M. SAMARI Houssem

MCA

Directory of teachers

Department

Veterinaire

Research Interests

Parasitology Immunology

Contact Info

University of M'Sila, Algeria

Email : houssem.samari@univ-msila.dz

On the Web:

  • Google Scholar N/A
  • ResearchGate
    ResearchGate N/A
  • ORCID N/A
  • SC
    Scopus N/A

Recent Publications

2025-02-10

First Molecular Detection of Toxoplasma gondii DNA in Blood and Milk of Goats from Algeria

Toxoplasmosis is one of the most important foodborne diseases in humans, potentially acquired by ingesting unpasteurized goat milk. This study examined the role of goat milk as a source of infection of Toxoplasma gondii for humans in Algeria. Sera, blood, and milk samples collected from 106 female goats were tested for the presence of antibodies against T. gondii and its DNA, using indirect ELISA and PCR, respectively. Multiplex PCR was performed using 15 microsatellite markers to determine the clonal type of the T. gondii DNA detected. Seropositive results were found in 51 she-goats (48.11%). T. gondii DNA was detected in 16 (15.09%) and 15 (14.15%) blood and milk samples, respectively. In total, 15 (29.41%) out of 51-seropositive goats were PCR-positive for blood
Citation

M. SAMARI Houssem, (2025-02-10), "First Molecular Detection of Toxoplasma gondii DNA in Blood and Milk of Goats from Algeria", [national] Pathogens , MDPI

Read Full Paper
2024-10-18

Detection of antibiotic residues by a novel rapid test in cow’s milk from north Algeria

Veterinary treatments, particularly antibiotics used for therapeutic or prophylactic purposes in dairy cattle, can be the cause of antibiotic residues in raw milk. The aim of this study is to investigate the use of antibiotics in the dairy cattle industry and to detect the presence of antibiotic residues in raw milk at collection centers using the SNAPduo ST Plus Test in the Bouira and Bordj Bou Arreridj (BBA) regions of Algeria. The results of the survey of 114 breeders showed that the Montbeliarde and Holstein breeds are the most dominant in the farms studied, the frequency of veterinary interventions in cattle farming is always, the curative veterinary intervention represents the highest rate and the beta-lactam is the most family used with 46.25% in Bouira and 85% in BBA, then the oxytetracycline was 22.5% and 15% respectively. Regarding the use of antibiotics by breeders, the majority of them use antibiotics for their animals, with 76.25% in the region of Bouira and 91% in the province of BBA. The antibiotic test of 122 samples of raw milk showed that 19.67% (24/122) of the samples were positive for antibiotic residues with the beta-lactam showing the highest value (79.16%) followed by tetracycline residues with 20.83%. This study showed the presence of antibiotic residues in raw milk in the study area, which may affect the health of dairy consumers.
Citation

M. SAMARI Houssem, Karima Benfodil, Amine Abdeli, Nadir Kaci, Dhaouia Ghida, Dallal Hamichi, Ikram Mahfoud, Sabrina Kerfouf, Cylia Kechad, Amel Hammami, , (2024-10-18), "Detection of antibiotic residues by a novel rapid test in cow’s milk from north Algeria", [national] Brazilian Journal of Animal and Environmental Research , Curitiba

Read Full Paper
2024-10-01

Seroprevalence of Theileria Ovis in Goats from M’sila Region, Central of Algeria

BACKGROUND: In Algeria, data on the epidemiology of theileriosis in small ruminants are
limited.
OBJECTIVES: The current study aims is investigating on the seroprevalence of Theileria spp.
in goats from M’sila region, Central of Algeria.
METHODS: Blood samples of 128 goats from 19 farms were collected from the locality of
Maâdid. The indirect fluorescence antibody test (IFAT) was performed in order to test the sera of
goats for antibodies against Theileria ovis and Theileria lestoquardi.
RESULTS: Out of 128 tested samples, 21 sera (16.40%) were positive for T. ovis antibodies. All
samples were seronegative for T. lestoquardi. The seroprevalence of T. ovis varied from 10% to
30% in the farms. The seropositivity rates did not vary significantly with age, sex, or breeding
system of goats. Tick infested goats were significantly more seropositive than no infested goats.
CONCLUSIONS: The present study reports important data on the epidemiology of caprine
theileriosis from Central of Algeria.
Keywords: IFA test, sera, risk factors, goats, Theileria spp.
Citation

