging, and
b-carotene bleaching methods. The results obtained allowed to
guide the fractionation of EtOAc and n-BuOH fractions by CC followed by purification by TLC and reverse phase HPLC. A guaianolide glucoside, 3b-hydroxy-11b,13-dihydrodehydrocostuslactone
8a-O-(6’-acetyl-b-glucopyranoside) (1), never reported in the literature, was isolated together with other known compounds (2–14).
Their structures were elucidated by the extensive use of 1 D- and
2 D-NMR experiments along with ESI-MS analyses and with comparison with literature data.

Guizotia abyssinica (L. f) Cass. (Asteraceae) est originaire d’Ethiopie. Cette astéracée a été
découverte dans la région de Sétif et de M’Sila, dans le nord-est de l’Algérie. Des notes
écologiques et d’utilisations alimentaires sont également fournies.

The present research aimed to carry out the phytochemical analysis, in vitro
antioxidant activity, enzymes inhibition and anti-inflammatory effect of the
selective extracts of the Algerian L. multifida from two regions; Msila region
and Constantine region. The total phenolic and flavonoids contents of crude
extract (CE) and its solvent partition fractions: dichloromethane (DME), ethyl
acetate (EAE) and n-butanol (BUE) were determined spectrophotometriclly. The
antioxidant activity of extracts was achieved by the use of seven methods and
the enzyme inhibitory activity of extracts was evaluated against α-amylase and butyrylcholinesterase. Moreover, the in vivo anti-inflammatory activity of the
aqueous extract of L. multifida from Constantine region was evaluated using paw
edema model. The M.EAE present the highest TPC and TFC, which were about
462.23±11.74μg GAE/mg extract and 125.90±0.16μg QE/mg extract, respectively.
In addition, the M.EAE exhibited an excellent antioxidant activity, as it has a
great ability to scavenging the DPPH, ABTS and galvinoxyl free radicals, as well as
reducing power and metal chelating. However, the M.CE and C.CE showed the best
inhibitory activity of the α-amylase butyrylcholinesterase (IC50=64.17±1.81μg/
ml and 83.55±1.97μg/ml, respectively. The preliminary investigation reveals that
the EAE has a good bio-pharmacological activity, which it possesses an interesting
potential for pharmaceutical/nutraceutical applications.
Key words: Antioxidant activity, anti-inflammatory effect, enzymes inhibition,
Lavandula.

La présente étude est pour but de valoriser les bio déchets du restaurant universitaire Hasnaoua II de
l'université Mouloud Mammeri de Tizi-Ouzou avec les déchets de palmier prévenant du jardin d’essai ElHamma (Alger), ainsi que le marc de café obtenue auprès des cafétérias à proximité de l'université et cela par
la technique de compostage.
Deux composts ont été réalisés, un à l’air libre et aux températures ambiantes, l’autre au laboratoire afin de
contrôler les paramètres de compostage.
Le pH, la teneur en matière organique, la teneur en carbone organique et le test de germination ont été
mesuré pour les deux compostes. Les résultats de nos analyses montrent que le pH des deux compostes sont
alcalin.
Le composte réalisé à l’air libre à une teneur élevée en matière organique tandis que celui réalisé au
laboratoire est pauvre en matière organique. Les deux échantillons de composte présentent des taux de
germination élevés.
MOTS-CLE : Déchets, Compostage, Analyse biologique, Environnement, Tizi Ouzou .

ABSTRACT. For the first time, this work is devoted to the phytochemical and biological study of a
medicinal plant belonging to the Algerian flora Lathyrus latifolius. This research was conducted to
assess the phytochemical composition of ethyle acetate ,butanolic and chloroform extracts using
LCMS/MS, following by testing in vitro antioxidant ability using DPPH, ABTS·+, O2 − DMSO
alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. The liquid chromatography
results showed that ethyle acetate extract have a high amount of Hesperidin (583.31 g/ml) and
Quercetin-3-D-xyloside(27.467 g/ml), while the amounts present in the butanolic extract are
respectively (3.360 g/ml) and (1.812g/ml). Furthermore, butanol and ethyle acetate extracts had
good antioxidant activity in all tests used. Indeed, the presence of phenolic compounds may contribute
to their antioxidant activity.

The synthesis of pesticides has advanced significantly over the past century. Synthetic
pesticides, on the other hand, have swiftly proven to be aggressive toward non-target creatures, such as
helpful insects, animals, and people, in addition to their persistence in nature, which upsets ecological
balances. This paper describes the toxic effects of Ruta chalepensis (Rutaceae) ethanolic extract on
Drosophila melanogaster courtship and course of sexual behavior, mortality of Drosophila melanogaster
larvae (determination of lethal concentrations and lethal times), and choice of oviposition environment and
reproduction in this fly's adults. The plant's aqueous extract inhibits both mating and reproduction.
KEYWORDS: Drosophila melanogaster, Ruta chalepensis, ethanolic extract, mortality, sexual behavior,
oviposition.

Résumé
L’objectif de notre travail était de valoriser les bio-déchets du restaurant Hasnaoua II avec les déchets de palmiers des jardins d’essai El Hamma par la technique de compostage, la mise en place des déchets du restaurant a été effectuée juste avant le confinement puis laissés se composter tout seul sans suivi ; en revanche nous avons comparé les paramètres physico-chimiques entre notre échantillon et l’autre réalisé par notre promoteur le professeur Derridj Arezki, par la suite nous avons effectué une analyse comparative en conclusion.
A partir de ce travail, nous avons effectué des analyses de paramètres suivants : le pH, la teneur en matière organique, la teneur en carbone organique, et le test de germination.
Ainsi, on a effectué des analyses sur 02 échantillons :
1. Echantillon 01 : compost non suivi, Le pH a atteint 7,57. Il contient des teneurs de carbone 27,58 %, de matière organique 47,44 %, et 74 % comme taux de germination.
2. Echantillon 02 : compost suivi et retourné, Le pH a atteint 8.07. Il contient des teneurs de carbone 8.23 %, de matière organique 14.6 %, et 64 % comme taux de germination.
Les mots clés : compost, restaurants universitaires, d’entreprise, débits feuilles de palmiers, Algérie.

This work presents a preliminary synthesis of unpublished observations of orchids carried out between June 2021 and April 2022 in the region of Tizi Ouzou and those of second author (RK) carried out between 2010 - 2019 in the Djurdjura National Park.
The lack of information on the distribution areas of orchids in the wilaya of Tizi Ouzou, let us opt for a method of subjective sampling that directly targets the place of development of orchids as had already done De Belair et al. (2005) for the whole Numidia, Rebbas & Véla (2021) on a transect Kabylie - Monts de Hodna and Rebbas et al. (2021) in the wilaya of Béjaia.
On the stations (Ait Zikki, Azrou n'thor, Tirourda, Tikjda, Djurdjura National Park, Ouacif, Chellata, Akfadou) that were prospected, we inventoried the following orchids: Anacamptis coriophora (L.) R.M.Bateman, Pridgeon & M.W.Chase subsp. fragrans (Pollini) Bateman, Pridgeon & Chase, Anacamptis papilionacea subsp. expansa (Ten.) Amard. & Dusak, Anacamptis pyramidalis (L.) Rich. subsp. pyramidalis, Androrchis mascula (L.) D.Tyteca & E.Klein subsp. maghrebiana (B.Baumann & H.Baumann) W.Foelsche & Jakely, Androrchis olbiensis (Reut. ex Gren.) D.Tyteca & E.Klein, Androrchis pauciflora (Ten.) D.Tyteca & E.Klein subsp. laeta (Steinh.) Véla, Rebbas & R.Martin, Cephalanthera longifolia (L.) Fritsch, Cypripedium calceolus L., Dactylorhiza elata (s.l.), Dactylorhiza maculata subsp. battandieri (Raynaud) H.Baumann & Künkele, Epipactis helleborine (L.) Crantz, Himantoglossum hircinum (L.) Spreng. subsp. hircinum, Ophrys apifera Huds., Ophrys atlantica Munby subsp. atlantica, Ophrys battandieri E.G.Camus, P.Bergon & A.Camus, Ophrys fusca s.l., Ophrys lutea Cavanilles subsp. lutea, Ophrys numida Devillers-Tersch. & Devillers, Ophrys omegaifera subsp. hayekii (H. Fleischm. & Soo) Kreutz, Ophrys speculum Link subsp. speculum, Ophrys tenthredinifera subsp. ficalhoana (J.A.Guim.) M.R.Lowe & D.Tyteca, Orchis anthropophora (L.) All., Orchis italica Poir, Spiranthes spiralis (L.) Chevall.
This contribution to this inventory does not stop here, it will be necessary to extend our orchidological surveys on other stations (communes) of the wilaya of Tizi Ouzou during the year 2022 to evaluate their state of conservation.

Key words: Orchids, inventory, ecology, endemism, conservation, Tizi Ouzou.


