M. KHADIDJA Dehimi

MCA

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Department

Departement of Microbiology and biochemistry

Research Interests

Biochemistry, Phytotherapy

Contact Info

University of M'Sila, Algeria

Email : khadidja.dehimi@univ-msila.dz

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Recent Publications

2023

Acute Toxicity Evaluation of Aqueous Extract from Hertia cheirifolia in Swiss Albino Mice

Hertia cheirifolia L. is a medicinal plant belonging to Asteraceae family. It is traditionally used to treat gastrointestinal ailments, infections, and spasms. The aim of this study is to evaluate the oral acute toxicity of aqueous extract from Hertia cheirifolia on female Albino wistar mice. Animals were divided into three groups, the first group received distilled water and was used as control; Mice of the second and third groups received the plant extract at doses of 2g/kg and 5g/kg body weight of animals, respectively. The mice were monitored for 14 days for their general behavior, adverse effects and mortality. The results showed that no death or toxic signs were observed, no changes were seen in food consumption, body weight and organ weight in the treated animals compared to the controls. However, an increase in AST and ALT levels was recorded in mice treated with the extract.
Citation

M. KHADIDJA Dehimi, (2023), "Acute Toxicity Evaluation of Aqueous Extract from Hertia cheirifolia in Swiss Albino Mice", [national] Agricultural Mechanization in Asia, Africa and Latin America , Farm Machinery Industrial Research Corp and Shin-Norinsha Co.

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Antioxidant and antibacterial activities of crude extracts and essential oil from Thymus algeriensis

Thymus algeriensis, locally known as «Zaitra», is a medicinal plant which belongs to the Lamiaceae family. This species is widely distributed in all Algerian regions and used as spice and also to treat respiratory affections. In the present study, phenols quantification, antioxidant and antibacterial activities of crud extracts and essential oil from aerial parts of T. algerensis were evaluated. Extraction yields of methanolic and aqueous extracts were estimated to 8.2 and 8.5 %, respectively, while the yield of essential oil was 18.85%. The highest polyphenols content was found in methanolic extract (236.18 μg gallic acid equivalent/mg of extract). Methanolic extract showed the best scavenging activity against the DPPH radical (IC50= 0.79 mg/ml). The study of antibacterial activity showed that only methanolic extract has an inhibition against S. aureus, with an inhibition zone of 11.33 mm at a concentration of 250 mg/ml. Among tested strains, E. coli and S. aureus were extremely sensitive to the essential oil of the plant, with inhibition zones of 29 and 25.5 mm, respectively.
Key words: Thymus Algeriensis, crud extracts, phenols, antioxidant, antibacterial, essential oil.
Citation

M. KHADIDJA Dehimi, (2023), "Antioxidant and antibacterial activities of crude extracts and essential oil from Thymus algeriensis", [national] National seminar on phytotherapy and pharmacognosy NSPP 2023 , Ferhat Abbas University Setif

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2022

Evaluation of Antioxidant Activity and Polyphenols Content of Melilotus indicus Extracts

Melilotus indicus is a medicinal plant known for its ethno-medicinal activities such as antibacterial, anticoagulant, antispasmodic, emollient, laxative and narcotic effects. The objective of this study is to evaluate the antioxidant activity of aqueous extract (AqE) and ethanolic extract (EE) from Melilotus indicus using two methods: the DPPH (2, 2'-diphenyl- 1- picrylhydrazy) test and β-carotene bleaching assay. Moreover, phenolic compounds contents were estimated in both extracts. Results showed that total polyphenols content in ethanolic extract was 120.067 μg GAE/mgE and 144.382 μg GAE/ mgE for aqueous fraction. Flavonoids contents in studied extracts were very close (26.375 μg QE/mg of ethanolic extract and 25.65 μg QE/mg of aqueous extract). Aqueous fraction was found to possess a higher scavenging capacity against DPPH radical (IC50 = 0.09 ± 0.002 mg/ml) than ethanolic extract (IC50 = 0.28 ± 0.037 mg /ml). The inhibitory activity of the tow extracts in the β-carotene/linoleic acid assay was estimated to 70.983 ± 4.22 % for aqueous extract and 66.169 ± 3.445 % for ethanolic fraction.
Citation

