M. HARRAR Abdenassar

MCB

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Department

Departement of Microbiology and biochemistry

Research Interests

Specialized in Departement of Microbiology and biochemistry. Focused on academic and scientific development.

Contact Info

University of M'Sila, Algeria

Email : abdenassar.harrar@univ-msila.dz

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Recent Publications

2021

Séminaire National ‘webinaire’, Bio-ressources : Nutrition, Santé et Environnement - 17 et 18 Mai 2021 "BR-NSE21"

Comité d’organisation
Citation

M. HARRAR Abdenassar, (2021), "Séminaire National ‘webinaire’, Bio-ressources : Nutrition, Santé et Environnement - 17 et 18 Mai 2021 "BR-NSE21"", [national] Nutrition, Santé et Environnement , University Mohamed Boudiaf - M'sila

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2017

Phenolic Compounds, Antioxidant and Antibacterial Activities of Zizyphus lotus L. Leaves Extracts

Background: The use of plants for the treatment, cure and prevention of diseases is one of the oldest medicinal practices of humanity. These plants are able to produce much diversified natural compounds (e.g. alkaloids, polyphenols, etc.) which have many pharmaceutical applications.
Objective: The aim of the present study was to evaluate the antioxidant capacity and antibacterial activity of Zizyphus lotus extracts, which has been used traditionally in folk medicine of Algeria.
Methods: The leaves of plants were submitted to extraction by water and methanol to give methanolic (Met.E) and aqueous (Aq.E). The extracts were analyzed by HPLC-TOF/MS and their antioxidant activity was determined using DPPH and ABTS free radical-scavenging test, when the antibacterial activity was determined using disc diffusion method.
Results: The quantitative analysis of polyphenols and flavonoids content showed that the Met.E had the highest amount (562.04 µg EAG/mg E and 66.34 µg EQ/mg E, respectively). The HPLC-TOF/MS analysis of Met.E reveals the presence of two major compounds, rutin and gentisic acid, when Aq.E contain rutin and quercetin-3-β-D-glucoside as a major compound. Results showed that the Met.E exhibited the highest antiradical activity against DPPH and ABTS free radical with EC50=28.19±0.51 µg/mL, and EC50=3.82±0.03 µg/mL, respectively. Moreover, the Met. E of Z. lotus presents a moderate antibacterial activity against Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis, with inhibition zone ranging from 09 to 13 mm. In addition, The Met.E showed a better MIC against M. luteus and B. subtilis (MIC= 25 µg/mL).
Conclusion: Our results showed that the methanolic extract of Z. lotus reported a considerable antioxidant and antibacterial activities which may be due to their richness on polyphenols and flavonoids.
Keywords: Antioxidant, antibacterial, Z. lotus, phenolic compounds, HPLC-TOF/MS, disc diffusion method, methanolic (Met.E) extract.
Citation

M. HARRAR Abdenassar, Fatih GÜL, Ibrahim DEMİRTAŞ, , (2017), "Phenolic Compounds, Antioxidant and Antibacterial Activities of Zizyphus lotus L. Leaves Extracts", [national] The Natural Products Journal , Bentham science

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2016

Total phenolic contents, antioxidant and antibacterial activities of Limoniastrum guyonianum (Plombaginaceae) extracts

This work was carried out to study the antioxidant capacity and antibacterial activity of Limoniastrum guyonianum (Plombaginaceae) extracts, traditionally used in folk medicine in Algeria. The aerial part were submitted to extraction by water and methanol to give methanolic (Met.E) and aqueous (Aq.E) extracts with that yields are 10%and 17,5% respectively. Quantitative analysis of polyphenols and flavonoids content showed that the Met. E had the highest amount (285.37µg EAG/mgE and 44.11µg EQ/mg E, respectively). The antioxidant activity of extracts was evaluated by 2,2’-diphenyl-1-picrylhydrazyl (DPPH) and ABTS free radical-scavenging test. Results showed that the Met. E exhibited the highest antiradical activity against DPPH and ABTS free radical with EC50=0,099 mg/ml, and EC50=0.024mg/ml, respectively. Moreover, the antibacterial activity was determined on the five bacterial strains, using disk diffusion method. The Met. E showed inhibition zone averages only on M. luteus, and S. aureus (12 and 13 mm respectively) and with MIC is 25 μg/ml. Our results showed that the methanolic extract of L. guyonianum reported a considerable antioxidant and antibacterial activities which may be due to their richness on polyphenols and flavonoids.
Citation

M. HARRAR Abdenassar, Safia AFISSA, Asma SAIDI, , (2016), "Total phenolic contents, antioxidant and antibacterial activities of Limoniastrum guyonianum (Plombaginaceae) extracts", [national] International Journal of Current Research , akhil kapio

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