Translated with www.DeepL.com/Translator (free version)

Notre travail présente une synthèse préliminaire des observations inédites des orchidées effectuées entre juin 2021 et avril 2022 au niveau de la Wilaya de Tizi Ouzou et celles des auteurs (RK, VE) effectuées entre 2010 - 2020 au parc national de Djurdjura.
L'insuffisance d'informations sur les aires de répartition des orchidées de la wilaya de Tizi Ouzou, nous laisse opter pour une méthode d'échantillonnage aléatoire qui cible directement le lieu de développement des orchidées comme l'avaient déjà effectué De Belair et al. (2005) pour l'ensemble de la Numidie, Martin et al. (2020) pour la Kabylie, Numidie et Aurès, Rebbas & Véla (2021) sur un transect Kabylie – Monts de Hodna et Rebbas et al. (2021) dans la wilaya de Béjaia.
Sur les stations (Ait Yahia, Ait Toudert, Ait Zikki, Makouda, Tirourda, Akfadou, parc national de Djurdjura, Ouacif…) qui ont été prospecté, nous avons inventorié les Orchidées suivantes : Anacamptis coriophora (L.) R.M.Bateman, Pridgeon & M.W.Chase subsp. fragrans (Pollini) Bateman, Pridgeon & Chase, Anacamptis papilionacea subsp. expansa (Ten.) Amard. & Dusak, Anacamptis pyramidalis (L.) Rich. subsp. pyramidalis, Androrchis mascula (L.) D.Tyteca & E.Klein subsp. maghrebiana (B.Baumann & H.Baumann) W.Foelsche & Jakely, Androrchis olbiensis (Reut. ex Gren.) D.Tyteca & E.Klein, Androrchis pauciflora (Ten.) D.Tyteca & E.Klein subsp. laeta (Steinh.) Véla, Rebbas & R.Martin, Cephalanthera longifolia (L.) Fritsch, Dactylorhiza elata (s.l.), Dactylorhiza maculata subsp. battandieri (Raynaud) H. Baumann & Künkele, Epipactis helleborine (L.) Crantz, Himantoglossum hircinum (L.) Spreng. subsp. hircinum, Ophrys apifera Huds., Ophrys atlantica Munby subsp. atlantica, Ophrys battandieri E.G.Camus, P.Bergon & A.Camus, Ophrys fusca s.l., Ophrys lutea Cavanilles subsp. lutea, Ophrys numida Devillers-Tersch. & Devillers, Ophrys omegaifera subsp. hayekii (H. Fleischm. & Soo) Kreutz, Ophrys scolopax subsp. apiformis (Desf.) Maire & Weiller, Ophrys scolopax Cav. subsp. scolopax, Ophrys speculum Link subsp. speculum, Ophrys tenthredinifera subsp. ficalhoana (J.A.Guim.) M.R.Lowe & D.Tyteca, Ophrys tenthredinifera subsp. tenthredinifera, Orchis anthropophora (L.) All., Orchis italica Poir., Serapias lingua L. subsp. lingua, Serapias parviflora Parl., Serapias strictiflora Welw. ex Veiga., Spiranthes spiralis (L.) Chevall.f

Mots clés : Orchidaceae, inventaire, endémisme, conservation, Tizi Ouzou, Afrique du Nord.

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Mosquitoes are the most terrifying both for their abundance and for the diseases they transmit. They are vectors of malaria, lymphatic filariasis and arbovirus such as yellow fever, dengue, viral encephalitis and African horse sickness. These characteristics give this fauna prominent importance and health interest. To identify the most widespread mosquito species, we conducted for the first time an inventory in the Aures region more particularly the wilaya of Batna (East of Algeria) from May 2017 to January 2020, we studied their diversity, abundance and distribution. The sampling is done by monthly surveys of the various localities chosen, using the dipping method to collect the larvae. In the various sites prospected, we were able to collect a total of 8275 mosquito individuals presented by nine species belonging to four different genera: Culiseta, Culex, Aedes and Anopheles of which the genus Culiseta is the best represented, particularly with the species Culiseta longiareolata (52.07%). It appears from what we have obtained that the Batna region reveals a significant diversity of mosquitoes. This study aimed to inventory the Culicidian species more broadly by surveys in several localities of Batna region at the level of different environments. This will allow us to know the faunistic composition of the region, to collect and record information on the vectors, their habitats and the conditions that favors their multiplication within a given area

Les macro-lichens de la forêt d’Errich (Bouira, Algérie)

Inventaire des lichens des cédraies de la région de Hodna

Orchidées d’Algérie : inventaire et cartographie sur
un transect Kabylie – Monts de Hodna

Le genre Allium en Algérie : inventaire
et illustration d’un défi taxonomique

Utilisation d'une plante sahaerienne Cleome arabica chez le Rat Wistar

Impactes des odeurs alimentaires de Cleome arabica sur l'attractivité de Drosophila

Ethnobotanique et phytomedicine des plantes médicinales de la région de Dreat, M'sila

Valorisation des extraits éthanolique de une plante saharienne Cleome arabica (Capparidaceae) sur le
comportement alimentaire des mouches de vinaigre Drosophila melanogster (Diptera ; Drosophilidae).

Effets biotoxiques des extraits de Cleome arabica sur le rat wistar

L’influence d’une plante médicinale Ruta chalepensis contre une espèce nuisible Blattela
germanica (Blattellidae)

Rebbas K. 2021 Contribution par des photos des orchidées d'Algérie -Beiträge zur Gattung Ophrys

Valorisation de l’effet toxique des différentes doses de la plante Ruta chalpensis sur les femelles de Blattella
germanica ; (Blattellidae

Evaluation des paramètres biochimiques seriques chez rat wistar traités par des extraits éthanoliques des feuilles de Cleome arabica

L’impact des molécules bioactives des extraits aqueux de Ruta chalepensis (Rutaceae) sur Blattella germanica

Effets toxicologiques des grains de Solanum sodomaeum (Solanaceae) sur les mâles de Blattella germanica
(Dictyoptera ; Blattellidae)

Evaluation des molécules bioactives issues de l’extrait éthanolique de Cleome arabica (Capparidaceae): Effets
sur la mortalité et le développement de Drosophila melanogaster (Diptera ; Drosophilidae)

Etude ethnobotanique de quelques plantes médicinales de la région de Hammam Dhalaa
(M’sila, Algérie)

LA MISE EN VALEURE DE L'EXTRAIT AQUEUX DE PHYSALIS ACUTIFOLIA
(MIRES) COMME UN BIOINSECTICIDE CONTRE CULISETA
LONGIAREOLATA(CULICIDAE)

Contribution à l’inventaire préliminaire des Orchidées dans la wilaya de Tizi Ouzou.

Diversité orchidologique de la région de Tizi Ouzou : inventaire et écologie.

Effets toxiques et immunologiques de l’extrait aqueux de la plante saharienne Cleome arabica (Capparidaceae) sur le rat wistar.

Allelopathic potentiality of Pituranthos scoparius essential oil on germination of some crop plants.

Chemical composition, antioxidant and phytotoxic activity of Elaeoselinum fontanesii essential oils.

La loi algérienne et les organismes génétiquement modifiés (OGM).

Phenolic Profile and biological Activity of a plant from M’sila.

Phytochemical investigation of Phlomis herba venti L.

Découverte de nouvelles stations de Centaurea hyalolepis Boiss. en Algérie.

Inventaire des plantes anti-diarrhéiques de la région de Hammam Dalaa (M’sila, Algérie).

Evaluation des extraits aqueux et éthanolique de Cleome arabica (Capparidaceae) sur le comportement d’oviposition de Drosophila melanogaster (Diptera ; Drosophilidae).

Inventaire de la faune culicidienne dans la région d’El Hamel (M’sila, Algérie).

Découverte des nouvelles stations d’Orchidées dans la région d’Ouanougha (M’sila, Algérie).

Contribution à l’étude ethnobotanique des plantes médicinales sur un transect M’sila-Djelfa

Inventaire des plantes à pollen allergisant dans la région de Maadid (M’sila, Algérie).

Flore et plantes médicinales de la région de Dréat (M’sila, Algérie).

Flore adventice des cultures de la région d’Ouanougha (M’sila, Algérie): inventaire, chorologie et systématique.

Inventaire des plantes messicoles des champs de culture de la région de Hammam Dalaa (M'sila, Algérie).

Inventaire floristique du massif montagneux de Maadid (M’Sila, Algérie).

Bioénergie à base de déchets de phyto-entretiens des milieux urbains et périurbains des Hautes Plaines Sétifiennes.

Lichenoflore du massif de Guezoul (Tiaret, Algérie) : inventaire, chorologie et systématique.

Orchidées d’Algérie : inventaire et cartographie sur un transect Kabylie – Monts de Hodna. Conference.

Le genre Allium en Algérie : inventaire et illustration d’un défi taxonomique.

Medicinal plants are used all over the world and have rapidly gained economic importance. The
study was conducted to investigate the effects of the aqueous extract of C. arabica leaves on the sexual
behavior of Wistar rats. C. arabica is a medicinal plant with a foul odor, toxic, and has hallucinogenic effects.
The experimental study was carried out on white rats (male and female) of the Wistar strain from the Pasteur
Institute of Algiers (Kouba, Algeria) weighing between 150 and 200g sexually naive. The animals are raised
in polyethylene cages, the rats were divided into 4 groups (n=10 rats/group) which received a saline solution
(male and female control group), 0.17 µg/ml of the aqueous extract of C. arabica leaves for 7 days orally
(male and female treated group). The sexual behavior test was carried out according to four crosses types.
The results of the treated groups showed a significant decrease in the mating frequency compared to the
control group. Overall, the results showed that C. arabica significantly altered the sexual behavioral
parameters (mounts frequency, mating time, licking time, first contact time, contacts number, intromission,
latency time, and frequency). The aqueous extract of the plant caused an inconsistent decrease in the
parameters of sexual behavior and orientation activity that were recorded in the treated animals. The C.
arabica leaves aqueous extract caused an inconsistent decrease in the sexual behavioral parameters and
orientation activity that were recorded in the treated animals.
Keywords: Rattus rattus, sedative effect, sexual behavioral parameters, C.arabica.