M. KHADIDJA Dehimi, (2022), "Evaluation of Antioxidant Activity and Polyphenols Content of Melilotus indicus Extracts", [international] the 5. International Conference on Life and Engineering Sciences , Antalya-TURKEY

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Antioxidant activity and acute toxicity study of Centaurium erythraea extracts

Centaurium erythraea is a medicinal plant belonging to the Gentianaceae family. It is used in traditional medicine to treat various diseases such as diabetes, fever, rhinitis, stomach ailments, urinary tract infections, dyspeptic complaints, loss of appetite, and hemorrhoids, and as diuretic. In fact this plant is known also as an antiapoplectic, anticoagulant, anticholagogue, antipneumonic, hematocathartic, and as a hypotensive agent. The aim of the present study is to evaluate the antioxidant activity of methanolic and aqueous extracts from this plant using the DPPH free radical test, the β-carotene /linoleic acid method and the reducing power assay. Acute toxicity of aqueous extract was also estimated in mice after oral administration as a single dose of 2000 and 5000 mg/kg body weight and then animals were observed for 14 days for any toxicity signs. Total phenolic content was evaluated in both extracts where aqueous and methanolic extracts contained 54,27 and 35,45 μg GAE/mg of extract, respectively. The content of flavonoids was estimated using Aluminium Chloride method and it was equal to 6,65 μg QE/mgE in methanolic fraction and 3,27 μg QE/mgE in aqueous extract. In the DPPH assay, both fractions showed a powerful antioxidant activity with very close IC50 values (0.20 mg/ml for aqueous extract and 0.28 mg/ml for methanolic fraction). In the β-carotene bleaching assay, methanolic extract showed the best inhibitory activity (86.78 %) in comparison with aqueous fraction (77.81 %). Similarly, in the FRAP assay methanolic fraction gave the best activity (IC50 = 0.35 mg/ml) compared to aqueous extract (IC50= 1.31 mg/ml). Results of acute toxicity study showed that a single administration of aqueous extract up to a dose of 5000 mg/kg bw did not cause any mortality or signs of toxicity in treated mice. There were no significant differences in body weight, food consumption and absolute organs weight between controls and treated animals. Biochemical analysis showed no differences in parameters examined. No histological changes were observed in organs of treated mice compared to the control group.
Citation

M. KHADIDJA Dehimi, (2022), "Antioxidant activity and acute toxicity study of Centaurium erythraea extracts", [national] Séminaire national sur la valorisation des bioressources végétales en Algérie , Université de Khenchela

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Evaluation of antioxidant activity and acute toxicity of extracts from leaves and flowers of Thymelaea hirsuta

Not available
Citation

M. KHADIDJA Dehimi, (2022), "Evaluation of antioxidant activity and acute toxicity of extracts from leaves and flowers of Thymelaea hirsuta", [national] La valorisation des ressources naturelles et de l'environnement , Université de Sétif

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Effect of Different Extracting Solvents on Phenolic Content and Antioxidant Activity of Extracts From Algerian Marrubium vulgare

Marrubium vulgare (Lamiaceae), known in Algeria as «Merriouet», is a medicinal herb
originated in Mediterranean area and central Asia, nowadays is common in all continents. It is
used in folk medicine to treat many illnesses but especially digestive disorders. In the present
study, phenolic profile and antioxidant activity of M. vulgare extracts prepared using solvents
from different polarities are evaluated. According to the obtained results, total phenols content
was proportional to the polarity of solvent used for the extraction; aqueous extract showed the
highest amount in polyphenols with a value of 60.84 μg GAE/mg E. Acetone extract was
found to be the most rich in flavonoids (100.12 μg QE/mg E), while methanol fraction
contained the highest tannins content (81.29 μg TAE/mg E). In the FRAP assay, the capacity
of extracts to reduce iron was proportional to their tannins contents, the best activity was
obtained by methanol fraction with A = 0.94 at a concentration of 2 mg/ml. In the β-carotene /
linoleic acid test, the highest percentage inhibition was obtained by less polar extracts (hexane
and acetone) with a value of 84% after 24 h of incubation. Furthermore, all fractions did not
have any effect on E. coli, P. aeruginosa and S. aureus at a concentration of 200 mg/ml.
Key words: Marrubium vulgare, Solvent polarity, polyphenols, antioxidant.
Citation

