M. SELLOUM Mounir

MAA

Directory of teachers

Department

Departement of Microbiology and biochemistry

Research Interests

Specialized in Departement of Microbiology and biochemistry. Focused on academic and scientific development.

Contact Info

University of M'Sila, Algeria

Email : mounir.selloum@univ-msila.dz

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Recent Publications

2023-09-01

Phytochemical analysis, antibacterial and antifungal effect of Lavandula dentata L. essential oil and methanol extract

The aim of this study was to analyse the essential oil of Lavandula dentata from Algeria and to test the antioxidant and antimicrobial properties of this plant. The essential oil (EO) (57 constituents) included mainly α-pinene, β-pinene, nopinone, linalool, cryptone, and limonene. The plant polyphenolic contents and the antioxidant activity were determined. The antimicrobial effect of the EO and the methanolic extract (ME) was assessed against referenced and clinical bacterial strains, and also foodborne fungal isolates. The EO minimal inhibitory concentration (MIC) values varied from 0.25 to 4 mg/mL and minimal bactericidal concentrations (MBCs) were less than 8 mg/mL except for S. aureus, clinical Klebsiella, S. epidermidis, and B. subtilis. The mould strains were significantly inhibited by the EO (87.50% to 88.33%). The MIC values were 3.60–15.62 mg/mL and 0.5–4 mg/mL for ME and EO, respectively. The minimal fungicidal concentration (MFC) values ranged from 31 to 125 mg/mL and from 2 to 8 mg/mL for ME and EO, respectively.
Citation

M. SELLOUM Mounir, Soumia Seghiour, Edoardo Napoli, Giuseppe Ruberto, , (2023-09-01), "Phytochemical analysis, antibacterial and antifungal effect of Lavandula dentata L. essential oil and methanol extract", [national] Natural Product Research , Taylor & Francis

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2021

Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L.

In this study, the methanolic extract (ME) and the essential oil (EO) of the medicinal plant Artemisia campestris L. were investigated for their antioxidant activity and their antifungal efficacy on the postharvest storage decays; Botrytis cinerea Pers. and Penicillium expansum Link. The total polyphenolic and flavonoid contents were determined.
The ME had higher total polyphenolic and flavonoid contents (400.64 μg GAE/mg and 43.13 ± 0.14 μg QE/mg, respectively) than EO (27.47 ± 0.44 μg GAE/mg and 14.04 ± 0.82 μg QE/mg, respectively). The ME presented higher radical scavenging power than the BHT and its IC50 values were 11.71, 40.96 and 23.32 μg/mL for the DPPH, β-carotene bleaching and reducing power respectively. In the antifungal activity, the EO had the stronger effect on both molds, particularly at concentrations > 15 μL, ≥ 800 μL/L and ≥ 15 μL by fumigation, incorporation and disc-diffusion methods respectively, resulting in higher than 80% inhibition of B. cinerea mycelial growth, and from 50 to > 80% inhibition on P. expansum mycelial growth. Methanolic extract showed nearby 50% inhibition on both fungi. The EO MIC was less than 2.5 μL/mL which was shown as MFC for both molds. The bio-autography test has shown separated compounds of the ME having an inhibitor effect on spore germination. These results offer an advantage of suggesting A. campestris could be used as a material for extraction of certain antifungal chemicals for preventing spoilage in food items.
Keywords: Antifungal activity, antioxidant activity, Artemisia campestris, Botrytis cinerea, Penicillium expansum
Citation

M. SELLOUM Mounir, faiza.boussakra, Ouahiba.deriche, , (2021), "Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L.", [national] International Journal of Agriculture, Environment and Food Sciences , http://dergipark.gov.tr/jaefs

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2020

Chemical composition, polyphenolic content, antioxidant and antimicrobial activities of essential oil and methanolic extract of Lavandula stoechas L.