The use of pesticides is becoming an indispensable technique in most agricultural practices,
regardless of the level of development of the country. Currently, the pesticides used are often
less toxic and more specific and are based on insect physiology. Recent studies indicate that
pesticide intoxication induces oxidative stress. This work aims to study the neurobehavioral
consequences of the administration of two insecticides currently widely used in agriculture:
spirotetramat (an inhibitor of lipid synthesis in insects) and the ethanolic extract of Citrullus
colocynthis (a plant endemic to the Algerian Sahara) and the ethanolic extract of Cleome
arabica (Capparidaceae) The different behavioral tests (elevated cross maze, open fields and
forced swimming) show that the have a significant impact on the degree of anxiety of
rodents.These products significantly influence the biochemical parameters (blood sugar,
cholesterol, triglycerides, urea, creatinine), the hormone Adreno CorticoTropic Hormone,
acetylcholine esterase, immune parameters and organ weight.

The Dréat area has an exceptional phytodiversity. The preservation, conservation and enhancement of this natural
wealth require an inventory of existing plants and ethnobotanical surveys. This floristic and ethnobotanical study was carried
out with the aim of carrying out the most complete inventory possible of the medicinal plants of Dréat and of gathering
information concerning the therapeutic uses practiced in the region. Using a questionnaire, the series of ethnobotanical
surveys carried out in the region enabled us to inventory 81 species belonging to 35 families and 73 genera.
The foliage and the seed are the two most used parts. The majority of therapeutic remedies are prepared in the form of a
decoction. In terms of diseases treated, digestive disorders occupy the first place, followed by urogenital conditions,
respiratory disorders, nervous system disorders, skin conditions and circulatory disorders having the same percentage, hair
care, the rest includes other diseases with a different rate.
Keywords: Medicinal plants, Ethnobotanical investigation, Preservation, Valorisation, Phytotherapy.

Les auteurs signalent la découverte de nouvelles stations de Centaurea hyalolepis Boiss. dans plusieurs régions
d’Algérie. Les notes écologiques sont également fournies.
Mots clés: Centaurea hyalolepis, découverte, nouvelles stations, Afrique du Nord.

Because of the environmental problems and dangers to human health caused by chemical
insecticides, the use of natural biocides seems to be imperative. In this context toxicity tests were
carried out according to the protocol of the World Health Organization (WHO) for six aqueous
extracts of leaves of: Ambrosia Maritima, Hertia centifolia, Xanthium strumarium, Datura
stramonium, Solanum elaeagnifolium, and Salvea verbena, with a series of three doses for each
extract. The evaluated extracts showed good larvicidal activity against the fourth instar larvae of
Culiseta longiareolata mosquito, the decoction method is used for the preparation of extracts. The
mortality rate increases depending on the concentration of the used extract and the exposure time,
with interesting lethal concentrations LC50% and LC90%, A. Maritima (11.65 μg / ml and 52.40 μg
/ ml) after 120 hours and D. stramonium (16.94 μg / ml and 28.36 μg / ml) after 96 hours. While the
lethal times LT50% and LT90% do not exceed (0,01 day to 2,14 day) at a dose of 219.9 μg / ml of S.
elaeagnifolium (0,95 day to 1,34 day) with a dose of 160 μg / ml of H. centifolia.

Ce travail a pour objectif de déterminer les usages traditionnels des plantes
médicinales dans la zone de Djebel Djedoug située au nord de la région de M’sila
(Algérie). Cette zone renferme une flore médicinale riche et diversifiée. Les
enquêtes ethnobotaniques réalisées à l'aide des fiches questionnaires ont
permis d'inventorier 64 espèces médicinales appartenant à 35 familles
botaniques, et de collecter le maximum d'informations concernant les usages
thérapeutiques traditionnels de la population locale. Par ailleurs, les résultats
peuvent être considérés comme une source d'information pour les recherches
scientifiques dans le domaine de la phytochimie et la pharmacologie

La section Melanocrommyum Webb & Berthel. du genre Allium L., de barycentre irano-anatolien, a longtemps été considérée sous une seule espèce en Europe de l’ouest et au Maghreb, à savoir la messicole archéophyte Allium nigrum L.. La découverte d’Allium cyrilli Ten. en Algérie vient confirmer la coexistence des deux espèces au Maghreb, comme cela a été récemment mis en évidence en France et en Espagne. Nous donnons une comparaison détaillée et illustrée des deux espèces à partir de récoltes
et de photos effectuées in situ en Algérie. A travers une révision des parts historiques des herbiers P et MPU, nous avons pu écarter l’hypothèse d’une éventuelle présence ancienne d’A. cyrilli sous une appellation erronée. L’espèce semble donc d’apparition relativement récente dans les cultures d’altitude en Algérie. Par ailleurs une régression d’A. nigrum dans les cultures de plaine paraît probable. Nous proposons enfin un complément de clé d’identification basé sur le modèle de la flore d’Algérie
de Quézel & Santa.

Characterizing genetic diversity and structure of populations is essential for the effective
conservation of threatened species. Orchis patens sensu lato is a narrowly distributed tetraploid species with a disjunct distribution (i.e., Northern Italy, North Africa and the Canary
Islands), which is facing a severe decline. In this study, we evaluated levels of genetic
diversity and population structuring using 12 new nuclear microsatellite markers. Our
analyses of genetic differentiation based on multiple approaches (Structure analysis, PCA
analysis, and F-statistics using the ploidy-independent Rho-index) showed that gene flow is
low across the range of O. patens s.l., particularly in the Canary Islands. Clear differences
in allele frequencies between Italy, Algeria and the Canary Islands underlie the genetic differentiation retrieved. Our study provides support for the recognition of O. canariensis as a
sister species to O. patens and the separation of the Italian populations as a new subspecies
of O. patens. Despite the high heterozygosity values found in all populations (ranging from
0.4 to 0.7), compatible with the tetraploid status of the species, small population sizes and
reduced gene flow will be likely detrimental for the different populations in the long term,
and we recommend immediate conservation actions to counteract further fragmentation
and population decline.
Keywords Disjunct distribution · Orchids · Population genetics · Simple Sequence Repeats
(SSRs)

Résumé
Rebbas, K., Bougaham, A.F., Rebbas, N. & E. Véla (2021): Inventaire des
Orchidées de la Wilaya de Béjaïa (Petite Kabylie, Algérie) sur dix-sept années.-
J. Eur. Orch. 53 (2-4): 179-268.
Cet article représente un travail collaboratif initié il y a 17 ans (2004-2020) sur
l’inventaire, l’illustration, la cartographie et la taxonomie des Orchidées dans les
52 communes de la Wilaya de Béjaïa. Cette wilaya se situe dans le secteur de la
petite Kabylie, abritant une flore riche et diversifiée dont le recensement est
encore incomplet. Avec 44 orchidées (espèces et sous espèces) et sept hybrides
sur plus de 1000 observations uniques, cette région confirme le fort potentiel de
diversité connu dans toute la Kabylie et la Numidie. Le travail de révision
taxonomique sur les orchidées a été mené en appui à divers travaux sur les
Orchidées de l’Algérie et de l’Afrique du Nord.
Les auteurs présentent les stations d’orchidées découvertes et redécouvertes
dans la wilaya de Béjaia. Des espèces et sous-espèces d’orchidées ont été
observées, identifiées et localisées. Parmi ces espèces, Ophrys omegaifera subsp. hayekii,
Ophrys funerea, Ophrys maghrebiaca, Ophrys marmorata,
Orchis mascula subsp. maghrebiana et Orchis purpurea, ont été observées ou
confirmées pour la première fois ou seulement récemment dans la wilaya.

Ce travail a pour objet de valoriser les bio-déchets du restaurant universitaire Hasnaoua II (Tizi Ouzou) avec les déchets de palmiers du Jardin d’Essai El Hamma (Alger) et le marc de café, par la technique du compostage. La mise en place des déchets a été réalisée devant le restaurant, juste avant le confinement dû au COVID-19 le 12-03-2020 , puis se laisser composter tout seul sans suivi.
L’échantillonnage a été réalisé en novembre 2020 entre 2 compost (le non suivi mais bien équilibré en matière de carbone/azote) et l’autre compost (bien suivi et non équilibré en matière de carbone/azote) réalisé par le deuxième auteur (DA), pour objectif de réaliser des analyses physico-chimiques comparatives entre les 2 échantillons en conclusion.
A partir de ce travail, nous avons effectué des analyses de paramètres suivants : Le pH et la teneur en matière organique, teneur en carbone organique et le taux de germination.
L’échantillon 01 : compost non suivi, le pH atteint 7,57. Il contient des teneurs de carbone organique de 27.58 % ainsi 47,44% de matière organique et 74% comme taux de germination, tandis que l’échantillon 02 : compost suivi et retourné, le pH atteint 8 ,07 et des teneurs de carbone de 8 ,23% ainsi 14,6% de matière organique et 64% comme taux de germination.
La combinaison des bio-déchets et le broyats de palmiers avec le marc de café de l’échantillon 01 forment un très bon équilibre carbone /azote et par conséquence formation d’un compost riche en matière organique pour l’amendement des sols, Ainsi une réduction pour une quantité importante de déchets organiques qui lutte contre la pollution environnementale.

Mots clés : Déchets, Valorisation, Environnement, compostage, Tizi Ouzou.