M. SAMARI Houssem, Reghaissia Nassiba, Laadjal Soumia, Ghalmi Farida, Dahmane Abdeldjalil, Hafsi Fella, Cantekin Zafer, Laatamna AbdElkarim, , (2024-10-01), "Seroprevalence of Theileria Ovis in Goats from M’sila Region, Central of Algeria", [national] Iranian Journal of Veterinary Medicine , The University of Tehran's Scientific Journals Database

Read Full Paper
2024-03-13

Seroprevalence Assessment and Risk Factor Analysis of Toxoplasma gondii Infection in Goats from Northeastern Algeria

T. gondii is the causal agent of toxoplasmosis, a worldwide zoonotic disease relevant in
human and veterinary medicine. In Algeria, few reports focused on the presence and circulation
of this parasite in the local goat population. The aim of the survey was to evaluate toxoplasmosis
seroprevalence and associated risk factors. Sera from 460 goats reared on 72 farms in northeastern
Algeria were collected and tested for IgG antibodies to T. gondii by an indirect ELISA. To identify
risk factors, a linear regression analysis of the variables was performed. Anti-T. gondii antibodies
were found in 94.44% (68/72; 95% CI: 73.34–119.73) of goat farms and in 53.26% (245/460; 95%
CI: 46.80–60.36) at the individual level. The multivariable analysis showed that seasonal pasture
(OR =3.804; 95% CI: 3.321–4.358; p = 0.003), presence of water source in pasture area (OR = 4.844; 95%
CI: 1.942–7.789; p = 0.0004), use of anthelminthics (OR = 2.640; 95% CI: 1.592–3.146; p = 0.036), number
of cats, hygiene, proportion of abortions, number of abortions in the last year, year of sampling,
region, and season were the variables significantly associated with T. gondii seropositivity. Abortions
in goat herds seem to be related to T. gondii exposure, thus it is crucial to undertake measures and
strategies to reduce, control, and prevent toxoplasmosis infection in goats, and thereby in humans,
from Algeria.
Citation

M. SAMARI Houssem, Abdeldjalil Dahmane, Daniela Almeida, Nassiba Reghaissia, Djamel Baroudi, Amine Abdelli, AbdElkarim Laatamna, JoãoR.Mesquita, , (2024-03-13), "Seroprevalence Assessment and Risk Factor Analysis of Toxoplasma gondii Infection in Goats from Northeastern Algeria", [national] Animals , MDPI

Read Full Paper
2023-12-15

Occurrence of hydatid cysts in Camelus dromedarius at the slaughterhouse of Tindouf, Southern Algeria

Cystic echinococcosis is a neglected zoonotic parasitic disease of worldwide occurrence, caused by the metacestodes of Echinococcus granulosus sensu lato. In Algeria, cystic echinococcosis has a highest impact on public health and livestock production. Sheep-dog cycle has been described as the main cause of human contamination, but the role of other intermediate hosts such as infested camels is poorly studied. The present survey was conducted to estimate the infection rate of hydatid cyst in dromedary camels at the slaughterhouse of Tindouf, Southern Algeria. From January 2017 to January 2020, a total of 15772 carcasses were examined for cysts detection through gross examination, palpation, and incision of internal organs. Overall, 31 camels (0.20%) were found to be infested. Infection rate was 0.13% in males (19/14660) and 0.27% in females (3/1103). Regarding the age of camels, 10/5047 aged of 5 to 7 years and 12/10725 over 7 years showed an infection rate of 0.20% and 0.11% respectively. Sex and age of 9 camels were not recorded during the present study. The most frequent localization of cystic lesions was in liver (26/31; 87.10%), lung (3/31; 9.69%), and finally in both lung and liver (mixed infection) (2/31; 6.45%). Microscopic examination of the liquid of all hydatid cysts showed a fertility rate of 6.38% (3/47). All fertile cysts were recorded in liver (3/38; 7.89%). This epidemiological study provide data on the importance of cystic echinococcosis in dromedary camels from Algeria, and the role of camels in the epidemiological cycle of E. granulosus sensu lato.
Keywords: Algeria, dromedary camel, fertility, hydatid cyst, prevalence.
Citation

