M. SARRI Madani

We report in this work the presence of an Asteraceae in M’Sila. Helianthus annuus species (the genus is very close to the genus Verbesina) is not reported in the flora of Algeria as weed, invasive or ruderal. The species ranked in the Tribe of Heliantheae which includes 4 genres including Verbesina, Ambrosia, Xanthium and Bidens in the flora of Quezel; Santa (1962-1963). Observed for a few years in the municipality of M’Sila at several different sites (roadside, rubble, cultivated land and foot of an ornamental tree).

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Le dépouillement des travaux de recherches (projet de recherche, thèse doctorat, mémoires d’ingénieur et de master) effectuer dans la région de M’sila sur la flore et la végétation depuis 2005 nous ont permis de récolter d’importantes informations écologique et de faire l’inventaire floristique de la région. Les résultats obtenus ont aboutis a 548 espèces appartenant à 65 familles et 317 genres dont 28 plantes endémiques, 77 plantes rares, 12 plantes protégés par la loi algérienne, 3 espèces sont classées dans la liste rouge de l’ICUN et dont 175 espèces propre à la steppe. La composition systématique de ces espèces fait apparaitre que 7 familles botaniques sont dominantes : les Asteraceae avec 73 espèces, les Fabaceae avec 56 espèces, les Poaceae avec 50 espèces, les Brassicaceae avec 47 espèces, les Lamiaceae avec 28 espèces, les Liliaceae avec 21 espèces et les Caryophyllaceae avec 20 espèces. Les spectres chorologie et biologique montrent que les espèces de souches méditerranéenne et les thérophytes sont les plus représentatives. L’étude a située plusieurs sites d’importances écologiques et économiques remarquable qui mérite d'être protégé et même classé en zone protégée (parc, réserve et corridor...) dans le cadre du développement durable. Il est a précisé que cette biodiversité est convoitée et non protégée contre les usages et les activités des habitants (coupe de bois, pâturage, récolte de plantes médicinales et utiles, etc.).

Keywords: inventory and floristic diversity, rare species, protected, chorology, M’Sila (Algeria)

Keywords: traditional uses, plants, ethnobotany, flu (+ covid-19), M’sila (Algeria)

Keywords: Sisymbrium irio, Brassicaceae, GC-MS, isopropyl isothiocyanate, Diseases, Algeria

Keywords: Ormenis africana, Asteraceae, Essential oil, GC-MS, α-Bisabolol, Santolina alcohol, Eucalyptol, Boutaleb Mountains

This work aims to test in vitro biological properties and phytochemical composition of the organic fraction of distillate of Salvia hispanica seeds. Twenty-five bioactive compounds were identified, constituting 90.40% of the total organic fraction. The chemical composition of organic fraction revealed that fatty acids were the main component at 78.93%, followed by steroids (6.95%) and tocophérol (1.87%). The organic fraction showed interesting antioxidant activity for DPPH, DMSO, ABTS, PHE and FRAP tests. Furthermore, the in vitro enzyme inhibitory activity against α-amylase exhibited the best activity (IC50 = 34.97 ± 0.68 μg/mL). The results indicated that organic fraction displayed good antimicrobial effect.

The whole plant Saccocalyx satureioides, an endemic medicinal plant in
Algeria, was evaluated for its polyphenolic contents, antioxidant and antimicrobial
activities. The polyphenolic contents of the plant methanolic extracts ranged from 170.47
to 285.56 (μg GAEs/mg extract) and from 25.39 to 82.68 (μg QEs/mg extract), respectively.
High antioxidant activity was registered: DPPH average IC50 = 14.68 μg/mL, and β-carotene
average IC50 = 21.08 μg/mL. The essential oil and methanolic extracts from the aerial parts
and roots were tested against bacteria and molds. Different methods, including disk
diffusion, well diffusion, and microdilution tests for bacteria, were used. The essential
oil (February and June harvest periods) value 1.25 μL/mL was the MIC and the MBC for
all bacterial strains, except E. coli, K. pneumonia and Ps. aeruginosa. The average fungal
inhibition of the methanol extracts ranged from 5.10 to 71.25%, except for F. oxysporum. The
essential oil effect was 6.67-83.53% and 17.01-90.57% by the food poisoned and fumigation
methods, respectively. The preliminary phytochemical evaluation demonstrated the high
polyphenolic contents and effective antioxidant power of the plant extracts. This may
have an essential role in the antimicrobial effectiveness and indicate that the plant
contains high-quality bioactive molecules in addition to the essential oil