Ce travail a pour objet de valoriser les bio-déchets du restaurant universitaire Hasnaoua II (Tizi Ouzou) avec les déchets de palmiers du Jardin d’Essai El Hamma (Alger) et le marc de café, par la technique du compostage. La mise en place des déchets a été réalisée devant le restaurant, juste avant le confinement dû au COVID-19 le 12-03-2020 , puis se laisser composter tout seul sans suivi.
L’échantillonnage a été réalisé en novembre 2020 entre 2 compost (le non suivi mais bien équilibré en matière de carbone/azote) et l’autre compost (bien suivi et non équilibré en matière de carbone/azote) réalisé par le deuxième auteur (DA), pour objectif de réaliser des analyses physico-chimiques comparatives entre les 2 échantillons en conclusion.
A partir de ce travail, nous avons effectué des analyses de paramètres suivants : Le pH et la teneur en matière organique, teneur en carbone organique et le taux de germination.
L’échantillon 01 : compost non suivi, le pH atteint 7,57. Il contient des teneurs de carbone organique de 27.58 % ainsi 47,44% de matière organique et 74% comme taux de germination, tandis que l’échantillon 02 : compost suivi et retourné, le pH atteint 8 ,07 et des teneurs de carbone de 8 ,23% ainsi 14,6% de matière organique et 64% comme taux de germination.
La combinaison des bio-déchets et le broyats de palmiers avec le marc de café de l’échantillon 01 forment un très bon équilibre carbone /azote et par conséquence formation d’un compost riche en matière organique pour l’amendement des sols, Ainsi une réduction pour une quantité importante de déchets organiques qui lutte contre la pollution environnementale.

Mots clés : Déchets, Valorisation, Environnement, compostage, Tizi Ouzou.

This work aims to valorize the bio-waste of the university restaurant Hasnaoua II (Tizi Ouzou) with the waste of palms of the Jardin d'Essai El Hamma (Algiers) and the coffee grounds, by the technique of composting. The placement of the waste was carried out in front of the restaurant, just before the containment due to COVID-19 on 12-03-2020, then let compost by itself without monitoring.
The sampling was carried out in November 2020 between 2 compost (the not followed but well balanced in carbon/nitrogen) and the other compost (well followed and not balanced in carbon/nitrogen) carried out by the second author (DA), for the purpose of carrying out comparative physico-chemical analyses between the 2 samples in conclusion.
From this work, we carried out analyses of the following parameters: pH and organic matter content, organic carbon content and germination rate.
The sample 01: compost not followed, the pH reaches 7,57. It contains organic carbon contents of 27.58% and 47.44% of organic matter and 74% as germination rate, while sample 02: compost followed and returned, the pH reaches 8.07 and carbon contents of 8.23% and 14.6% of organic matter and 64% as germination rate.
The combination of bio-waste and palm shreds with coffee grounds of sample 01 form a very good carbon/nitrogen balance and consequently form a compost rich in organic matter for soil amendment, thus a reduction for a significant amount of organic waste that fights against environmental pollution.

Key words: Waste, Valorization, Environment, composting, Tizi Ouzou.

This work aims to valorize the bio-waste of the university restaurant Hasnaoua II (Tizi Ouzou) with the waste of palms of the Jardin d'Essai El Hamma (Algiers) and the coffee grounds, by the technique of composting. The placement of the waste was carried out in front of the restaurant, just before the containment due to COVID-19 on 12-03-2020, then let compost by itself without monitoring.
The sampling was carried out in November 2020 between 2 compost (the not followed but well balanced in carbon/nitrogen) and the other compost (well followed and not balanced in carbon/nitrogen) carried out by the second author (DA), for the purpose of carrying out comparative physico-chemical analyses between the 2 samples in conclusion.
From this work, we carried out analyses of the following parameters: pH and organic matter content, organic carbon content and germination rate.
The sample 01: compost not followed, the pH reaches 7,57. It contains organic carbon contents of 27.58% and 47.44% of organic matter and 74% as germination rate, while sample 02: compost followed and returned, the pH reaches 8.07 and carbon contents of 8.23% and 14.6% of organic matter and 64% as germination rate.
The combination of bio-waste and palm shreds with coffee grounds of sample 01 form a very good carbon/nitrogen balance and consequently form a compost rich in organic matter for soil amendment, thus a reduction for a significant amount of organic waste that fights against environmental pollution.

Key words: Waste, Valorization, Environment, Composting, Tizi Ouzou.

Ce travail présente une synthèse préliminaire des observations inédites des orchidées effectuées entre juin et octobre 2021 au niveau de la région de Tizi Ouzou et celles de deuxième auteur (RK) effectuées entre 2010 - 2019 au parc national de Djurdjura.
L'insuffisance d'informations sur les aires de répartition des orchidées de la wilaya de Tizi Ouzou, nous laisse opter pour une méthode d'échantillonnage subjectif qui cible directement le lieu de développement des orchidées comme l'avaient déjà effectué De Belair et al. (2005) pour l'ensemble de la Numidie, Rebbas & Véla (2021) sur un transect Kabylie – Monts de Hodna et Rebbas et al. (2021) dans la wilaya de Béjaia.
Sur les stations (Ait Zikki, Azrou n’thor, Tirourda, Tikjda, parc national de Djurdjura, Ouacif, Chellata, Akfadou) qui ont été prospecté, nous avons inventorié les orchidées suivantes : Anacamptis coriophora (L.) R.M.Bateman, Pridgeon & M.W.Chase subsp. fragrans (Pollini) Bateman, Pridgeon & Chase, Anacamptis papilionacea subsp. expansa (Ten.) Amard. & Dusak, Anacamptis pyramidalis (L.) Rich. subsp. pyramidalis, Androrchis mascula (L.) D.Tyteca & E.Klein subsp. maghrebiana (B.Baumann & H.Baumann) W.Foelsche & Jakely, Androrchis olbiensis (Reut. ex Gren.) D.Tyteca & E.Klein, Androrchis pauciflora (Ten.) D.Tyteca & E.Klein subsp. laeta (Steinh.) Véla, Rebbas & R.Martin, Cephalanthera longifolia (L.) Fritsch, Cypripedium calceolus L., Dactylorhiza elata (s.l.), Dactylorhiza maculata subsp. battandieri (Raynaud) H.Baumann & Künkele, Epipactis helleborine (L.) Crantz, Himantoglossum hircinum (L.) Spreng. subsp. hircinum, Ophrys apifera Huds., Ophrys atlantica Munby subsp. atlantica, Ophrys battandieri E.G.Camus, P.Bergon & A.Camus, Ophrys fusca s.l., Ophrys lutea Cavanilles subsp. lutea, Ophrys numida Devillers-Tersch. & Devillers, Ophrys omegaifera subsp. hayekii (H. Fleischm. & Soo) Kreutz, Ophrys speculum Link subsp. speculum, Ophrys tenthredinifera subsp. ficalhoana (J.A.Guim.) M.R.Lowe & D.Tyteca, Orchis anthropophora (L.) All., Orchis italica Poir., Spiranthes spiralis (L.) Chevall.
Cette contribution à cet inventaire ne s'arrête pas là, il s'agira d’élargir nos prospections orchidologiques sur d’autres stations (communes) de la wilaya de Tizi Ouzou durant l'année 2022 pour évaluer leurs états de conservation.

Mots clés : Orchidées, inventaire, écologie, endémisme, conservation, Tizi Ouzou.

Ce travail présente une synthèse préliminaire des observations inédites des orchidées effectuées entre juin et octobre 2021 au niveau de la région de Tizi Ouzou et celles de deuxième auteur (RK) effectuées entre 2010 - 2019 au parc national de Djurdjura.
L'insuffisance d'informations sur les aires de répartition des orchidées de la wilaya de Tizi Ouzou, nous laisse opter pour une méthode d'échantillonnage subjectif qui cible directement le lieu de développement des orchidées comme l'avaient déjà effectué De Belair et al. (2005) pour l'ensemble de la Numidie, Rebbas & Véla (2021) sur un transect Kabylie – Monts de Hodna et Rebbas et al. (2021) dans la wilaya de Béjaia.
Sur les stations (Ait Zikki, Azrou n’thor, Tirourda, Tikjda, parc national de Djurdjura, Ouacif, Chellata, Akfadou) qui ont été prospecté, nous avons inventorié les orchidées suivantes : Anacamptis coriophora (L.) R.M.Bateman, Pridgeon & M.W.Chase subsp. fragrans (Pollini) Bateman, Pridgeon & Chase, Anacamptis papilionacea subsp. expansa (Ten.) Amard. & Dusak, Anacamptis pyramidalis (L.) Rich. subsp. pyramidalis, Androrchis mascula (L.) D.Tyteca & E.Klein subsp. maghrebiana (B.Baumann & H.Baumann) W.Foelsche & Jakely, Androrchis olbiensis (Reut. ex Gren.) D.Tyteca & E.Klein, Androrchis pauciflora (Ten.) D.Tyteca & E.Klein subsp. laeta (Steinh.) Véla, Rebbas & R.Martin, Cephalanthera longifolia (L.) Fritsch, Cypripedium calceolus L., Dactylorhiza elata (s.l.), Dactylorhiza maculata subsp. battandieri (Raynaud) H.Baumann & Künkele, Epipactis helleborine (L.) Crantz, Himantoglossum hircinum (L.) Spreng. subsp. hircinum, Ophrys apifera Huds., Ophrys atlantica Munby subsp. atlantica, Ophrys battandieri E.G.Camus, P.Bergon & A.Camus, Ophrys fusca s.l., Ophrys lutea Cavanilles subsp. lutea, Ophrys numida Devillers-Tersch. & Devillers, Ophrys omegaifera subsp. hayekii (H. Fleischm. & Soo) Kreutz, Ophrys speculum Link subsp. speculum, Ophrys tenthredinifera subsp. ficalhoana (J.A.Guim.) M.R.Lowe & D.Tyteca, Orchis anthropophora (L.) All., Orchis italica Poir., Spiranthes spiralis (L.) Chevall.
Cette contribution à cet inventaire ne s'arrête pas là, il s'agira d’élargir nos prospections orchidologiques sur d’autres stations (communes) de la wilaya de Tizi Ouzou durant l'année 2022 pour évaluer leurs états de conservation.

Mots clés : Orchidées, inventaire, écologie, endémisme, conservation, Tizi Ouzou.