M. SAMARI Houssem, Nassiba Reghaissia, Abdeldjalil Dahmane, , (2023-12-15), "Occurrence of hydatid cysts in Camelus dromedarius at the slaughterhouse of Tindouf, Southern Algeria", [national] Journal of Camelid Science , ISOCARD

Read Full Paper
2023-10-24

Contribution à l’étude épidémiologique des infections à Cryptosporidium Spp. chez les poissons d’eau douce dans la wilaya de Guelma

La présente étude s’est donnée comme objectif principal d’apporter une contribution à l’étude épidémiologique des infections à Cryptosporidium Spp chez le poisson. Pour cela, nous avons collecté 37 poissons d’eau douce à partir du Barrage de Bouhamdane (Situé sur la wilaya de Guelma), durant la période qui s’étale entre Décembre 2021 et Juillet 2022. Au total, 61 prélèvements (37grattage intestinal, 24 matière fécale) ont été collectés a partir de 03 espèces de poissons, Carassius carassius (11/37), Abramis brama (09/37) et Barbus barbus (17/37) qui sont appelées communément carassin commun, brème commun et barbeau commun respectivement. L’analyse des prélèvements a été effectuée en utilisant la coloration de Ziehl Neelsen modifiée après les confections des frottis sur les lames à microscope optique afin de rechercher les oocystes de Cryptosporidium Spp. sous microscope optique. La prévalence totale était de 40.54% (15/37). L’analyse statistique des facteurs de risque a démontré que les infections sont supérieures chez l’espèce Barbus barbus (54.05%) en période chaude de l’année (54.05%) tandis que la taille des poissons n’a donnée aucune déférence significative (P>0.05). Le test de McNemar est également, nous a donné qu’il y’a plus des chances de détecter les oocystes dans le grattage intestinal par rapport à la matière fécale avec une différence de 8.33%.
Mots clé: Barrage De Bouhamdane. Cryptosporidium Spp, Oocyste, Poisson, Prévalence.
Citation

M. SAMARI Houssem, Nassiba Reghaissia, Souaki Mohamed Atif, , (2023-10-24), "Contribution à l’étude épidémiologique des infections à Cryptosporidium Spp. chez les poissons d’eau douce dans la wilaya de Guelma", [international] 4th International Day of the Research Laboratory HASAQ "Cryptosporidium spp., Giardia duodenalis and Veterinary Public Health" , ENSV d'Alger

Read Full Paper
2023-10-14

First Epidemiological Report on the Prevalence and Associated Risk Factors of Cryptosporidium spp. in Farmed Marine and Wild Freshwater Fish in Central and Eastern of Algeria