Chemical residues in food pose health risks such as cancer and liver issues. This has driven the search for safer natural alternatives to synthetic fungicides and preservatives. The aim of this study was to characterize the chemical composition of the essential oils (EO), determine the polyphenolic contents, and evaluate the in vitro antioxidant and antifungal activities of methanol extracts (ME), essential oils (EO), and powders from Rosmarinus officinalis L. (rosemary) and Thymus ciliatus (Desf) Benth. (thyme) from the M’sila region, Algeria. The chemical composition of the EOs was determined by GC-MS. R. officinalis EO was composed of 31 components, mainly camphor (41.22%), camphene (18.14%), and α-pinene (17.49%); T. ciliatus EO was composed of 58 components, mainly, in percentage, α-pinene (22.18), myrcene (13.13), β-pinene (7.73), β-caryophyllene (10.21), and germacrene D (9.90). The total phenols and flavonoids were determined spectrophotometrically, and the rosemary ME was found to possess the highest polyphenolic content (127.1 ± 2.40 µg GAE/mg), while the thyme ME had the highest flavonoid content (48.01 ± 0.99 µg QE/mg). The antioxidant activity was assessed using three methods: rosemary ME was the most potent, followed by DPPH (IC50 = 13.43 ± 0.14 µg/mL), β-carotene/linoleic acid (IC50 = 39.01 ± 2.16 μg/mL), and reducing power (EC50 = 15.03 ± 1.43 µg/mL). Antifungal activity was assessed for 32 pathogenic and foodborne fungi. Four methods were applied to the solid medium. Incorporating the powdered plant into the culture medium (at 10%) reduced the fungal growth to greater than 50% in 21.88% and 6.25% of all fungal isolates, for R. officinalis and T. ciliatus, respectively. The ME, applied by the well diffusion method (0.1 g/mL), was less effective. Different concentrations of EO were tested. Incorporating the EO into the culture medium (1500 μL/L) inhibited 50% of the molds to levels of 50 and 75% for R. officinalis and T. ciliatus, respectively, with the complete inhibition of four fungi. Fumigated EO (15 μL) inhibited 65% of the molds to levels of 65 and 81.25% for R. officinalis and T. ciliatus, respectively, with the complete inhibition of five fungi. There was little to no sporulation in conjunction with the inhibition. Our results revealed some of the potential of the studied plants to fight foodborne molds and presented their promising characteristics as a source of alternatives to chemical pesticides and synthetic preservatives. Further studies are needed to find adequate application techniques in the food safety area.

This study was carried out with the objective to investigate the essential oil chemical composition, essential oil and extracts biological activities of Hertia cheirifolia. The volatile components of the aerial parts of H. cheirifolia, obtained by hydrodistillation, have been analysed by GC/MS. Fifty-six compounds were identified accounting for 98.41% of the total oil. However, all the oil was characterized by the predominance of three components, α-Pinene (33.07%), 1-[1-Methyl-1-(4-methyl-cyclohex-3-enyl)-ethyl]-1H-pyrrole (28.08%) and Germacrene D (19.59%). The free radical scavenging activity of the essential and the extracts was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) model system. The antifungal activity showed that the essential oil, the methanolic extract and the aqueous extract of H. cheirifolia significantly reduced the growth of Fusarium graminearum, F. oxysporum f. sp. albedinis and F. culmorum.

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Afin de connaître et de recenser les usages traditionnelles des plantes médicinales chez les habitants des enquêtes ethnobotanique se font pratiquement chaque année et depuis l’année 2005 dans la wilaya de M'sila. Ce travail a été réalisé aléatoirement auprès de 100 enquêtés de différentes catégories sociales durant l’année 2023, afin de recueillir autant d'informations sur les méthodes traditionnelles de traitement de la grippe. Les analyses des informations récoltées (questionnaires) ont comptés 14 plantes médicinales appartenant à 14 genres et à 8 familles botaniques. L’étude a montré que les feuilles (38%), les fruits (25%) et les fleurs (19%) sont les parties les plus largement utilisées sous forme d’infusion (22%), d’huile (19%) ou de jus (14%). Les causes principales de la grippe pour les enquêtés des quelques communes de M’sila étaient le climat (froids) et les infections. De plus, pour la question sur le traitement du covide-19 les habitants ont confirmés qu’ils ont utilisés les mêmes traitements de la grippe.

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Pour connaître les usages traditionnelles des plantes médicinales par les habitants, 18 communes (parmi les 47 communes existante) de la wilaya de M'sila ont étés touché par des enquêtes ethnobotaniques réalisé aléatoirement auprès de 109 enquêtés. Il a été utilisé un ensemble de questions sur un site web ou différentes catégories d’habitants ont répondus au questionnaire online. Plusieurs informations sur les méthodes traditionnelles de traitement des maladies d’estomac ont été recueillies et analysées. Les résultats obtenus ont comptés 25 plantes médicinales appartenant à 23 genres et à 11 familles qui traites plus de 6 maladies de l’estomac (Diarrhée, Nausée, Brûlures, Constipation, Perte d’appétit et les Gaz). L’étude a montré que les feuilles (43,3%) et les graines (25%) sont las parties les plus largement utilisée sous forme d’ébullition (63,8%). L’enquête a recensées 20 recettes composées exclusivement de plantes, elles sont soit constituées d’une seule plante soit associées avec d’autres plantes. Les universitaires sont les plus touchés par cette enquête vue la disponibilité et l’accès au net.

