M. KHALFA Hanane

MCB

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Department

Departement of Nature and life sciences

Research Interests

Specialized in Departement of Nature and life sciences. Focused on academic and scientific development.

Contact Info

University of M'Sila, Algeria

Email : hanane.khalfa@univ-msila.dz

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Recent Publications

2024-12-16

Valorisation of Algerian medicinal plants : exploring polyphenol content and antioxidant activities.

Valorisation of Algerian medicinal plants : exploring polyphenol content and antioxidant activities.
Citation

M. KHALFA Hanane, Zaak Hadjer, Souilah Nabila, , (2024-12-16), "Valorisation of Algerian medicinal plants : exploring polyphenol content and antioxidant activities.", [national] Séminaire National sur la Valorisation des Bioressources dans les Régions Arides (VBRA- 2024), 16-17 Décembre 2024 , CRSTRA-Biskra. , CRSTRA-Biskra.

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2023-11-09

Phytochemical and biological potentialofPrickly pear (Opuntia ficus-indica) extracts from M’sila(Algeria)

ABSTRACTArticle history: Received 14February2023Revised 31Mai2023Accepted 09November2023The cladodes juice and seedsoil obtained from the prickly pear (Opuntia ficus-indca)grown in M’sila, Algeriaareinvestigated inthis work.The objective of the present study was to evaluate theirphysicochemical composition,polyphenols and flavonoid content, antioxidant potentialby DPPH testand antibacterial activities.The results of this experimental study showedthat leaf bud juice presented thehighest yield of79.02%.Leaf juice was rich in polyphenols (4.6 g/100 g) and flavonoids (38.35 mg/100 g). While, the study revealed that the seedsoil extracts presented a weak yield of 5%.Leafy sap from spinyspecies showshigh antioxidant activityand an antibacterialeffect on: S.aurous, E.coli, S.typhimirum, S.marcesscens. Whereas,the antibacterial activities were as follows:for leafy sap from spinyspecies E. coli, P. aeruginosa,S. typhimirum andK. pneumoniaestrains wererelatively resistant to oil,E.coliand S. marcescensstrainswere relativelyresistant to cold-pressed oil. The threeoil varietiescold,90°, and 160°pressed oil, were the most proven oils tobe effective against E. coli, P. aeruginosa, S.typhimirum and K. pneumonia.
Citation

M. KHALFA Hanane, (2023-11-09), "Phytochemical and biological potentialofPrickly pear (Opuntia ficus-indica) extracts from M’sila(Algeria)", [national] Alger.j. biosciences04 , univ eloued algeria

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2023

Phytochemical Profiling, LC-MS Analyses, Cytotoxicity, Antioxidant, and Antimicrobial Activities of Ruta Montana: An Ethnomedicinally Important Plant in Algeria Address

Objective: The present work aims to study the phytochemical composition and to evaluate the biological activities of R. montana L. (Rutacea) extracts.
Methods: Bioactive compounds were obtained using solid-liquid extraction using solvents of increasing polarity. The obtained extracts were qualitatively analyzed by liquid chromatography coupled with mass spectrometry (LC-MS). The pharmacological properties of R. montana were also investigated. Antioxidant activity was achieved "in vitro" using two methods: scavenging of the free radical DPPH and total antioxidant capacity. Antimicrobial activity was evaluated using disc diffusion method on 3 pathogenic bacterial strains (Escherichia Coli, Pseudomonas aeruginosa and Staphylococcus aureus). In addition, cytotoxic activity was determined by the "Brine shrimp" test.
Results: Chemical investigation allowed the identification of 14 phenolic compounds. The identified compounds were mainly phenolic acids, coumarins and flavonoids. The crude extract and the different tested fractions exhibited an interesting antioxidant activity. Chloroform extract was effective against the growth of the tested bacterial strains with zones of inhibition varying between 14 and 18 mm. In contrast, ethyl acetate and butanolic extracts were almost inactive on all of the tested bacterial strains. Furthermore, the crude extract was found to exhibit antibacterial activity with 11 to 11.66 mm of inhibitions zone. No cytotoxic effect was recorded for all the tested extracts up to a concentration of 4000 μg/mL.
Conclusion: This work highlights the potent bioactivity and acceptable drug-likeness of this plant, which supports its further uses.
Citation

M. KHALFA Hanane, Mustapha Mounir Bouhenna, Narimane Segueni, Asma Djadoudi, Soumeya Tahraoui, Abderrahim Benslama, Aicha Ksouri, Abdenour Boumechhour, Mohamed Djamel Miara, , (2023), "Phytochemical Profiling, LC-MS Analyses, Cytotoxicity, Antioxidant, and Antimicrobial Activities of Ruta Montana: An Ethnomedicinally Important Plant in Algeria Address", [national] Current Bioactive Compounds , Current Bioactive Compounds

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IRIDOIDS AND FLAVONOIDS PRESENT IN DECOCTIONS FROM AERIAL PARTS OF VERBASCUM BETONICIFOLIUM