The present study aimed to estimate the prevalence and molecular characterization of Cryptosporidium spp. in six different fish species both from marine and freshwater environments.
During a period of 2 years (2018–2020), a total of 415 fecal samples and 565 intestinal scrapings were collected in seven provinces from the central and eastern Algeria. From those, 860 fish belonged to six different species, two of which are cultured marine and four are wild freshwater fish. All samples were screened for Cryptosporidium spp. presence using molecular techniques. Nested PCR approach was performed to amplify partial sequences of the small subunit ribosomal RNA (SSU rRNA) and 60-kDa glycoprotein (GP60) genes for Cryptosporidium genotyping and subtyping. Detailed statistical analysis was performed to assess the prevalence variation of Cryptosporidium infection according to different risk factors. Nested PCR analysis of SSU gene revealed 173 Cryptosporidium positive fish, giving an overall prevalence of 20.11% (17.5–23.0). Cryptosporidium spp. was detected in 8.93% (42/470) of cultured marine fish and 33.58% (131/390) of wild freshwater fish. Overall, the prevalence was affected by all studied risk factors, except the gender. Molecular characterization and subtyping of Cryptosporidium isolates showed occurrence of IIaA16G2R1 and IIaA17G2R1 subtypes of C. parvum in the fish species Sparus aurata. The present study provides the first epidemiological data on the prevalence and associated risk factors of Cryptosporidium spp. in farmed marine and wild freshwater fish and the first molecular data on the occurrence of zoonotic C. parvum in fish from North Africa (Algeria).
Citation

M. SAMARI Houssem, Nassiba Reghaissia, Sadiya Maxamhud, AbdElkarim Laatamna, AbdEldjalil Dahmane, Rabeh Berima, Amine Abdelli, Ahcene Hakem, Djamel Baroudi, Anastasios D. Tsaousis, , (2023-10-14), "First Epidemiological Report on the Prevalence and Associated Risk Factors of Cryptosporidium spp. in Farmed Marine and Wild Freshwater Fish in Central and Eastern of Algeria", [international] 4th International Day of the Research Laboratory HASAQ "Cryptosporidium spp., Giardia duodenalis and Veterinary Public Health" , ENSV d'Alger

Read Full Paper

Molecular Identification of Cryptosporidium spp., and Giardia duodenalis in Dromedary Camels (Camelus dromedarius) from the Algerian Sahara

Intestinal microbial parasites are major contributors to the global burden of gastrointestinal disease. Such infections are mainly caused by Cryptosporidium, Giardia duodenalis, and Entamoeba histolytica. These parasites are transmitted either directly or indirectly through oral–fecal routes. Previous reports suggested that camels could play a role in the zoonotic transmission of various clinically and veterinary important intestinal parasites, however, limited data are available on intestinal infections of camels, particularly on a molecular level. We aimed to explore the occurrence of these three parasites in camels (Camelus dromedarius) in Algeria. (2) A total of 68 samples—63 stool samples from camels and five from the environment—were collected from two desert regions in Algeria and analyzed using PCR and qPCR methods. (3) Overall, 7% of the camels tested positive for zoonotic subtypes of Cryptosporidium spp., while 16% of the camels tested positive for G. duodenalis. Two environmental samples also tested positive for G. duodenalis. None of the samples were positive for Entamoeba histolytica. (4) Our results provide one of the first molecular-based identification of these gut parasites in dromedary camels in Algeria. The presence of G. duodenalis in the host and the environment unveils, in part, the circulation route of this parasite. Our results will spearhead further investigations into the prevalence and epidemiology of gut parasites in hoofed animals and raise questions concerning their role in health and disease in the area.
Keywords: Camelus dromedarius; Cryptosporidium spp.; Giardia duodenalis; Entamoeba histolytica;
genotyping; prevalence; Algeria
Citation

M. SAMARI Houssem, Nassiba Reghaissia, Sadiya Maxamhud, AbdElkarim Laatamna, Nacira Remdani, Eleni Gentekaki, Anastasios D. Tsaousis, , (2023-10-14), "Molecular Identification of Cryptosporidium spp., and Giardia duodenalis in Dromedary Camels (Camelus dromedarius) from the Algerian Sahara", [international] 4th International Day of the Research Laboratory HASAQ "Cryptosporidium spp., Giardia duodenalis and Veterinary Public Health" , ENSV d'Alger