Keywords: Boraginaceae; medicinal plant; essential oils;GC–MS; Algeria

The whole plant Saccocalyx satureioides, an endemic medicinal plant in Algeria, was evaluated for its polyphenolic contents, antioxidant and antimicrobial activities. The polyphenolic contents of the plant methanolic extracts ranged from 170.47 to 285.56 (μg GAEs/mg extract) and from 25.39 to 82.68 (μg QEs/mg extract), respectively. High antioxidant activity was registered: DPPH average IC50 = 14.68 μg/mL, and β-carotene average IC50 = 21.08 μg/mL. The essential oil and methanolic extracts from the aerial parts and roots were tested against bacteria and molds. Different methods, including disk diffusion, well diffusion, and microdilution tests for bacteria, were used. The essential oil (February and June harvest periods) value 1.25 μL/mL was the MIC and the MBC for all bacterial strains, except E. coli, K. pneumonia and Ps. aeruginosa. The average fungal inhibition of the methanol extracts ranged from 5.10 to 71.25%, except for F. oxysporum. The essential oil effect was 6.67-83.53% and 17.01-90.57% by the food poisoned and fumigation methods, respectively. The preliminary phytochemical evaluation demonstrated the high polyphenolic contents and effective antioxidant power of the plant extracts. This may have an essential role in the antimicrobial effectiveness and indicate that the plant contains high-quality bioactive molecules in addition to the essential oil.

The essential oil from the aerial parts of Apium nodiflorum (L.) Lag. (Apiaceae), collected in Ksob River (Algeria) and obtained by hydrodistillation, was analysed by GC-MS. Sixty-seven components have been identified, representing more than 98.7% of the total oil. The essential oil was found to be rich in terpinolene (32.9 ± 4.6%),
myristicin (10.6 ± 2.3%), myrcene (6.2 ± 1.1%), limonene (6.0 ± 0.9%), γ-terpinene (5.9 ± 1.2%) and (Z)-caryophyllene (5.3 ± 1.0%).

The aim of this study was to analyse the essential oil of Lavandula dentata from Algeria and to test the antioxidant and antimicrobial properties of this plant. The essential oil (EO) (57 constituents) included mainly α-pinene, β-pinene, nopinone, linalool, cryptone, and limonene. The plant polyphenolic contents and the antioxidant activity were determined. The antimicrobial effect of the EO and the methanolic extract (ME) was assessed against referenced and clinical bacterial strains, and also foodborne fungal isolates. The EO minimal inhibitory concentration (MIC) values varied from 0.25 to 4 mg/mL and minimal bactericidal concentrations (MBCs) were less than 8 mg/mL except for S. aureus, clinical Klebsiella, S. epidermidis, and B. subtilis. The mould strains were significantly inhibited by the EO (87.50% to 88.33%). The MIC values were 3.60–15.62 mg/mL and 0.5–4 mg/mL for ME and EO, respectively. The minimal fungicidal concentration (MFC) values ranged from 31 to 125 mg/mL and from 2 to 8 mg/mL for ME and EO, respectively.

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This article aims to overview the traditional uses, chemical compositions and biological activities of essential oils and extracts of Pulicaria arabica which is considered a traditional medicinal plant in the treatment of various diseases.The P. arabica is used in the treatment of several diseases such as antidiarrheal, anti schistosomiasis, digestive disorders, ulcers, anti-tobacco and skin diseases. Several different biological properties have been reported such as antimicrobial, antioxidant, analgesic, antipyretic, anti-inflammatory, insecticidal, hepatoprotective and nephritic effects. The chemical investigation of the P. arabica (aerial parts, leaves, stems and flowers) showed some qualitative and quantitative differences in the chemical composition, for example the major compounds for essential oils (Thymol, γ-Cadinene, α-Cadinol and epi-α-Cadinol), flavonoids and Caryophyllene derivatives.

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The aim of this study is to identify the medicinal plants used as a traditional pharmacopoeia in the Hodna region. The technique used is based on ethnobotanical
surveys and is to collect information from the villagers. The study identified seventy-three (73) species. They are divided into sixty-seven (67) genera and thirty-nine (39) botanical families among which the most dominant are the Lamiaceae (12 species), Apiaceae (8 species) and the Asteraceae with 5 species. The aerial part occupies the first place among the plant organs used. The majority of remedies are prepared as a decoction with a percentage of 43%, followed by an infusion with 29%. We note that a large diversity of diseases treated by its identified plants. The ICF factors ranging from 0.79 to 0.89 for the eight uses categories retained for this study. The results obtained during this contribution constitute a database for subsequent studies aimed at experimentally evaluating the biological and chemical potential of these plants.