Introduction: Verbascum L. is the largest genus of the Scrophulariaceae family,
which comprises more than 300 species of wild growing plants, Verbascum betonicifolium (V. betonicifolium) is a plant used in traditional medicine for several ailments. The objective of this study was to determine the first phytochemical characterization of aqueous extracts from aerial parts. Methods: The compounds present in the aerial part aqueous extract were identified by high performance liquid chromatography, coupled to a diode-array detector (HPLC-DAD) and by high-resolution mass spectrometry (HRMS), using LC–MS/MS analyses. Results: Six types of plant metabolites were found: primary metabolites like malic, gluconic and citric acids, representing 36 % of the identified compounds; secondary metabolites like flavonoid derivatives (apigenin, luteolin) and several glycosylated derivatives, representing 27 % of the identified compounds; caffeic acid derivatives (chlorogenic acid) and several flavonoid derivatives representing 9 % of the identified compounds; iridoids like methylscutelloside, scropheanoside, saccatoside and so on, representing 30 % of the identified compounds; one lignan and a lactone sesquiterpenoid were also tentatively suggested.Conclusions: These results show for the first time the richness of phytochemicals of V. betonicifolium and that the aqueous extract could be used as new natural sources of bioactive molecules.
Citation

M. KHALFA Hanane, (2023), "IRIDOIDS AND FLAVONOIDS PRESENT IN DECOCTIONS FROM AERIAL PARTS OF VERBASCUM BETONICIFOLIUM", [national] Séminaire national sur Les Substances Bioactives (SBIO-2023) 14 Mars 2023 , univ de ghardaia

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2022

Phytochemical characterization of opuntia Ficus-indica extracts

Prickly pear cactus is a dry climate-adapted plant that remains unexplored in Algeria. Prickly pear cladodes and seeds are rich in bioactive components that are beneficial to health. The juice obtained from cladodes and seed oil were the subject of this study. Physicochemical analysis, Total Phenolic Content (TPC), flavonoid content, antioxidant by DPPH test and antibacterial activity were our objectives. The yield of leaf bud juice extraction was 79.02%, while, the seed oil extraction yield was weak (5%). Physicochemical analysis and TPC showed that leaf juice was rich in polyphenols (4.6 g/100 g) and flavonoids (38.35 mg/100 g), leafy sap from spiny species gave high marks for antioxidant activity, and has got an effect on some bacteria: Staphylococtus aurous, E. coli, Salmonella typhimirum, Serratiamarcesscens. Cladode juice was rich in nutriments and has a potential antioxidant activity. The antibacterial activities shows that E. coli, Pseudomonas, Salmonella, and Klebsiella pneumoniae strains were relatively resistant to oil, and E. coli and S. marcescens strains were relatively resistant to cold-pressed oil. The 3 oils, cold pressed oil, 90° pressed oil and 160° pressed oil, were effective against E. coli, Pseudomonas, Salmonella and Klebsiella pneumoniae (11 to 19 mm of inhibition zone).
Citation

M. KHALFA Hanane, Benyahia Rahil Soundes, DEGHICHE Bochra, , (2022), "Phytochemical characterization of opuntia Ficus-indica extracts", [national] Premier séminaire national sur les substances bioactives , Université Mohamed Boudiaf-M’sila.

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Diversity and Traditional Use Value of Medicinal Plants in Bou Saada District of M’Sila Province, South East Algeria

The study aimed at identifying the plants of Bousaada district of M'sila province, South East Algeria, used by the local people as traditional medicines and analyzing their use value. The ethnomedicinal use information was collected by interviewing local informants using structured questionnaires through regular field surveys. A total of 193 species belonging to 69 families was identified. Lamiaceae and Asteraceae were the most commonly reported medicinal plants with 85 and 71 species, respectively. Artemisia herba-alba and Juniperus oxycedrus were the most widely used plants as the traditional medicine by the local population. The highest use value (UV) was observed for Citrus lemon (L.) Burm., Ficus carica L., Moringa oleifera Lam. and Olea europaea L. (UV=5). The highest fidelity level (FL) value was for 73 species. The calculated informant consensus factor (ICF) showed that diseases related to gastrointestinal disorders and diseases of the glands attached to the digestive system diseases present the highest values.
Citation

M. KHALFA Hanane, Miara MD, , (2022), "Diversity and Traditional Use Value of Medicinal Plants in Bou Saada District of M’Sila Province, South East Algeria", [national] Journal of Biodiversity Conservation and Bioresource Management , Official journal of the Biodiversity Conservation Trust Foundation (BCTF)

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USES PROFILE OF MEDICINAL PLANTS BY THE PEOPLE IN THE RURAL AREA OF BOUSAADA, (MSILA PROVINCE, SOUTH EST OF ALGERIA

This study aims to know the different uses of medicinal plants collected for
medicinal purposes by the local people of Bousaada (Msila province, Algeria). Semistructured interview questionnaires were used to collect and provide significant
ethnobotanical information on the plants used. The most widely used plants by the local
population in the traditional medicine are mainly Artemisia herba-alba, and Juniperus
oxycedrus. A total of 534 person were interviewed, most are women over 60 years old, with
a middle level of education, the most frequently used parts of plants for the treatment, was
leaves (33%) followed by seeds and fruits, the infusion (23%), followed by decoction
(20%), were represent to be used more, while, the most treated disease was gastrointestinal
disorders with a rate of 31.2%. Our study provided an opportunity to access and know
about the traditional uses of the inhabitants of Boussaâda, and showed that in the
Bousaada the folk use of plants still derives from daily practice. So, evaluation of
pharmacological activity for the important medicinal plants is suggested..
Citation

M. KHALFA Hanane, A. BOUFISSIOU, , (2022), "USES PROFILE OF MEDICINAL PLANTS BY THE PEOPLE IN THE RURAL AREA OF BOUSAADA, (MSILA PROVINCE, SOUTH EST OF ALGERIA", [national] Journal of EcoAgriTourism , Transilvania University Press

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