Read Full Paper
2022

INFUENCE DES FACTEURS ZOOTECHNIQUES ET SANITAIRES SUR LA CROISSANCE DES POULETTES DEMARREES ET LA PRODUCTION DES ŒUFS DE CONSOMMATION CHEZ LA POULE PONDEUSE

L’élevage de la poule pondeuse représente une activité importante dans la production animale, mais elle est influencée par différente paramètres que l’éleveur doit maitriser.
Le présent travail est effectué dans le but de l’évaluation de l’influence des facteurs zootechniques et sanitaires sur la croissance des poulettes démarrées ainsi que le niveau de la production des œufs de consommation chez la poule pondeuse dans deux unités différentes et établir une comparaison entre nous résultat avec les normes référencie comme les guides d’élevages.
Dans ce sens, les deux unités ont été visitées hebdomadairement durant une période de 10 mois (43 semaines).
Notre étude sur les unités révèle une conduite d’élevage défaillante suite au non-respect des normes de l’ensemble des paramètres recommandé. On a noté des conditions d’ambiances non contrôlées, une mauvaise conduite alimentaire et un rationnement inadéquat avec gaspillage important d’aliment (augmentation de l’indice de: consommation et conversion) et un suivi sanitaire inopérant. Ceci s’est traduit par des taux des mortalités élevées et une baisse des performances zootechniques du cheptel.
Mots clefs : poule pondeuse, poulette démarrée, bâtiment, paramètre zootechnique et sanitaire.
Citation

M. SAMARI Houssem, Reghaissia Nassiba, Dahman Abdeldjalil, Laatamna Abdelkarim, , (2022), "INFUENCE DES FACTEURS ZOOTECHNIQUES ET SANITAIRES SUR LA CROISSANCE DES POULETTES DEMARREES ET LA PRODUCTION DES ŒUFS DE CONSOMMATION CHEZ LA POULE PONDEUSE", [national] 1er Séminaire national sur la Zootechnie , Souk ahras

Read Full Paper

Séroprévalence et facteurs de risques des infections à Brucella abortus chez les ovins dans la région de Sétif

La brucellose est une zoonose majeure qui se caractérise par l'avortement et la baisse de la fertilité chez de nombreuses espèces.
Dans notre étude, nous nous sommes intéressés à la région de Sétif. Nos prélèvements ont été effectués en 2016.
Notre étude a porté sur l'évaluation de la séroprévalence et l’analyse de quelques facteurs de risque de la brucellose ovine. Elle a porté sur un échantillon de 129 prélèvements ovins provenant de 15 élevages de la région étudiée.
Les résultats globaux obtenus, montrent une séroprévalence d’élevage de 33.3 % et de 66.6%, et une séroprévalence individuelle apparente de 11.62% et 13.95% ont été constatées respectivement par l’épreuve au Rose Bengale et le test de Wright.
Cette prévalence varie en fonction de certains facteurs de risque tel que la région, le sexe, l'âge, la gestation, la présence d’avortement et au type d'élevage (mixte et non mixte).
Mots clés : Brucellose, ovin, Séroprévalence, Rose Bengale, Wright.
Citation

M. SAMARI Houssem, Reghaissia Nassiba, Dahman Abdeldjalil, Laatamna Abdelkarim, , (2022), "Séroprévalence et facteurs de risques des infections à Brucella abortus chez les ovins dans la région de Sétif", [national] 1er Séminaire national sur la Zootechnie , Souk ahras

Read Full Paper

L’infection à Toxoplasma gondii chez le caprin dans la région de Mila ; prévalence et impact sur la santé publique