M. KHADIDJA Dehimi, (2022), "Effect of Different Extracting Solvents on Phenolic Content and Antioxidant Activity of Extracts From Algerian Marrubium vulgare", [international] the 5. International Conference on Life and Engineering Sciences , Antalya-TURKEY

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Phenolic Profile, Antioxidant and Antibacterial Activities of Extracts From Algerian Bryonia dioica.

Bryonia dioica is a medicinal plant belonging to the Cucurbitaceae family; it is common in
Algeria in the Tell regions and locally called « Berestom ». This plant is widely used in
traditional medicine for the treatment of cancers and inflammatory diseases. In this study, the
antioxidant activity of extracts prepared from the tubers of the plant is evaluated by different
in vitro methods and antibacterial activity by means Agar well diffusion method. The
extraction of active substances is carried out by four solvents with different polarities (hexane,
acetone, methanol and distilled water), using the technique of maceration. Among studied
extracts, acetone fraction was the most rich in polyphenols (45.72 μg GAE/mg extract), in
flavonoids (14.36 μg QE/mg E) and in tannins (71.41 μg TAE/mg E). In the FRAP assay, the
same extract (acetone) showed the best capacity to reduce iron with a maximal absorbance
value of A = 0.67 at a concentration of 6 mg/ml. The highest percentage inhibition in the β-
carotene / linoleic acid test was obtained by hexane extract (45.74 % after 24h of incubation).
Furthermore, acetone fraction was the only active extract against S. aureus with an inhibition
zone of 15 mm at a concentration of 200 mg/ml.
Key words: Bryonia dioica, polyphenols, ferric reducing power, β-carotene bleaching,
antibacterial activity.
Citation

M. KHADIDJA Dehimi, (2022), "Phenolic Profile, Antioxidant and Antibacterial Activities of Extracts From Algerian Bryonia dioica.", [international] the 5. International Conference on Life and Engineering Sciences , Antalya-TURKEY

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Biological Activities of Extracts and Essential Oil of Thapsia garganica Roots Collected From The Region of M’sila in North-East Algeria

Thapsia garganica (Apiaceae) is an endemic herbal medicine growing spontaneously in the
Mediterranean area, known in Algeria as «Derias or «Bounafaa». It is used in traditional
medicine as diuretic, emetic and purgative, but especially as an anti-inflammatory agent in the
treatment of rheumatism. In the present study, phenolic profile, antioxidant and antibacterial
activities of extracts (hexane, acetone, methanol and aqueous) and essential oil from T.
garganica are evaluated. Among studied extracts, acetone fraction was the most rich in
polyphenols (35.93 μg GAE/mg extract), in flavonoids (17.65 μg QE/mg E) and in tannins
(73.02 μg TAE/mg E). In the FRAP assay, the same extract (acetone) showed the best
capacity to reduce iron with a maximal absorbance value of A = 0.83 at a concentration of 6
mg/ml. Interestingly in the β-carotene / linoleic acid test, the highest percentage inhibition
was obtained by hexane extract (75.82 % after 24h of incubation). Antibacterial activity of the
studied plant was evaluated using Agar well and Agar disc diffusion methods. The highest
inhibition zones were obtained by the plant essential oil against S. aureus and E.coli.
Key words: Thapsia garganica, polyphenols, essential oil, antioxidant, antibacterial.
Citation

M. KHADIDJA Dehimi, (2022), "Biological Activities of Extracts and Essential Oil of Thapsia garganica Roots Collected From The Region of M’sila in North-East Algeria", [international] the 5. International Conference on Life and Engineering Sciences , Antalya-TURKEY

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Antioxidant and Antimicrobial Activities of Extracts and Essential Oil of Artemisia herba-alba From The Region of M’sila (North-East of Algeria).