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Influences des extraits de Asphodeline lutea sur la mortalité de Culiseta

Les auteurs signalent la découverte récente en Algérie d’une spermatophyte de la famille des
Pedaliacées. Le sésame, Sesamum indicum L. (Syn. S. orientale L.), observé pour la première
fois dans la région de Bordj Bou Arreridj.
Mots clés : Sesamum indicum L., découverte, Bordj Bou Arreridj, Algérie, Afrique du nord.

L’auteur signale la découverte à Akbou (Béjaia, NE-Algérie) d’ibicelle
jaune originaire des zones semi‐désertiques d’Amérique du Sud.
Mots clés: Ibicella lutea, découverte, Algérie, Afrique du Nord.

In this study, the antioxidant and antimicrobial activities of Echinophora spinosa were
investigated. Antimicrobial activity of methanol extracts obtained from Echinophora
spinosa was examined using the disc diffusion method. Antioxidant activity of the methanol
extracts was examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical
scavenging test. Methanol extract of ripe fruits of E. spinosa showed highest total phenolic
content (69.17 ± 1.2 µg GAE/mg extract) and the major flavonoid contents (12.122 ± 0.44 µg
QE/mg extract) was found in leaves of the plant. In disk diffusion antimicrobial assay, E.
spinosa manifested broad spectrum of activity. The largest capacity to neutralize DPPH
radicals was found for ripe fruits methanol extract of E. spinosa plant. The results shows that
the various parts of E. spinosa extracts promising antioxidant and antimicrobial activities have
potential bioactivities due to high content of phenolic and flavonoid compounds.

Discovery of Epipactis microphylla (Orchidaceae) in Djebel Babor (north-eastern Algeria), a
new orchid for North Africa. — Epipactis microphylla is a European-Hyrcanian, forest mycoheterotrophic species, widespread in temperate and submeridional zones, from Andalusia to the
Caspian Sea. This orchid has been discovered in Djebel Babor, in northeastern Algeria, in a
mixed undergrowth of Cedars, Zean oaks and Holm oaks, where it is to date the only known
resort in North Africa. This discovery confirms the interest of Babors’ Kabylia in general and
the new Babor-Tababort National Park in particular.
Key words: protected areas, rare species, peripherical distribution, important plant area, partial
mycoheterotrophy

Cephalanthera rubra (L.) Rich (Orchidaceae) est une espèce eurasiatique tempérée et
subméditerranéenne ; de l’Atlantique à la mer, sur substrats calcaires à un peu acides.
Cette orchidée a été découverte au Babor, dans le nord-est de l’Algérie. Les notes écologiques
sont également fournies. Cette découverte enrichira l’orchidoflore Algérienne en général et
celle de la Kabylie des Babors en particulier.
Mots clés : flore, floristique, orchidée, Babor, Afrique du Nord, Algérie.

Les auteurs signalent la découverte à Akbou (Béjaia, NE-Algérie) et à Tissemsilt (W-Algérie)
d’ibicelle jaune (Martyniaceae) originaire des zones semi‐désertiques d’Amérique du Sud.
Mots clés: Ibicella lutea, découverte, Algérie, Afrique du Nord

Ce document est un travail de synthèse des données récentes concernant les Orchidées du nord-est de l’Algérie.
Les régions concernées sont la Kabylie au sens large et la Numidie historique algérienne avec ses montagnes
adjacentes du Hodna et de l’Aurès. Ce sont des territoires très divers sur le plan géographique (du littoral jusqu’à + de
2000m d’altitude), bioclimatique (de l’étage semi-aride à l’étage per-humide) et lithologique (alluvions, calcaires,
dolomies, argiles, schistes, grès, granites, etc.).
Toutes les données publiées à partir de 2005 ont été synthétisées et beaucoup de données inédites observées à
partir de 2002 ont été rassemblées grâce à une quinzaine de collaborateurs. Malgré cela de nombreux secteurs sont
encore vierges de prospection et devront être investigués à l’avenir.
C’est donc 55 espèces qui ont été illustrées et cartographiées. 8 autres sont connues historiquement mais n’ont pas
encore été retrouvées. Enfin une dernière a été signalée par le passé par erreur. Les principaux synonymes sont
rappelés. Le choix taxonomique s’inspire d’un travail récent similaire effectué sur les Orchidées de Tunisie, à quelques
réarrangement près au niveau des genres. Une combinaison nomenclaturale nouvelle a été créée pour l’occasion
(Androrchis pauciflora subsp. laeta).
Un appel à contribution est lancé auprès de tous les botanistes professionnels et amateurs algériens afin de
poursuivre le travail à l’échelle de tout le Tell algérien.

Abstract
The essential oil was obtained by separate hydrodistillation from the aerial parts of Carduncellus pinnatus (Desf.) DC.
(Asteraceae) and was analyzed by means of gas chromatography (GC) and gas chromatography–mass spectrometry
(GC-MS). Hundred and four compounds were detected, accounting for 95.29 % of the total oil, which is characterized
by a high content of non-terpene derivatives (33.39%) and oxygenated sesquiterpenes (32.03%). The main constituents
of the essential oil from the aerial parts were caryophyllene oxide (23.12%), (E)-2-hexenal (8.35%), (E)-β-ionone
(6.01%), (E)-geranylacetone (2.96%), and nonanal (2.65%). Additionally, β-Carotene-linoleic acid test system, DPPH
free radical scavenging assay and ABTS cation radical scavenging assay were tested for the study of antioxidant
activity of plant methanol extract and essential oil. The MeOH extract of aerial parts exhibited a strong antioxidant
activity evidenced by the ABTS cation radical scavenging assay, with IC50 values of 44.509 ± 1.149 µg/ml. In the DPPH
test the IC50 values were 61.147 ± 0.924µg/ml, while in β-carotene-linoleic acid test system, the IC50 values were
153.744 ± 3.39µg/ml. Essential oil extract of aerial parts showed 81.80 ℅ inhibition against butyrylcholinesterase at
200 µg/mL. The highest antibacterial activity was evidenced for the aerial parts MeOH extracts, with inhibition zone
medium diameters of 18.66±1.00 mm against Escherichiacoli ATCC25922 at 16 mg/ml and of and 18.00±1.15mm
against Micrococcus luteus ATCC533 at 16 mg/ml. This is the first report about the potential for food and
pharmaceutical industry due to the composition and the biological activities of Carduncellus pinnatus essential oils
growing in Algeria.
Keywords: Carduncellus pinnatus, essential oil, anticholinesterase, antibacterial, antioxidant
activities, GC-MS.

To control vinegar flies, insecticide trials were carried out using some extracts of Cleome arabica (Capparidaceae), a
medicinal plant used for the treatment of inflammation, pyrexia and pain.
In this study, we evaluate the direct and delayed effect of the ethanolic plant extract on the vinegar fly Dorsophila
melanogaster, the excellent laboratory model. The treatment was applied on 2nd larvae stage (L2), by ingestion.
Several concentrations were used to determined fly mortality and sublethal concentration (0.5 μg / ml) was used to
studied sexual behaviour of adults.
We record 50% morality rates after 15 days treatment and a disturbance of fly development. The results also show that
C. arabica ethanolic extract has a negative effect on the Drosophila sexual behaviour.
Keywords: Cleome arabica, Drosophila melanogaster, mortality, sexual behavior

The ecodendrological study, on 35 plots were sampled in the Alep pine formation of the Ain
Hamda Tizi N’bechar forest at the rate of one plot per station and in each plot a
Phytoecological floristic survey was carried out. Concerning the species of the floral
procession of the pine forest, there are 19 medicinal species, belonging to 13 families and 20
genera. This medicinal potential must be protected and preserved; it is in this context that this
study is based on the knowledge of the factors which influence the distribution and the
distribution of these species within the framework of a good rational management in support
of the local and sustainable development of the local population through valuation

The phytochemical and biological properties of Ononis alba Poir
L. (Fabaceae) were investigated for the first time in this study.
The chemical composition of the essential oil obtained from the
aerial parts was analysed by GC-MS. The phenolic contents of
extracts obtained with different solvents were determined by the
Folin-Ciocalteu assay and the antioxidant activity was evaluated
through DPPH and CUPRAC methods. The inhibitory potential of
these extracts was evaluated on a-amylase and a-glucosidase,
whereas the antimicrobial effect was verified against some bacteria and fungi through the well diffusion method. Ketones and
carboxylic acids were the main essential oil constituents. The
highest total phenolic and flavonoid content as well as the best
antioxidant capacity were noticed on the n-butanol extract. All
the extracts showed a greater efficiency than acarbose in the
inhibition of a-amylase. On the other hand, they demonstrated a
mild inhibition effect against Staphylococcus aureus and
Fusarium oxysporum.