Keywords: Medicinal plants, Traditional pharmacopoeia, Diseases, Algeria.

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In this study, the methanolic extract (ME) and the essential oil (EO) of the medicinal plant Artemisia campestris L. were investigated for their antioxidant activity and their antifungal efficacy on the postharvest storage decays; Botrytis cinerea Pers. and Penicillium expansum Link. The total polyphenolic and flavonoid contents were determined.
The ME had higher total polyphenolic and flavonoid contents (400.64 μg GAE/mg and 43.13 ± 0.14 μg QE/mg, respectively) than EO (27.47 ± 0.44 μg GAE/mg and 14.04 ± 0.82 μg QE/mg, respectively). The ME presented higher radical scavenging power than the BHT and its IC50 values were 11.71, 40.96 and 23.32 μg/mL for the DPPH, β-carotene bleaching and reducing power respectively. In the antifungal activity, the EO had the stronger effect on both molds, particularly at concentrations > 15 μL, ≥ 800 μL/L and ≥ 15 μL by fumigation, incorporation and disc-diffusion methods respectively, resulting in higher than 80% inhibition of B. cinerea mycelial growth, and from 50 to > 80% inhibition on P. expansum mycelial growth. Methanolic extract showed nearby 50% inhibition on both fungi. The EO MIC was less than 2.5 μL/mL which was shown as MFC for both molds. The bio-autography test has shown separated compounds of the ME having an inhibitor effect on spore germination. These results offer an advantage of suggesting A. campestris could be used as a material for extraction of certain antifungal chemicals for preventing spoilage in food items.
Keywords: Antifungal activity, antioxidant activity, Artemisia campestris, Botrytis cinerea, Penicillium expansum

The analysed essential oil in this study was obtained by hydrodistillation from the aerial parts of Santolina rosmarinifolia L. (Asteraceae) collected from Hodna area of Algeria. This species is a medicinal herb traditionally used in Algeria. Its essential oil has been analyzed by combining GC-FID and GC-MS. The analysis led to the identification of eighty-two components, representing 91.84% of the whole composition of the sample. The main components were capillene (32.8%), 1,8-cineole (15.1%) and β-myrcene (14.0%).
Keywords: Santolina rosmarinifolia L., Asteraceae, Essential oil, GC-MS Analysis, Algeria

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Medicinal and aromatic plants represent an outstanding source of green active ingredients for a broad range of real-world applications. In the present study, we investigated the insecticidal potential of the essential oils obtained from three medicinal and aromatic plants of economic importance in Algeria, Artemisia campestris, Pulicaria arabica, and Saccocalyx satureioides. Gas chromatography coupled with mass spectrometry (GC-MS) was used to study the essential oil chemical compositions. The three essential oils were tested against a mosquito vectoring filariasis and arboviruses, i.e., Culex quinquefasciatus, a fly pest acting also as pathogens vector, Musca domestica, and an agricultural moth pest, i.e., Spodoptera littoralis, using WHO and topical application methods, respectively. The essential oil from A. campestris, containing β-pinene (15.2%), α-pinene (11.2%), myrcene (10.3%), germacrene D (9.0%) (Z)-β-ocimene (8.1%) and γ-curcumene (6.4%), showed remarkable toxicity against C. quinquefasciatus (LC50 of 45.8 mg L−1) and moderate effects (LD50 of 99.8 μg adult−1) against M. domestica. Those from P. arabica and S. satureioides, containing epi-α-cadinol (23.9%), δ-cadinene (21.1%), α-cadinol (19.8%) and germacrene D-4-ol (8.4%), and thymol (25.6%), α-terpineol (24.6%), borneol (17.4%) and p-cymene (11.4%), respectively, were more active on S. littoralis showing LD50 values of 68.9 and 61.2 μg larva−1, respectively. Based on our results, the essential oil from A. campestris may be further considered a candidate ingredient for developing botanical larvicides.

This work describes the chemical composition, polyphenolic content, antioxidant, antibacterial and antifungal activities of essential oil (EO) and methanolic extracts (MEs) of Lavandula stoechas L., a traditional medicinal plant widely distributed in the Algerian Tell. The essential oil from aerial parts was analyzed by GC–MS. Thirty-eight components were identified, of which fenchone (55.42%), camphor (25.18%) were the main compounds of the EO. The amounts of total phenolics of the EO and MEs ranged from 8.12±0.01 to 71.34±0.09µg AGE/mg respectively; flavonoids were from 6.45±0.003 to 14.53±0.02µg QE/mg. Antioxidant activity evaluated using β-carotene/linoleic acid, DPPH and reducing power assays. The IC50 values were registered and compared with those of BHT. The antimicrobial activity towards 04 bacterial strains and 2 fungal strains was assessed using disc diffusion, well diffusion and micro-well dilution assays. The EO (15µl/ml) appeared ineffective on E. coli and less effective on B. cereus to effective on S. aureus and has complete inhibitory effect on K. pneumoniae. The fungal isolates were moderately sensitive to the EO. The MEs have significant effect on all tested microorganisms only at high concentrations. showed significant capacity compared to methanol extracts. The HE tested Lavendula stoechas should be used as easily accessible source of natural antioxidants and as a possible food supplement or in pharmaceutical applications.