La connaissance de la prévalence et des facteurs de risque associés d’une infection
par T. gondii est nécessaire pour une bonne compréhension de son épidémiologie,
ainsi que leurs implications en termes des stratégies du contrôle adaptées aux
conditions locales.
Une étude a été réalisée dans la région de Mila pour déterminer la prévalence de T.
gondii, et les facteurs de risque associés, à partir de la viande caprine destinée à la
consommation humaine. Entre Janvier et Juin 2022, 80 échantillons de cœur du caprin
ont été prélevés des abattoirs et soumis à l’analyse histopathologique après la fixation
au formol 10%. Cette analyse a révélé un taux de d’infection de 51.25% (41/80) suite
à la présence des kystes à bradyzoites de Toxoplasma gondii.
L’analyse de certains facteurs supposés être en rapport avec l’infection par T. gondii
(à savoir l’âge, le sexe, le type d’élevage et la présence de chat) n’a montré aucun lien
significatif entre ces facteurs et l’infection par ce parasite (P>0.05). La haute prévalence
observée peut être due à plusieurs facteurs en association induisant ainsi une forte
contamination de l’environnement ; tels la densité de chats domestiques dans la région,
la résistance des oocystes excrétés par ces chats dans la nature en forte association
avec les condition climatiques favorables à la sporulation et la survie, assurant une
longue période de viabilité de cette forme infectieuse, et d’autre part, le contact répété
et long des caprins avec le parasite (en rapport avec le mode de pâturage fréquent).
Ces résultats préliminaires suggèrent un risque accru d’infection à T. gondii chez
l’homme suite à la consommation de viandes insuffisamment cuites ou du lait cru des
chèvres, pour éviter un tel risque, la viande doit être cuite à une température interne
supérieure à 70 °C, pour détruire les kystes à bradyzoites de T. gondii éventuellement
présents.
KEYWORDS: Caprin, Toxoplasma gondii, Histopathologie, Santé publique, Mila
Citation

M. SAMARI Houssem, Dahman Abdeldjalil, Reghaissia Nassiba, Laatamna Abdelkarim, , (2022), "L’infection à Toxoplasma gondii chez le caprin dans la région de Mila ; prévalence et impact sur la santé publique", [international] 1st International Seminar on Veterinary Parasitology , El Taref

Read Full Paper

Séroprévalence, facteurs de risques et identification des tiques dans le cas des infections à Anaplasma spp chez les caprins dans le nord-Est algérien

L’anaplasmose est une maladie vectorielle d’origine bactérienne transmise par les
tiques. Elle est considérée comme une infection zoonotique émergente à impact
économique et épidémiologique important dans le monde. En Algérie, certaines études
ont été décrites concernant l’anaplasmose bovine, ovine et canine mais aucune étude
n’a été réalisée pour la recherche d’une éventuelle circulation d’Anaplasma spp chez
le caprin. De ce fait, il nous a paru intéressant de réaliser une enquête épidémiologique
sur les pathogènes érythrocytaires d’Anaplasma. Le présent travail s’est donné comme
objectif principal d’apporter une contribution à la connaissance des infections par
Anaplasma spp chez le caprin dans la région Nord-Est d’Algérie (wilaya de Guelma et
wilaya d’El-taref), et les facteurs de risque associés. Ainsi d’étudier morphologiquement
le vecteur prélevé dans cette étude. Pour cela, 182 prélèvements sanguins de caprin
et 102 prélèvements des tiques, ont été effectués entre mars et septembre 2016 puis
analysés par ELISA. Des informations relatives aux caractéristiques individuelles des
animaux ainsi qu’aux pratiques d’élevage ont été recueillies. La séroprévalence globale
par cELISA pour Anaplasma spp. a été de 78.02%. L’analyse des facteurs de risque,
susceptibles d’influencer la prévalence de l’agent pathogène étudiés, a montré
l’influence de l’état de gestation (P=0.002), de la saison (P=0.015), les conditions
climatiques (p=0.000) et le type d’élevage (p=0.000). Parmi les 102 tiques récoltées
sur un total de 55 caprins, deux espèces ont été identifiées Rhipicephalis sanguineus
et Rhipicephalis bursa, avec une nette prédominance de Rhipicephalis sanguineus
(85.39%).