Ethnopharmacological relevance: Ethnoveterinary medicine is vastly under-documented, especially in North
Africa, where livestock care is increasingly medicalised. Despite evidence of the interdependence of ethnoveterinary practices and ethnomedicine for human care, the overlap between these two systems and the possible
drivers of similarity are rarely addressed in ethnopharmacological literature. Here, we present the first quantitative comparison of remedies used to treat human and animal health among a pastoral society.
Aims: This study aims to document the plants and other materials used in ethnoveterinary medicine among
nomadic herders in the Algerian steppe. We calculate the overlap with remedies used for human health and
evaluate some of the possible drivers of similarities between the two interlinked medical systems.
Methods: The field study was conducted in spring 2018 with 201 local knowledge holders in five provinces in the
central part of the Algerian steppe. Forty-six camps and ten weekly animal markets were visited. After obtaining
prior informed consent, data was collected through structured interviews. Anonymous sociodemographic information was collected along with veterinary use data. Plant specimens were acquired, identified and deposited
in the Botanical Laboratory Herbarium of the University of Tiaret. Ethnobotanical information was structured in
use reports and therapeutic applications organised into 13 simple categories. Plants used in ethnoveterinary
medicine were compared to those used in human health care using a Chi square test, and ethnoveterinary use
was predicted using a generalised linear model with use for human care and plant family as predictive variables.
Logistic regressions were also used to test if any specific medicinal application predicts shared use in human and
veterinary medicine.
Results: Sixty-six plant species from 32 botanical families and ten non-vegetable remedies were documented.
Plants from the Lamiaceae, Asteraceae, Fabaceae and Apiaceae families were most commonly used. The plants
most often cited by interviewees are all harvested from the wild (Echinops spinosissimus Turra, Atriplex halimus L.,
Artemisia campestris L., Juniperus phoenicea L. and Peganum harmala L.). Leaves are the most commonly used plant
part and decoction is the most common preparation method. There are important similarities between remedies
used to treat humans and other animals: two thirds of the remedies used in ethnoveterinary medicine are also
used in human health care, and these represent half of the human health treatments. Use for human health and
plant family both predict the use of a medicinal plant in veterinary medicine, but no correlation is found regarding specific therapeutic applications.
Conclusions: Traditional veterinary knowledge is still key to sheep and goat herders in the Algerian steppe, but a
knowledge transmission gap seems to exist between older and younger generations, and ethnoveterinary
practices may disappear in the near future. Treatments for human and animal care overlap to a large extent, and
a causal relationship possibly exists for, at least, some of them. However, overall a smaller number of remedies

Analyse de l'état de la phytodiversité dans les zones steppiques, cas des monts de Dreat , M'sila

Effet toxiques des extraits des plantes sahariennes sur la mortalité et le comportement de Drosophila

Evaluation des extraits aqueux de Cleome arabica sur la reproduction et la fertilité de Drosophila

Larvicidal effect of Physalis acutifolius on Culiseta

Perturbation du comportement alimentaire des larves de Drosophila par les extraits Cleome arabica

HPLC analysis of phenolic acids and flavonoids in ethyl acetate extrat of Lepidium

Effets diffère de Ruta chalepensis sur le comportement de Drosoplhila

Dépôt des spécimens de plantes dans d'herbier de l'ENSA Alger 2019

Inventaire systématique et diversité biologique de Culicidae (Diptera: Nematocera) dans l’oasis d’Elkantara -Biskra- (Algérie)

Effets toxiques direct de l’extrait éthanolique de Cleome arabica (Capparidaceae) sur la
mortalité et le développement de Drosophila melanogaster (Diptera ; Drosophilidae) »

Inventaire des plantes messicoles des champs de culture de Hammam Dalaa (M'Sila, Algérie).

Inventaire des plantes à pollen allergisant dans la région de Maâdid (M’Sila, Algérie).

Discovery of Centaurea hyalolepis Boiss. (Asteraceae) in the region of Djelfa
(Algeria)
Mots clés: Asteraceae, Centaurea hyalolepis, découverte, Djelfa.

Espèce perdue de vue depuis plus de 80 ans en Afrique du Nord, Allium
scaberrimum J. Serres (Amaryllidaceae) a été observé dans plusieurs localités du nord de
l’Algérie, recoupant les trois régions historiques de présence dans le pays. Malgré sa persistance
à long terme, la raréfaction de son habitat exclusif justifie son statut actuel d’espèce menacée.
Cela devrait encourager à une meilleure connaissance de ses populations sur le terrain ainsi qu’à
des actions de conservation ex situ et de gestion puis de renforcement ou réintroduction in situ.
Mots clés. – Dayat el Ferd, espèce menacée, massif de l’Aurès, monts du Hodna,
plante messicole, zone importante pour les plantes.

Christella dentata (= Cylosorus dentatus, Thelypteridaceae, Pteridophyta), native to the tropical and subtropical regions of Africa and Asia, has been observed between Jijel and Bejaia in
northeastern Algeria. Distribution, ecological notes and comparison with related taxa as
Cyclosorus interruptus (Dryopteris gongylodes p.p.) are also provided. This is the first report
of this species in North Africa.
Key words: Pteridophytes, biodiversity survey, flora of Algeria, North Africa, Important Plant Area.

In the highlands of Bordj Bou Arreridj in Northeast Algeria, traditional knowledge about medicinal plants is still
put to use, but has been poorly explored by researchers. In view of its natural and cultural richness and the socioeconomic context, this area is an ideal location for the study of aromatic and medicinal plants and their traditional uses. The aim of this ethnobotanical study was to shed light on how medicinal plants are used in
traditional phytotherapy in the region of Bordj Bou Arreridj. Over a two-year period (2014–2016), herbalists
practicing phytotherapy in the region were surveyed, and asked to provide information about themselves and the
plants they used (vernacular name, mode of preparation, parts used and toxicity). The data was analyzed in
terms of the Use-Value of species (UV), Medicinal Informant Agreement Ratios (Med. IARs), Fidelity Level (FL)
and the Informant Consensus Factor (ICF). Among the 51 herbalists interviewed, men dominated the practice of
traditional phytotherapy. The majority of these herbalists, whose ages varied between 46 and 65, were illiterate.
During the survey, 83 species of medicinal plants were identified. They belonged to 66 genera and 37 botanical
families, among which Lamiaceae were the most used. The herbalists most commonly used plant leaves, and for
the most part, preferred to prepare the remedy in the form of infusions. Origanum vulgare subsp. glandulosum
(Desf.) Ietsw., Rosmarinus eriocalyx Jord.& Fourr. and Allium cepa L. were the plants with the most important UV.
Thirty-eight species had maximum values for the Med IARs index and digestive diseases were those with the
highest ICF value. Interviewees indicated 10 taxa possessing toxicity but did not indicate the toxicity limits of
each plant. Taxonomic analysis allowed the authors to recognize several taxa having changed systematic positions and/or nomenclature. The chorological analysis led the authors to distinguish 6 endemic taxa and 13 taxa
that were not native to the country. The importance of this study lies in the originality of the information
obtained, which must be preserved and used by researchers in pharmacology and other fields. Particular interest
should be devoted to the taxonomic and chorological aspects of medicinal plants in order to avoid common
serious errors in this kind of research.

Cleome arabica is a spontaneous plant in arid zones,
toxic and has hallucinogenic effects. In this work, we seek to
evaluate the direct and delayed effect of the aqueous extracts
on the vinegar fly Dorsophila melanogaster, laboratory model
par excellence. Treatment is by ingestion on second instar
larvae (L2). The results show that mortality rates can reach
90% after 15 days of treatment as we recorded a disturbance
of fly development. Observation of sexual behavior in
treatment-emergent adults indicates that C. arabica acts
negatively on the course of the different sequences leading to
mating in Drosophila.
Keywords: courtship, mating, Saharan plant, toxicity,
Vinegar fly

L’effet de l'extrait aqueux de Ruta chalepensis L.., plante spontanée du littoral
algérien à des concentrations (8g/l, 18,44g/l et 33,2g/l), a été observé sur les larves de
quatrième stade du Culiseta longiareolata (Macquart) sous des conditions contrôlées.
Les résultats montrent que le taux de mortalité augmente en fonction de la
concentration de l'extrait et du temps. Le taux de mortalité le plus important (100%) a
été obtenu après 3 jours de traitement pour la concentration la plus élevée, avec des
concentrations létales CL50% et CL90% sont 8,82g/l et 14,31g/l, respectivement. Alors
que le temps létal, le minimum (0,66 jours) a été enregistré avec la dose 33,2g/l.
Certains composés de Ruta chalepensis pourraient être utiles dans l'élaboration ou la
synthèse de bio-insecticides, dans le cadre d'une lutte intégrée contre les moustiques

The essential oils obtained by hydrodistillation of aerial parts of Centaurea dimorpha Viv.
were analyzed by means of gas chromatography-mass spectrometry (GC-MS). The main constituents
were Caryophyllene oxide (09.88%), limonene (5.73%), tetradecanoic acid (5.68%), spathulenol
(5.44%), methyl hexadecanoate (4.45%), and α-pinene (03.08%). To our best knowledge this is the first
study of essential oils from the aerial parts of Centaurea dimorpha growing in Algeria. Moreover the
essential oil and methanolic extract were screened for their possible in vitro antioxidant activity by
DPPH free radical-scavenging test. The findings showed that the percentage inhibition is 10.67 and
77.01 respectively at a concentration of 1mole/L.
Keywords: Centaurea dimorpha; Essential Oi; GC-MS; aerial parts; Antioxidant activity

Discovery of Cypripedium calceolus (Orchidaceae) in Djurdjura (Algeria), news for North
Africa. — Cypripedium calceolus L. (Orchidaceae) native to Eurasia is considered one of the
flagship plant species of nature conservation. Because of its wide range, this species could be
considered a taxon of minor concern, near threatened, vulnerable, endangered or critically
endangered.
This orchid was discovered in the Djurdjura, in north-central Algeria. Ecological notes are also
provided. This is the first report of this species in North Africa. This discovery will enrich the
Algerian orchid in general and that of Kabylia in particular.
Key words: flora, floristics, Orchid, North Africa.

Rebbas K. - Membre collaborateur et intervenant dans un projet de la Flore du Maghreb -2018
http://www.ville-ge.ch/cjb/flore/html/index.html

A N T I O X I D A N T A C T I V I T Y , PHENOLIC A N D F L A V O N O I D C O N T E N T S OF A L G E R I A N SPECIE OF I N U L A

Dépôt des spécimens de plantes dans d'herbier de l'ENSA Alger

Conserving wild plants in the south and east Mediterranean region - contribution par des photos K. Rebbas

Phytodiversité de l'oued Bousselam, Sétif

Flore médicinale de Maadid, M'sila

Inventaire et cartographie des Dactylohriza

Analyse de la biodiversité de Maadid ( Msila)

Larvicidal effect of Physalis acutifolia on Culiseta longiareolata
(Diptera ; Culicidae)

Flore médicinale de la région de Maadid: inventaire et enquete ethnobotanique

Flore medicinale de la région de Maadid

Plantes d'intérêt médicinal - sud Bouira

Potentiel floristique, écologique et médicinal de Djebel Djedoug (M’sila).

