Department
Departement of Nature and life sciences
Research Interests
On the Web:
-
Google Scholar N/A
-
ResearchGate N/A
-
ORCID N/A
-
SCScopus N/A
Recent Publications
IRIDOIDS AND FLAVONOIDS PRESENT IN DECOCTIONS FROM AERIAL PARTS OF VERBASCUM BETONICIFOLIUM
which comprises more than 300 species of wild growing plants, Verbascum betonicifolium (V. betonicifolium) is a plant used in traditional medicine for several ailments. The objective of this study was to determine the first phytochemical characterization of aqueous extracts from aerial parts. Methods: The compounds present in the aerial part aqueous extract were identified by high performance liquid chromatography, coupled to a diode-array detector (HPLC-DAD) and by high-resolution mass spectrometry (HRMS), using LC–MS/MS analyses. Results: Six types of plant metabolites were found: primary metabolites like malic, gluconic and citric acids, representing 36 % of the identified compounds; secondary metabolites like flavonoid derivatives (apigenin, luteolin) and several glycosylated derivatives, representing 27 % of the identified compounds; caffeic acid derivatives (chlorogenic acid) and several flavonoid derivatives representing 9 % of the identified compounds; iridoids like methylscutelloside, scropheanoside, saccatoside and so on, representing 30 % of the identified compounds; one lignan and a lactone sesquiterpenoid were also tentatively suggested.Conclusions: These results show for the first time the richness of phytochemicals of V. betonicifolium and that the aqueous extract could be used as new natural sources of bioactive molecules.
Citation
M. MERABTI Karim, (2023), "IRIDOIDS AND FLAVONOIDS PRESENT IN DECOCTIONS FROM AERIAL PARTS OF VERBASCUM BETONICIFOLIUM", [national] Séminaire national sur Les Substances Bioactives (SBIO-2023) 14 Mars 2023 , univ de ghardaia
Inventaire des lichens épiphytes des cédraies de la région de Hodna (M’Sila, Algérie)
An inventory of Hodna Cedar Epiphytic Lichen has been completed. Thirty stations spread over three study areas were studied and characterized by the calculation of various parameters. After the collection and identification of the samples, the work ends with the census of 51 lichenic taxa divided into 14 families, the most represented of which are the Physciaceae 16 taxa, Parmeliaceae 7, Lecanoraceae 6, Ramalinaceae 6 taxa.
From the physiological point of view, the foliated lichens are dominant with 44.31% followed by the crustaceans 34.09%, the fruticose 15.36 and finally the scaly and gelatinous and lepers with respectively 2.27%, 02.27 and 01.13. Specifically, 31 lichen species have been identified on the cedar, with a clear dominance of the genus Physcia and Parmeliafollowed by the genus Lecanoraand then Ramalina. From the point of view of lichen floristic richness the cedar grove represents a ratio of 31/51 species ie (60.78%) of the local lichen diversity.
Note that the Ouanoughacedraie is richer than the Maadidcedraie this can be partly explained by the fact that the latter is much more degraded (in advanced decline), than the first one that benefits a little from the influences of the Djurdjura.
Keywords: Inventory, Lichens, Cedars, Hodna Mountains
Citation
M. MERABTI Karim, (2022-11-02), "Inventaire des lichens épiphytes des cédraies de la région de Hodna (M’Sila, Algérie)", [national] EcoAgriTourism , Kellaf Rebbas
Les macro-lichens de la forêt d’Errich (Bouira, Algérie)
Citation
M. MERABTI Karim, Mohammed Ait hammou, , (2021), "Les macro-lichens de la forêt d’Errich (Bouira, Algérie)", [international] International Seminar on Biodiversity, Valorization and Conservation of Urban and Forest Ecosystems , Univ. M'sila
Inventaire des lichens des cédraies de la région de Hodna
Citation
M. MERABTI Karim, Mohammed Ait hammou, , (2021), "Inventaire des lichens des cédraies de la région de Hodna", [international] International Seminar on Biodiversity, Valorization and Conservation of Urban and Forest Ecosystems: (In support of sustainable development) , Univ. M'sila
Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium
activity of the aqueous extract together with the metabolites responsible for these activities. This paper presents
the first phytochemical characterization and bioactivities of aqueous extracts from aerial parts.
Methods: The compounds present in the aerial part aqueous extract were identified by high performance liquid
chromatography, coupled to a diode-array detector (HPLC-DAD) and by high-resolution mass spectrometry
(HRMS), using LC–MS/MS analyses. Antioxidant activity was measured as the capacity to scavenge the free
radical DPPH and the AChE activity was determined using the Ellman test. The cytotoxicity was determined used
HepG2 cell lines.
Results: Several types of metabolites were found, primary metabolites (malic, citric and gluconic acids), phenolic
compounds (verbascoside, luteolin), terpenoids (iridoid glycoside, unedide), among others. The total phenol
content of 28 μg of gallic acid equivalents/mg of extract was determined. The aqueous extract antioxidant
activity had and EC50 of 70 μg/mL and the AChE inhibitory activity an IC50 of 750 μg/mL. No cytotoxicity
towards HepG2 cells was detected, even using a concentration of 1 mg/mL.
Conclusions: The phenolic compounds present in the extract may be the main contributors to the bioactivities of
V. betonicifolium. These results show for the first time the richness of phytochemicals and the strong bioactivities
of V. betonicifolium and that the aqueous extract could be used as new natural sources of bioactive molecules.
Citation
M. MERABTI Karim, Fadel Sezan, Laura Guedes, Rebeca André, Rita Pacheco, Rita Guedes, Mohamed Djamel Miara, Maria Luísa Serralheiro, , (2020), "Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium", [national] European Journal of Integrative Medicine , elsevier