Keywords - Lavandula stoechas, essential oil, methanolic extract, antioxidant activity, antimicrobial activity.

The family Asteraceae (Compositae) includes about 200 genera and 2000 species, out of which, the genus Pulicaria is widely distributed in Asia, Europe, and Africa. In Algeria, there are 6 species of Pulicaria, four of which are found in the Sahara among them, Pulicaria arabica (L.) Cass also called Inula arabica is a fragrant herb which is traditionally used to treat swelling and painful boils. Several Studies directed by many researchers, in order to assess the biological properties of members of the genus Pulicaria, in this work we try to evaluate biological activity of some extract of Pulicaria arabica (L.) Cass.
The study of organic extracts from the species Pulicaria arabica (L.) Cass. has shown that these extracts have considerable antioxidant activity.

Key words: Pulicaria arabica, Organics extracts, M’sila

The aim of this work is to determine the medicinal plants traditionally used by the population of the forest massifs like the djebels: Tafat, Anini and Megriss (Tell Sétifien, Setif, Algeria), a floristic and ethnobotanical study was carried out in this region. Floristically 59 species belonging to 36 botanical families were identified. The systematic composition of these species shows that two families are dominant, the Asteraceae and the Lamiaceae with 07 species each. Similarly, an ethnobotanical survey based on 100 questionnaires was carried out on 40 people according to their age (25 < age <75), and their sex (man = 30, woman = 10) allowed to collect a certain number of information. The results of this study suggest that these medicinal plants have multiple and varied therapeutic uses.

KEY WORDS: Ethnobotany, Medicinal plants, Traditional uses, Questionnaire, Tell Sétifien

Abstract: This study is aimed to analyze the essential oil composition obtained by hydrodistillation from the aerial parts of Santolina rosmarinifolia L. (Asteraceae), collected from Hodna area of Algeria. This species is a medicinal herb traditionally used many diseases in Algeria, was analyzed by GC-MS. Eighty-two components were identified, representing 91.84% of the whole composition of the sample. The main components were capillene, 1.8-cineole and β-myrcene, representing more than 61% of the total oil.

Keywords: Santolina rosmarinifolia L., Asteraceae, essential oil, capillene, 1.8-cineole, β-myrcene

Cette recherche exploratoire, tente de dresser l'état des lieux des plantes médicinales utilisées dans le domaine de la pharmacologie traditionnelle de la région du Hodna. L’objectif global de cette étude est de recenser une liste des plantes selon leurs usages traditionnels et au même temps les encadrer, les protéger et assurer leur développement dans un contexte législatif et réglementaire de protection de la biodiversité locale et nationale. La méthodologie optée est basée sur les enquêtes ethnobotaniques et la cueillette des informations auprès des villageois. L’étude ethnobotanique a été réalisée au niveau de 13 localités du Hodna entre janvier et septembre 2017, qui nous a permis de recenser soixante-dix-sept (77) espèces ; on note qu’un nombre très important de pathologies traitées par ses plantes a été recensé, ce qui nous a incités à créer une base de données multivariable qui nous aide à reconnaitre le type de gestion adéquate durable de ce patrimoine floristique médicinale.

Mots-clés : Ethnobotanique - Biodiversité - Plantes médicinales - Gestion durable - Hodna.

The genus Pulicaria Gaertn., belongins to the tribe Inuleae of the family Asteraceae, consists of ca. 100 species which are widely distributed from Europe to North Africa and Asia, particularly around the Mediterranean (Williams, 2000). It is represented in the flora of Algeria (Quezel et Santa, 1963 and Ozenda, 1983) by 13 species (P. crispa, P. laciniata, P. dysenterica, P. odora, P. mauritanica, P. filaginoides, P. sicula, P. arabica, P. vulgaris, P. undulata, P. lothei, P. volskonskyana and P. inuloides). The aim of this work is to study the antioxidant activity of extracts (methanolic and ethanolic) from aerial parts of Pulicaria arabica (L.) Cass. Antioxidant activity of the extracts was carried out using DPPH (1,1-diphenyl-2 picrylhydrazyl) radical scavenging assay. The results indicated a Hight capacity.
Key words: Pulicaria arabica; Extracts; Antioxidant activity, DPPH; M’sila