Evaluation des activités larvicides d’extrait aqueux des feuilles du la plante Solanum nigrum sur les larves de Culiseta longiareolata (Diptera, Culicidae).

Bioactivité des extraits foliaires de Ruta chalepensis L. (Rutaceae) sur la mortalité des adultes de Blattella germanica, (Dictyoptera, Blattellidae).

Orchidées d’Algérie : inventaire, cartographie et conservation.

Etude ethno vétérinaire des plantes médicinales de deux régions : Bordj Bou Arreridj et Sétif (Algérie).

Le Professeur Pierre Quézel à qui nous avons présenté le projet en juin 2015 quelques mois avant sa disparition, nous avait accueillis avec toute sa bienveillance et était très enthousiaste de savoir que sa flore pourrait être utile à une nouvelle génération. Il avait soutenu cette initiative et nous avait donné son autorisation en tant qu'auteur. Il avait mis également à notre disposition les dessins originaux de la Flore d'Afrique du Nord qui seront scannés et conservés aux CJB. Ce travail lui est donc entièrement dédié.

Physalis acutifolia (Solanaceae), native to the southwestern United States, California Texas,
and northern Mexico, has been observed in many localities in the M'Sila region, central north
Algeria. Distribution and ecological notes are also provided. This is the first report of this
species in N Africa.
Key words: xenophytes, weeds, North Africa, medicinal plants

Les 140 enquêtes menées auprès des différents intervenants dans la gestion des déchets de santé
(publics et privés) et des collecteurs de déchets de l'Assemblée populaire communale (APC) à travers les
quatorze daïras de la wilaya ont confirmé l'existence de grandes quantités de déchets de soins jetés directement
dans des sites d'enfouissement incontrôlés comme s'ils étaient des ordures ordinaires en raison de l'absence de
mesures répressives par les services concernés qui pourraient mettre fin à ces dépassements menaçant la santé
publique, la biodiversité et l'environnement en général.
Mots clés : Déchets d’activité de soins, Environnement, Biodiversité, Santé publique

Le présent travail porte sur la gestion des déchets ménagers de la ville de M'sila et l'objectif principal
est d'arriver à déduire les déterminants clés de la bonne gestion des déchets ménagers dans cette ville.
Pour ce faire, nous nous sommes rapprochés de la population des trois quartiers (la cité 06, 1000 logements et le
pôle urbain), choisis en tenant compte des disparités entre le différent type d'habitats et du niveau de vie de la
population à interroger. Le questionnaire a été administré personnellement, sur les lieux, de porte à porte, auprès
de 210 ménages, à raison de 70 ménages par quartier.
La gestion des déchets ménagers dans la ville de M'sila, rencontre beaucoup de problèmes, dont le manque de
matériel de collecte de service municipale, conjointement au non-respect de la population des horaires de sortie
des déchets et du mode d'évacuation, aboutissant à une synchronisation de la collecte avec la sortie des déchets
par les habitants, en plus du changement de l'emballage des déchets et de leur rejet n'importe où par une certaine
proportion des ménages interrogés. Ceci mène à une accumulation des déchets dans les quartiers.
Mots clés : Déchets ménagers, Environnement, Biodiversité, Santé publique.

La région d’Alger a un problème aigu de pollution atmosphérique urbaine et industrielle. Les
différentes sources de pollution sont liées aux unités industrielles, urbanisation anarchique et à l’extension du
parc automobile.
L’objectif de cette présente étude d’utiliser les lichens comme bio indicateurs de la pollution atmosphérique dans
la région Est d’Alger. La méthode utilisée est celle de l’Indice de Pureté Atmosphérique. Elle consisté à traduire
la richesse floristique des lichens en une expression mathématique unique, une fois ses indices ont été calculé
pour les 32 stations choisies dans la zone d’étude, ses indices ont été groupé en classes pour donner trois zones
d’iso pollution correspondant à différent niveaux de pollution donnant à la fin la carte de pollution
atmosphérique globale de la région est d’Alger.
Nous avons inventorié 24 lichens appartenant à neuf familles. A chaque une des espèces recensées nous avons
réalisé une carte de répartition de l’espèce et qui nous permet de surveiller la pollution atmosphérique de la zone
d’étude.
Mots clés : Alger, inventaire, cartographie, lichens, bio indicateurs de la pollution de l’air

The aim of this study was to isolate the phytoconstituents and evaluate the
biological activities of Berberis hispanica. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from B. hispanica. The structures of
the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition,
antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some
fractions and isolated compounds were evaluated. The extracts of B. hispanica
were evaluated against six bacterial strains and exhibited the highest activity
against Klebsiella pneumonia (15 mm at 100 mg/mL). Fraction T36 (IC50<5 μg/
mL) from the n-butanol extract displayed higher radical scavenging activity
than butylated hydroxytoluene. The isolated compounds were evaluated for
their anti-proliferative effects against human cervical adenocarcinoma (HeLa)
cell line by real time cell analyzer assay and tamarixetin exhibited the
remarkable effect (IC50<50 μg/mL) on HeLa cells. This study supports the
documented medicinal effects of B. hispanica

The aim of this study was to identify and quantify the phenolic compounds of the Ethyl
acetate extract from Lepidium draba using HPLC-TOF/MS; and to investigate the antioxidant potencies. In
this work, we have found that the Ethyl acetate soluble fraction of L. draba is a good source of antioxidant,
which is the result of its phenolic acids and flavonoids. Five methods were conducted, to determine the antioxidant
components of the Ethyl acetate extract: DPPH, ABTS, CUPRAC, Reducing power assay and Metal chelating
assay. Nineteen compounds from which 13 phenolic acids and 6 flavonoids known for their pharmacological
properties were identified by using HPLC-TOF/MS technique: Gallic acid, Gentisic acid, Chlorogenic acid, 4-
Hydroxy benzoic acid, Protocatechuic acid, Caffeic acid, Vanillic acid, 4-hydroxybenzaldehyde, p-coumaric
acid, Chicoric acid, Ferulic acid, Rosmarinic acid, Salicylic acid, Catechin, Rutin, Hesperidin, Apigenin-7-
glucoside, Quercetin, and Kaempferol. All These compounds were identified and quantified in Lepidium draba
for the first time. The IC
50 and A0.50 of the Ethyl acetate extract were 305.69±1.22, 39.42±0.45, 152.27±0.38
and 183.83±2.47 μg/mL in DPPH, ABTS, Metal chelating assay, and CUPRAC. However, the results indicate
that L. draba is a good source of natural antioxidants.
Key words: Lepidium draba, HPLC-TOF/MS, phenolic acids, antioxidant activity.

Les moustiques ont un rôle épidémiologique en tant que vecteur de plusieurs agents
pathogènes capables d’affecter l’homme. L’inventaire des Culicidés durant la période de
janvier à mai 2017 nous a permis de connaître leurs répartitions spatio-temporelles dans
plusieurs stations de la région de Bousaâda (M’Sila). Les échantillonnages larvaires de
moustiques ont été effectuées par d’une louche, dans les milieux urbain et rurale dans quatre
localités situées dans différentes altitudes.
Les résultats d’identification montrent l’existence de 11 espèces dans la région étudiée
(Culiseta longiareolata, Culiseta subochrea, Culex pipiens, Culex deserticola, Culex theileri,
Culex laticinctus, Culex brumpti, Culex perexiguus, Anopheles cinereus, Anopheles
multicolor, Uranotaenia unguiculata). Cet inventaire est complété par une analyse physicochimique de l’eau pour étudier la corrélation entre la densité larvaire des moustiques et la
qualité de gîte.
Mots clés : Culicidae, inventaire, systématique, analyse physico-chimique.

Nicotiana glauca (N. glauca) (Solanaceae) is an ornamental plant. It has insecticidal properties. However, these uses cannot compensate for the
overall negative impacts of this plant. This study was aimed to evaluate the larvicidal effect of the aqueous extract of N. glauca leaves on the 4th stage
larva of Culiseta longiareolata (Cs. longireolata). N. glauca leaves were harvested, dried and powdered using an electric mill. Hot aqueous extraction
was done with 150 g powdered leaf in 1000 mldistilled water. The dry extract was partitioned in various beakerswith 20 larvae, and the three
concentrations 14.85 g / l, 25.66 g / l and 62.4 g / l were determined. The preliminary results showed that used extract has a high efficiency compared
to natural substances of plant or microbial origin. This efficiency is expressed by the calculated toxicological parameters which are successively LC50
and LC90, with 26.87 and 55.14g/l, As for lethal times 0.46 and 1 days respectively. N. glauca can be considered among the plants with important
insecticidal effects in biological control against mosquitoes. Preliminary toxicity tests on Cs. longireolata have confirmed its toxicity for these
vectors.
Keywords: aqueous extract, plant, mosquito, toxicological parameters

This taxon is considered here stricto sensu, i.e. excluding "O. algarvensis" considered here in synonymy
under O. omegaifara subsp. hayekii (= O. mirabilis). Both subspecies can grow together (western Baetic
Mountains in southern Spain, Trara Mountain in northwestern Algeria) and then hybridize with
intermediate forms

Romulea battandieri Bég. is considered a synonym of the widespread Romulea columnae Sebast. &
Mauri by WCSP (2017), however participating experts at the North Africa monocotyledon assessment
review workshop (Rabat, 2016) did not agree with WCSP and considered this a valid species following
Maire (1959) and Dobignard and Chatelain (2010).