KEYWORDS: Anaplasma spp, cELISA, facteurs de risque, Caprin, Tique, Nord-Est d’Algerie.
Citation

M. SAMARI Houssem, Reghaissia Nassiba, Dahman Abdeldjalil, Laatamna Abdelkarim, Ghalmi Farida, Azzag Naouel, , (2022), "Séroprévalence, facteurs de risques et identification des tiques dans le cas des infections à Anaplasma spp chez les caprins dans le nord-Est algérien", [international] the first International Seminar on Veterinary Parasitology. , El Taref

Read Full Paper

Molecular characterization of Echinococcus granulosus sensu lato genotypes in dromedary camels from extreme Sahara of Algeria based on analysis of nad2 and nad5 genetic markers

Cystic echinococcosis is parasitic disease caused by the metacestodes belonging to the Echinococcus granulosus sensu lato (s.l.) species complex. Cystic echinococcosis is of considerable economic and public health importance. It is endemic in both livestock and humans in North African countries, including Algeria. The present study aimed to characterize E. granulosus s.l. genotypes in dromedary camels (Camelus dromedarius) from the extreme Sahara of Algeria, using recently developed mitochondrial genetic markers (NADH dehydrogenase subunit 2 and NADH dehydrogenase subunit 5) for reliable identification of different genotypes. A total of 75 Echinococcus cysts were collected from 49 dromedary camels, including 65 and 10 cysts from 45 and four camels originating from two slaughterhouses of Tindouf and Illizi provinces, respectively. E. granulosus sensu stricto (s.s.) G1 and G3 were identified in camels from both areas based on nad5 (649 bp) gene sequences, whereas E. granulosus s.l. G6 was identified in camels from Tindouf region based on concatenated nad5 and nad2 gene sequences (total 1336 bp). Identified samples clustered into 11 different haplotypes (ALG1-ALG11), including four haplotypes (ALG8-ALG11) for E. granulosus s.s. G1, one haplotype (ALG7) for E. granulosus s.s. G3, and six haplotypes (ALG1-ALG6) for E. granulosus s.l. G6. The present study provides valuable molecular data, including genotyping and haplotypic variability, on E. granulosus s.l. in dromedary camels from two regions in the extreme Sahara of Algeria. Future characterization of the G1, G3, and G6 samples based on sequencing of complete mitochondrial genomes would be of considerable significance for a more comprehensive understanding of molecular epidemiology of CE in Algeria.
Citation

M. SAMARI Houssem, Teivi Laurimäe, Nassiba Reghaissia, AbdEldjalil Dahmane, Ali Mamoune, Djamel Baroudi, Peter Deplazes, AbdElkarim Laatamna, , (2022), "Molecular characterization of Echinococcus granulosus sensu lato genotypes in dromedary camels from extreme Sahara of Algeria based on analysis of nad2 and nad5 genetic markers", [national] Acta tropica , ELSEVIER

Read Full Paper
2021

First report on occurrence of zoonotic helminth Toxocara canis, Toxascaris leonina and Ancylostoma caninum in domestic dogs from province of Djelfa, Algeria

A total of 85 faecal samples of domestic dogs were collected from six localities, including Birine, Sidi
Ladjel, Dar Chioukh, Aïn Maâbed, Aïn El-Ibel and Djelfa city from province of Djelfa, Algeria. Samples were tested
for presence of Toxocara canis, Toxascaris leonina and Ancylostoma caninum eggs by formol-ether concentration and
flotation techniques. Microscopic examination revealed an infection rate of 9.4%, 15.3% and 1.15% for T. canis, T.
leonina and A. caninum, respectively. Males were more frequently infected with T. leonina as compared to females,
while, no difference was observed regarding T. canis and A. caninum. Prevalence of T. canis and T. leonina did not vary
significantly with age ranges of dogs. In contrast, A. caninum seems to be more found in dogs aged of 6–12 months.
Prevalence of the three species did not vary significantly in dogs without diarrhea and those with diarrhea. Through
these preliminary findings, dogs from steppic region of Djelfa can play a potential role in the dissemination of these
neglected zoonotic helminths, which may endanger health of peoples, particularly, pastoral community.
Citation