Essential oils from the aerial parts of 15 samples of Algerian Rosmarinus officinalis L. were analysed by gas chromatography (GC) and Gas chromatography/mass spectroscopy. Thirty eight components have been characterized; among the monoterpene hydrocarbons α-pinene, camphene and limonene were individuated as the main components; camphor, 1,8-cineole and borneol were the principal oxygenated compounds, caryophyllene, α-bisabolol and partly humulene were the most represented sesquiterpenes. The scavenging activity of the oils was determined by the DPPH model system. The SC50 (Scavenging Concentration) values were in the range 120.4-326.1 μL/mL, representing a moderate antioxidant effectiveness. Essential oils were evaluated for their antimicrobial activity against Escherichia coli and Staphylococcus aureus; as well as against ten fungal strains belonging to Aspergillus, Alternaria, Candida, Fusarium, Penicillium and Saccharomyces genera. The results showed a moderate antimicrobial activity. The Algerian rosemary essential oils could be promising sources of biologically active compounds if they receive further studies on their biological properties.

Keywords: Rosmarinus officinalis, essential oil, antimicrobial, antioxidant, GC/MS.

Abstracts: The genus Pulicaria Gaertn., belongins to the tribe Inuleae of the family Asteraceae, consists of ca. 100 species which are widely distributed from Europe to North Africa and Asia, particularly around the Mediterranean (Williams, 2000). It is represented in the flora of Algeria (Quezel et Santa, 1963 and Ozenda, 1983) by 13 species (P. crispa, P. laciniata, P. dysenterica, P. odora, P. mauritanica, P. filaginoides, P. sicula, P. arabica, P. vulgaris, P. undulata, P. lothei, P. volskonskyana and P. inuloides). The aim of this work is to study the antioxidant activity of extracts (methanolic and ethanolic) from aerial parts of Pulicaria arabica (L.) Cass. Antioxidant activity of the extracts was carried out using DPPH (1,1-diphenyl-2 picrylhydrazyl) radical scavenging assay. The results indicated a Hight capacity.

Key words: Pulicaria arabica; Extracts; Antioxidant activity, DPPH; M’sila

Abstract: This work describes the chemical composition, polyphenolic content, antioxidant, antibacterial and antifungal activities of essential oil (EO) and methanolic extract (ME) of Lavandula dentata L., a traditional medicinal plant widely distributed in North-West of Algeria. The essential oil from aerial parts was analyzed by GC–MS. Fifty-seven components were identified, of which β-pinene (15.23%), limonene (9.24%), linalool (8.07%), nopinone (7.84%) cryptone (6.83%) and α-pinene (5.13%) were the main compounds of the EO. The amounts of total phenolics and flavonoids of the ME were respectively 308.06 mg EAG / g and 27.01 mg EQ / g. Antioxidant activity evaluated using β-carotene/linoleic acid assay resulted in IC50 values of 220.366 ± 4.085 mg/ml and 22.46±0.320 μl/ml for the ME and the EO respectively, and in DPPH free radical assay, the IC50 values were 2.58 mg/ml and 201.31±2.81 μl/ml for the ME and the EO respectively. The antimicrobial activity towards 18 bacterial strains and 5 fungi strains was assessed using disc diffusion, well diffusion and micro-well dilution assays. The EO showed greater antimicrobial activity than the ME with MICs ranging from 0.25 to 4mg/ml (0.25mg/ml for Candida albicans ATCC10231) and CMB values ≤ 8mg/ml except for S. aureus ATCC6538, S. aureus ATCC43300, S. epidermidis ATCC12228, B. subtilis ATCC9372, and clinical Klebsiella for which the CMB values were > 8mg/ml. For the molds, applied EO by two tests (well and disc diffusion assay) caused very significant inhibition on all the mold strains with inhibition percentage ranging from 56.94% to 87.50% in the well diffusion assay and from 77.17% to 88.33% in the disc diffusion assay.

Keywords: Lavandula dentata, essential oil, methanolic extract, antioxidant activity, antimicrobial activity.

Abstract: This study is devoted to an ethnobotanical, chemical and biological activity study of Juniperus genus. The purpose of this synthesis study and to create a database on the Juniperus genus mentioned in the Algerian flora to develop, exploit the use of these plants in the therapeutic and chemical field is why not a part of the pharmacopoeia traditional.

Key words: Biodiversity – Juniperus - Biological activities - Phytochemistry – Ethnobotany.

This exploratory research attempts to establish the inventory of medicinal plants used in the field of traditional pharmacology in the Hodna region. The overall objective of this study is to list a list of plants according to their traditional uses and at the same time to supervise, protect and ensure their development in a legislative and regulatory context of protection of local and national biodiversity. The methodology chosen is based on ethnobotanical surveys and the collection of information from villagers. The ethnobotanical study was conducted at 13 Hodna localities between January and September 2017, which allowed us to identify seventy-seven (77) species; We note that a very large number of pathologies treated by her plants were identified, which prompted us to create a multivariable database that helps us to recognize the type of sustainable management of this medicinal floristic heritage.