According to Martin et al. (2015), Orchis laeta is considered here including coloured forms sometimes
called Orchis x blidana as hypothetic hybrid swarms O. laeta x O. olbiensis.

This taxon is considered here lato sensu, i.e. including "O. algarvensis", like the Sicilian "O. mirabilis",
within the natural variability of O. omegaifera subsp. hayekii (typified from Tunisia) thanks to the
abundant populations discovered recently throughout northern Algeria. This subspecies can grow
together with O. omegaifera subsp. dyris (native to the western Baetic Mountains in southern Spain and
Trara Mountains in northwestern Algeria) and then hybridise with intermediate forms (E.Véla pers.
comm. 2018). Taxonomy adopted here is in conformity with Martin et al. (2015).

This orchid is endemic to the Mediterranean region, where it is only known from a few localities in
northern Algeria. Despite its restricted distribution, it is listed as Least Concern because it does not face
any major threats and occurs in protected areas and in inaccessible habitats.

Les auteurs présentent les résultats de plus de 5 années d’observations
des orchidées de l’Atlas tellien occidental de Tiaret (Algérie). Ces taxons ont été inventoriés
au niveau régional, selon un échantillonnage subjectif, et les conditions écologiques au niveau
de chacune des stations recueillies. L’identification des taxons a été faite grâce à différentes
flores, avec une conception taxonomique basée sur les critères morphologiques utilisant les
«morpho-espèces». Vingt taxons appartenant à 7 genres différents ont été identifiés, dominés
par les Ophrys et les Anacamptis. De nouvelles informations concernant la biogéographie de ces
taxons sont apportées et plusieurs difficultés taxonomiques rencontrées sont soulignées. Cette
étude a permis de comparer les données locales avec celles figurant dans quelques publications
portant sur les orchidées d’Algérie.
Mots clés. – Orchidaceae, Anacamptis, Ophrys, Tiaret (Algérie), Djebel Guezoul,
inventaire, morpho-espèce.

Compositional study, antibacteriol and antioxydant potential of Lepidium draba

Studies on the antioxydant activity of different solvent extracts of Ammi visnaga , Algeria

Utilisation d'une plante toxique Cleome arabica contre Culiseta longiareolata

Toxicité de deux extraits de deux plantes sahariennes Peganum harmala et Citrullus colocynthus sur les larves de Culiseta

Etude ethnobotanique des plantes utilisées en médecine traditionnelle dans la région
de Ben S’rour (M’sila)

Flore et Plantes médicinales du Djebel Djedoug (M’Sila-Algérie)

Découverte de Christella dentata (= Cylosorus dentatus, Thelypteridaceae, Pteridophyta) en Afrique du Nord
et révision du genre Cyclosurus s.l. en Méditerranée occidentale

Flore médicinale de la région du Djebel Bou Taleb (Algérie)

Contribution à l’étude ethnobotanique des plantes médicinales de Bordj Ghedir
(Bordj Bou Arreridj, Algérie)

Programme seminaire internationale 2017 - K. Rebbas

INVENTAIRE FLORISTIQUE SUR UN TRANSECT HAMMAM DALAA - CHOTT EL HODNA (M’SILA-ALGERIE).

BIODIVERSITE DES ORCHIDEES DE LA REGION NORD DE BOUIRA (REGION CENTRE D’ALGERIE).

ETUDES FLORISTIQUE ET ETHNOBOTANIQUE DU MASSIF DE MAADID (M’SILA, ALGERIE).

EVALUATION IN VITRO DU POUVOIR ANTIMICROBIEN ET ANTIOXYDANT DES HUILES ESSENTIELLES DES ZESTES DE FRUITS DE CITRONNIER (CITRUS LIMON (L.) BURM. F.) CULTIVES DANS LA REGION D’OUM EL BOUAGHI.

COMPARAISON DE L’EFFET ANTIBACTÉRIEN DES POLYPHÉNOLS TOTAUX ISSUS DES MARGINES DE L’EST ALGÉRIEN.

ETUDE ETHNOBOTANIQUE DES PLANTES MEDICINALES D’UNE ZONE SUD DU DJURDJURA (BOUIRA, ALGERIE).

DIVERSITY IN CHEMICAL COMPOSITIONS OF ESSENTIAL OIL AND SUPERCRITICAL EXTRACTS OF TEUCRIUM POLIUM L. FROM BORDJ BOU ARRERIDJ (SEMI-ARID ZONE IN NORTHEAST OF ALGERIA).

Examinateur des mémoires de Masters

Séminaire International sur : Phytodiversité et Plantes
d’intérêt écologique et économique en Algérie

Cette étude vise la connaissance et la recherche des plantes endémiques,
rares et menacées de l'atlas tellien occidental de Tiaret.

Dans le présent travail nous mettons en exergue la diversité de la flore des milieux forestiers et préforestiers
échantillonnées lors de nos recherches dans l’Atlas tellien occidental de Tiaret. Nous nous sommes basés dans cet
inventaire sur les 278 relevés floristiques réalisés à travers les massifs forestiers de cette région, ainsi que d’autres
observations. Nous présentons cet inventaire sous la forme d’un catalogue floristique actualisé sur le plan nomenclatural
et chorologique. Nous avons comptabilisé 566 espèces relevant de 76 familles. Les résultats montrent une diversité
importante de la flore régionale qui est essentiellement dominée par les Asteraceae, les Fabaceae et les Poaceae. Les
thérophytes dominent le spectre biologique de cette flore qui se présente globalement comme une flore méditerranéenne
typique. L’analyse des types chorologiques montre la prédominance de l’élément méditerranéen avec un pourcentage
d’endémisme assez intéressant.
Mots clés : catalogue, diversité floristique, flore, forêts, inventaire, Tiaret

La wilaya de Sétif, réputée par sa richesse floristique et pour sa diversité
écologique, héberge une biodiversité floristique remarquable. Cette biodiversité nécessite une
bonne investigation sur terrain afin de localiser, d’identifier et de cartographier les espèces rares
et menacées de cette région. Parmi ces espèces, les orchidées demeurent les plus emblématiques
en raison de leur beauté, mais aussi de la fragilité de leurs habitats et des menaces qui pèsent
sur leur préservation. Les auteurs présentent dans cet article les nouvelles stations d’orchidées
découvertes dans la wilaya de Sétif. Vingt-six (26) espèces et sous-espèces d’orchidées ont été
observées, identifiées et géolocalisées. Parmi ces espèces, Orchis purpurea subsp. purpurea,
Dactylorhiza maculata subsp. battandieri, Orchis mascula subsp. maghrebiana, Ophrys
omegaifera subsp. hayekii et Ophrys marmorata subsp. marmorata ont été observées pour la
première fois dans la région. Dactylorhiza elata s.l. est bien représenté, mais seulement dans
des zones humides soumises à des dégradations humaines récentes ce qui fait craindre pour sa
pérennisation.
Mots clés. – Orchidaceae, Anacamptis, Cephalanthera, Dactylorhiza,
Himantoglossum, Ophrys, Orchis, Platanthera, Serapias, Djebel Babor, Djebel Bou Taleb,
Djebel Meg

Evaluation of in vitro antibacterial and antioxidant activities of essential oil and organic extracts
(methanolic, chloroformic and hexanic) of the aerial part of an aromatic and medicinal plant harvested in
southern Algeria, belonging to the Asteraceae (Rhanterium adpressum Cosson & Durieu).
The agar diffusion method was used to assess the antimicrobial activity of different extracts. These
extracts were tested against eleven (11) microbial strains, ten (10) of them are bacterial species (gram
positive and gram negative) and one (01) is yeast species. To evaluate the antioxidant activity, the DPPH
free radical catching method was used.
For the antimicrobial activity, all extracts showed inhibitory ability against at least one of the tested
microbial strains, the essential oil showed the best antibacterial and antifungal activity followed by the
hexanic (In-Hex), the chloroformic (EDCM) and the methanolic (EMeOH) extracts respectively. In fact,
nonpolar extracts are the most active. The results of the antioxidant activity showed that the methanolic
extract (EMeOH) presented the best antiradical capacity, followed by the chloroformic (EDCM) and the
hexanic (In-Hex) extracts and ultimately the essential oil. Indeed, methanol is the best solvent for the
concentration of the plant active substances. Extracts of Rhanterium adpressum have antioxidant and
antimicrobial activities.
Keywords: Antibacterial, antioxidant, Rhanterium adpressum, Algeria

This study is to assess the antimicrobial and antiradical activities of organic extracts from the species Pulicaria arabica (L.) Cass.
We used four clinical strains and four reference strains, to assess the antimicrobial activity of organic extracts of P. Arabica (L.)
Cass. Evaluation of the antiradical activity is done by spectroscopic assay using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method.
Measurement of the antiradical activity of three plant extracts was carried out using the DPPH test.
The anti-radical tests of ours extracts were assessed using the free radicals DPPH catching method. The results showed clearly, that
the methanol extract (EMeOH) had the best anti-radical ability followed by chloroform extract (EDCM) and lastly the hexane
extract (In-Hex). The antimicrobial tests were evaluated against reference pathogenic strains and others isolated from pathological
materials, using agar diffusion method. The results indicated that the apolar extracts (hexane and chloroform) had inhibitory activity
against a number of microorganisms higher than that of the polar methanolic extract.
Antimicrobial and antiradical activities of organic extracts from the species Pulicaria arabica (L.) Cass has shown that these
extracts have considerable inhibitory activity against certain germs and good potential of DPPH free radical scavenging, but these
activities are generally lower than those obtained with the reference products (antibiotics, antifungal and ascorbic acid).
Keywords: Pulicaria arabica, Organic extracts, Antimicrobial activity, Antiradical activity