This work describes the chemical composition, polyphenolic content, antioxidant, antibacterial and antifungal activities of essential oil (EO) and methanolic extracts (MEs) of Lavandula stoechas L., a traditional medicinal plant widely distributed in the Algerian Tell. The essential oil from aerial parts was analyzed by GC–MS. Thirty-eight components were identified, of which fenchone (55.42%), camphor (25.18%) were the main compounds of the EO. The amounts of total phenolics of the EO and MEs ranged from 8.12±0.01 to 71.34±0.09µg AGE/mg respectively; flavonoids were from 6.45±0.003 to 14.53±0.02µg QE/mg. Antioxidant activity evaluated using β-carotene/linoleic acid, DPPH and reducing power assays. The IC50 values were registered and compared with those of BHT. The antimicrobial activity towards 04 bacterial strains and 2 fungal strains was assessed using disc diffusion, well diffusion and micro-well dilution assays. The EO (15µl/ml) appeared ineffective on E. coli and less effective on B. cereus to effective on S. aureus and has complete inhibitory effect on K. pneumoniae. The fungal isolates were moderately sensitive to the EO. The MEs have significant effect on all tested microorganisms only at high concentrations. showed significant capacity compared to methanol extracts. The HE tested Lavendula stoechas should be used as easily accessible source of natural antioxidants and as a possible food supplement or in pharmaceutical applications.

Keywords - Lavandula stoechas, essential oil, methanolic extract, antioxidant activity, antimicrobial activity.
Citation

M. SELLOUM Mounir, Othmani Zineb, Edoardo Napoli, Giuseppe Ruberto, , (2020), "Chemical composition, polyphenolic content, antioxidant and antimicrobial activities of essential oil and methanolic extract of Lavandula stoechas L.", [national] 1ère Journée nationale sur Analyse de cycle de vie, Eco-Conception, Eco-éducation, Eco -innovation au service du développement durable , Algeria (Boumerdès)

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2019

Chemical composition, phenololic content, antioxidant and antimicrobial activities of essential oil and methanolic extract of Lavandula dentata L.

Abstract: This work describes the chemical composition, polyphenolic content, antioxidant, antibacterial and antifungal activities of essential oil (EO) and methanolic extract (ME) of Lavandula dentata L., a traditional medicinal plant widely distributed in North-West of Algeria. The essential oil from aerial parts was analyzed by GC–MS. Fifty-seven components were identified, of which β-pinene (15.23%), limonene (9.24%), linalool (8.07%), nopinone (7.84%) cryptone (6.83%) and α-pinene (5.13%) were the main compounds of the EO. The amounts of total phenolics and flavonoids of the ME were respectively 308.06 mg EAG / g and 27.01 mg EQ / g. Antioxidant activity evaluated using β-carotene/linoleic acid assay resulted in IC50 values of 220.366 ± 4.085 mg/ml and 22.46±0.320 μl/ml for the ME and the EO respectively, and in DPPH free radical assay, the IC50 values were 2.58 mg/ml and 201.31±2.81 μl/ml for the ME and the EO respectively. The antimicrobial activity towards 18 bacterial strains and 5 fungi strains was assessed using disc diffusion, well diffusion and micro-well dilution assays. The EO showed greater antimicrobial activity than the ME with MICs ranging from 0.25 to 4mg/ml (0.25mg/ml for Candida albicans ATCC10231) and CMB values ≤ 8mg/ml except for S. aureus ATCC6538, S. aureus ATCC43300, S. epidermidis ATCC12228, B. subtilis ATCC9372, and clinical Klebsiella for which the CMB values were > 8mg/ml. For the molds, applied EO by two tests (well and disc diffusion assay) caused very significant inhibition on all the mold strains with inhibition percentage ranging from 56.94% to 87.50% in the well diffusion assay and from 77.17% to 88.33% in the disc diffusion assay.

Keywords: Lavandula dentata, essential oil, methanolic extract, antioxidant activity, antimicrobial activity.
Citation

M. SELLOUM Mounir, SEGHIOUR Soumia, EDOUARDO Napoli, GIUSEPPE Ruberto, , (2019), "Chemical composition, phenololic content, antioxidant and antimicrobial activities of essential oil and methanolic extract of Lavandula dentata L.", [national] Premier Séminaire National: L'Apport des Biotechnologies sur la Protection de l'Environnement , Algeria (M'sila)

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2017

Inventaire et enquête ethnobotanique des plantes aquatiques d'Oued M'Sila (Ksob).

résumé absent
Citation

M. SELLOUM Mounir, (2017), "Inventaire et enquête ethnobotanique des plantes aquatiques d'Oued M'Sila (Ksob).", [international] Phytodiversité et Plantes d'intérêt écologique et économique en Algérie Inventaire, Conservation et Valorisation. 29 et 30 octobre 2017. , M'Sila

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