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Mots clés: Asteraceae , D’Anacyclus monanthos , espéces endémique , Algérie .

The chemical composition of the essential oil from the aerial parts of Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries (Asteraceae) growing in a semi-arid region of Algeria was investigated for the first time. The essential oil was obtained by hydrodistillation and fully characterized by gas chromatography-mass spectrometry (GC-MS). A total of 97 compounds were identified. The essential oil was found to be rich in trans-chrysanthenyl acetate (9.8 ± 2.0%), (E)-β-farnesene (7.4 ± 1.5%), germacrene D (6.9 ± 1.3%) and myristicin (4.8 ± 0.8%).

Keywords: Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries, Asteraceae, essential oil composition, GC-MS, Algeria

résumé absent

L’etude ethnobotanique a été réalisée au niveau de 13 localités du Hodna entre janvier et septembre 2017, s’est fixée comme objectif l’évaluation du potentiel de la région en plantes médicinales spécifiquement les arbres et arbustes .Au total ,33espéces (14 arbres et19 arbustes) cits sont utilisées en médicinales traditionnelles. L’enquête a ciblé plusieurs personnes de la population locale dont 128 fiches constituent une base d’informations sur le type d’usage préféré par cette dérniere. les résultats de cette étude ont monté que la partie aérienne constitue la partie la plus ytilisée. La majorité des rémedes est prparée sous forme de décoction suivie par l’infusion. En signale, une multiple divresité sur l’ensemble des maladies traitées. Les résultats obtenus constituent une source d’information très onéreuse pour la région du Hodna quipourrait étre une base de données dans les domaines de la phytochimie et la pharmacologie.
Mots cles : Ethnobotanique, plantes médicinales, Questionnaire, Hodna.

Context: The study area called gate to the desert, the oasis is the closest to the Algerian coastline luxuriant vegetation, lies at the Monts Ouled Nail of the Saharan Atlas. The latter is characterized by the presence of a fairly specific Algeria dunes, presents a sizeable floristic and ecological diversity.
Objective: Highlight traditional usage of plants despite environmental characteristics.
Materials and Methods: Our study consists in the elaboration of a survey concerning the traditional use in the Bou Saada region especially Citrullus colocynthis (L.) Schrad. species. Ethnobotanical surveys were conducted in the study area during 2011-2012. His research has been conducted in collaboration with healers, herbalists and ordinary users.
Results: This analysis of Citrullus colocynthis (L.) has revealed its use by 69 people of different ages. In addition, it has multiple uses such as: rheumatism (50%), diabetes (24%), inflammation of skin (8%), bacterial infections (7%), diseases of prostates (8%) and female infertility (4%).
Conclusion: This study shows that the colocynth is traditionally used by people of both sexes belonging to age groups and socioeconomic levels and different intellectuals. At the end of this work the observations on traditional use of Citrullus colocynthis (L.) Schrad. can be useful to guide a research work in the chemical and clinical side.

Key Words: Traditional use, Citrullus colocynthis (L.) Schrad, Questionnaires cards, Bou Saada (Algeria).

Context: M'sila city occupies a privileged position in the central part of northern Algeria. The climate of this area is continental, subject in part to the Saharan influences of which vegetation is steppic.
Aims: Highlight traditional usage of plants despite environmental characteristics.
Methods: An ethnobotanical survey in the city of M'sila was conducted during the period 2011-2012 in collaboration with traditional practitioners, herbalists and healers. A total of 85 adults were able to determine the species and answer questions about the traditional use of plants in artisanal processing, nutritional and medicinal domains.
Results: Medicinal plants recorded in the city of M'sila were 36 divided into 16 families and 31 genera. Lamiaceae family predominates (27.8%), followed by Asteraceae (13.9%). Leaves are the most frequently used (27.4%), the aerial parts (18.5%) and thus the seeds (16.3%). It appears that the population is highly dependent on these plants that allow them to treat different pathologies (digestive, stomach, diarrhea, constipation, vomiting) with a percentage (18.6%), carminative (5.7%) and antidiabetic (12.2%). In general, the remedies are administered orally. Indeed, therapeutic use forms are: the tisane or decoction (44.7%), infusion (27.1%) and powder (12.2%).
Conclusions: The ethnobotanical survey conducted among traditional healers, herbalists and healers in the M'sila city has created an inventory of 36 species and a database that collected all the information on local and traditional therapeutic applications as well as all the diseases treated.
Keywords: Ethnobotany; ethnopharmacological; therapeutic applications.

résumé

résumé absent

The volatile components of the aerial parts of Saccocalyx satureioides Coss et Durieu, obtained by
hydrodistillation, have been analysed by GC and GC-MS. Forty-one components have been fully identified and grouped
into three classes, namely monoterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes. The oxygenated
monoterpenes, with 76.9%, was the most represented class, ααααα -terpineol (32.7%), thymol (22.8%), borneol (11.6%) and
carvacrol (6.9%) being the main components. p-Cymene (5.0%), camphene (2.9%), γγγγγ -terpinene (2.8%), ααααα -pinene (1.8%)
and limonene (1.5%) were the more significant monoterpene hydrocarbons. The concentration of sesquiterpenes was lower
than 3%.