Artemisia herba-alba is a medicinal plant belonging to the Asteraceae family, known as
desert wormwood and in Arabic as « Chih ». In Algeria, this species is very common in
steppes. In traditional medicine it is used as antispasmodic, hypoglycemic, antdiabetic and to
treat hypertension. Extracts and essential oil from this plant are known for their antimicrobial,
antioxidant, insecticidal, and antispasmodic activities. In the present study, antioxidant and
antimicrobial activities of extracts (hexane, acetone, methanol and aqueous fractions) and
essential oil from A. herba-alba are evaluated. Aqueous extract was the most rich in
polyphenols (85.2 μg GAE/mg E), acetone fraction the richest in flavonoids (83.89 μg QE/mg
E) and methanol extract contained the highest amount of tannins (226.83 μg TAE/mg E). In
the FRAP assay, acetone fraction showed the best capacity to reduce iron with a maximal
absorbance value of A = 1.90 at a concentration of 2 mg/ml. The highest percentage inhibition
in the β-carotene / linoleic acid test was obtained by methanolic extract (87.54 % after 24h of
incubation). In antimicrobial activity assays, the plant extracts showed a good potency against
S. aureus and E.coli. Besides these two bacterial strains, essential oil from the studied species
exhibited a high activity against P. aeruginosa.
Key words: Artemisia herba-alba, polyphenols, essential oil, antioxidant, antibacterial.
Citation

M. KHADIDJA Dehimi, (2022), "Antioxidant and Antimicrobial Activities of Extracts and Essential Oil of Artemisia herba-alba From The Region of M’sila (North-East of Algeria).", [international] 5. International Conference on Life and Engineering Sciences , Antalya-TURKEY

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Chemical Constituents of the Essential Oil from Salvia Verbenaca ssp. Clandestina from Algerian Pre-Sahara

The essential oil obtained by hydrodistillation from the aerial parts of Salvia verbenaca (L.) Briq. ssp. clandestina (L.) Pugsl. ( Lamiaceae ) growing wild in Bou Saâda, pre-Saharan region of Algeria, was analyzed by GC-MS. Sixty-four compounds were detected, representing 95.6% of the whole oil, among them forty five compounds are identified in this sample for the first time. The essential oil of S. verbenaca ssp. clandestena showed the predominance of sesquiterpenes (56.4%) followed by monoterpene derivatives (35.5%). The main constituents were β-pinene (10.2%), spathulenol (8.7%), caryophylene oxide (6.1%), α-pinene (5.2%), germacrene D (5%) and α-gurjunene (4.9%). Chemical composition of the essential oil from our sample may be categorized as sesquiterpene and monoterpene chemotype among the four chemotypes identified for Salvia species.
Citation

M. KHADIDJA Dehimi, (2022), "Chemical Constituents of the Essential Oil from Salvia Verbenaca ssp. Clandestina from Algerian Pre-Sahara", [national] Acta Biologica Marisensis , Sciendo

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2021

ACUTE TOXICITY STUDY OF Sinapis alba AQUEOUS EXTRACT IN SWISS ALBINO MICE

Sinapis alba or white mustard is a medicinal plant that belongs to the brassicaceae family. It is traditionally used to draw blood away from underlying infections and to act as a counterirritant. The aim of this study is to evaluate the oral acute toxicity of aqueous extract from Sinapis alba on male Swiss Albino mice. Animals were divided into three groups, each group containing three mice. The first group received normal saline and was used as control; Mice of the second and third groups received the plant extract at doses of 2 g/kg and 5 g/kg body weight of animals, respectively. The mice were monitored for 14 days for their general behavior, adverse effects and mortality. The results showed that no death was recorded among animals; Furthermore, the extract did not cause any changes in body and organs weights. Although S. alba extract was found to be safe on kidneys, it caused an increase in alanine aminotransferase levels.
Citation

M. KHADIDJA Dehimi, (2021), "ACUTE TOXICITY STUDY OF Sinapis alba AQUEOUS EXTRACT IN SWISS ALBINO MICE", [national] Plant Cell Biotechnology and Molecular Biology , I. K. Press

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