Essential oils extracted by hydrodistillation from the aerial parts of 23 samples of Algerian Origanum glandulosum Desf. were analysed by gas chromatography (GC) and GC–mass spectrometry (MS). Overall, 30 components have been fully characterized. However, all the oils were characterized by the predominance of four components, thymol (18.5–73.1%), carvacrol (7.6–72.6%), p-cymene (1.7–18.5%) and γ -terpinene (1.1–18.7%). Cluster analysis of the identified components allowed us to establish the presence of three main groups, characterized by carvacrol, thymol and comparable amounts of both compounds, respectively. The free radical scavenging activity of essential oils was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) model system. The SC50 (scavenging concentration) values were in the range 16.2–26.7 μg/ml, representing a good antioxidant effectiveness. The roles of thymol and carvacrol, the main components of all oils, were estimated by measuring their stoichiometric factors. The essential oils were also evaluated for their antimicrobial activity by the agar disc diffusion method and the determination of minimum inhibitory concentration (MIC) against six standard strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus hirae, Candida albicans, Candida tropicalis). All microbial strains employed (Gram-positive and Gram-negative bacteria and yeasts) showed a fairly similar degree of susceptibility to the essential oils under investigation, although no evident difference was observed in their sensitivity. Furthermore, a similar level of toxicity was observed for all oils examined, with MIC values of 31.25–125.00 μg/ml. Finally, the addition of the emulsifier Tween 80 to the oil or to the agar markedly decreases the antimicrobial activity of the essential oils against all microbial strains employed, thus suggesting that the antimicrobial activity of the essential oils is dependent on the physicochemical characteristics of their components and also on the microbial strains employed.

KEY WORDS: Origanum glandulosum Desf.; Lamiaceae; antimicrobial and antioxidant activities; essential oil; cluster analysis; thymol; carvacrol

The volatile components of the aerial parts of Saccocalyx satureioides Coss et Durieu, obtained by hydrodistillation, have been analysed by GC and GC-MS. Forty-one components have been fully identified and grouped into three classes, namely monoterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes. The oxygenated monoterpenes, with 76.9%, was the most represented class, α-terpineol (32.7%), thymol (22.8%), borneol (11.6%) and carvacrol (6.9%) being the main components. p-Cymene (5.0%), camphene (2.9%), γ -terpinene (2.8%), α-pinene (1.8%) and limonene (1.5%) were the more significant monoterpene hydrocarbons. The concentration of sesquiterpenes was lower than 3%.

KEY WORDS: Saccocalyx satureioides Coss et Durieu; Lamiaceae; essential oil; gas chromatography (GC); mass spectrometry (MS).

Sur la base d’enquête ethnobotanique nous avons entrepris de recenser les plantes médicinales et utiles du Parc National d’El-Kala. Ce dernier est constitué d’une mosaïque d’écosystème très diversifiée. La flore peut être exploitée selon un plan de gestion spatio-temporelle et quantitative. Les résultats des questionnaires réalisés auprès des habitants du parc nous ont permis de dresser deux tableaux: Le premier concerne les plantes utilisées en médecine traditionnelle et le deuxième les plantes utiles (qui présente une utilisation autre que la médecine). Nous avons confection un herbier (l’échantillon de la plante est scanné directement puis, il est mis en herbier). Cette base de données peut être utilisée pour la réalisation de la pharmacopée algérienne
MOTS CLES: Ethnobotanique, recensement, herbier, plan de gestion et pharmacopée algérienne.

ABSTRACT: The essential oils obtained from Origanum glandulosum Desf., collected in four different localities of the S`etif region (north-eastern Algeria), were analysed by GC–MS and assayed for their antioxidative effectiveness. The antioxidant capacity of the oils was measured by the modified thiobarbituric acid reactive species (TBARS) assay, using egg yolk as oxidizable substrate in presence of the radical inducer 2,20-azobis-(2-amidinopropane) dihydrochloride (ABAP). The activity was compared with those of ˛-tocopherol and 2,6-ditertbutyl-4-methyl phenol (BHT, butylated hydroxytoluene). The four oils were also endowed with a high degree of activity at the lowest concentration (100 ppm). This activity is to be ascribed to the high content of phenol components, viz. thymol and carvacrol, which strongly characterize the composition of these oils.

KEY WORDS: Origanum glandulosum Desf; Lamiaceae; essential oil; antioxidant; TBARS assay; thymol; carvacrol