M. SAMARI Houssem, AbdElkarim LAATAMNA, Djamel BAROUDI, Hanane ZIANE, Oumenoune ALIM, Meriem TELIBI, Djemaa TAOUSSI, , (2021), "First report on occurrence of zoonotic helminth Toxocara canis, Toxascaris leonina and Ancylostoma caninum in domestic dogs from province of Djelfa, Algeria", [national] Annals of Parasitology , Polish Parasitological Society

Read Full Paper
2019

Prevalence of Cryptosporidium spp. and Eimeria spp. in lambs from steppe region of Djelfa, Algeria

Genus of Cryptosporidium and Eimeria are apicomplexan parasites infecting the digestive
tract of wide range of mammals, birds and fish. Eimeria species are recognized by their
strict host specificity, while, Cryptosporidium spp. crosses the species barrier. Both
parasites cause considerable economic impact on the livestock industry (mainly in
ruminants). Cryptosporidium parvum was considered as the common zoonotic pathogen
causing diarrhea and other gastrointestinal disorders in ruminants as well as human. In
Algeria, compared to other countries, a little data are available on the epidemiology of
animal cryptosporidiosis (prevalence, associated risk factors and molecular investigation),
where, a low number of studies have been reported in cattle, birds, horse and sheep. The
purpose of this study was to investigate the prevalence of Cryptosporidium spp. and
Eimeria spp. and associated potential risk factors in some lamb’s farms from steppe
region of Djelfa (Algeria). Therefore, a total of 170 fresh fecal samples were subjected to
macroscopic examination and were processed for microscopic examination of Eimeria
oocysts after concentration by formalin-ether sedementation. Microscopic detection of
Cryptosporidium spp. was performed by modified Ziehl-Neelsen staining. Infection rate
of Cryptosporidium spp. and Eimeria spp. was seen in 26.5% (45/170) and 48.8%
(83/170) of examined lambs respectively. The mixed infection was recorded in 16.5%
(28/170) of screened lambs. Occurrence of infection by both parasites was slightly higher
in female than male lambs. Age of lambs seem to be involved in Cryptosporidium
prevalence in contrary to Eimeria. In addition, there was no association between
Cryptosporidium and Eimeria infection’s and the consistency of the stool samples
(presence or absence of diarrhea). Future exhaustive studies covering an important
number of provinces of the country should be carried out to better understand the
epidemiology of these parasitic diseases and evaluate their economic impact on sheep
farming in Algeria.
Keywords: Cryptosporidium spp., Eimeria spp., prevalence, lambs, Djelfa
Citation

M. SAMARI Houssem, BOURAGBA Messaoud, LAATAMNA AbdElkarim, HEBALI Samiha, ZENATI Saida, HAKAM Ahcene, BAROUDI Djamel, , (2019), "Prevalence of Cryptosporidium spp. and Eimeria spp. in lambs from steppe region of Djelfa, Algeria", [international] La 14ème journée Internationales des Sciences Vétérinaires sous le thème Pathologie Infectieuses Animales , Alger

Read Full Paper

Prevalence of Cryptosporidium spp. in farmed animals from steppe and highplateau regions in Algeria

This study was conducted to investigate the prevalence of Cryptosporidium spp.and associated potential risk factors in farmed animals from different steppe and high plateauregions in Algeria. A total of 289, 254 faecal samples of cattle, sheep respectively, andtracheas of 135 broiler chickens were screened for the presence of Cryptosporidium spp. byformalin-ether concentration method and modified Ziehl-Neelsen staining. Overall,Cryptosporidium spp. was detected in 36.7%, 15%, and 8.9% of examined cattle, sheep andbroiler chickens. In cattle, the highest prevalence was observed in the neonatal calves (52.6%)and the presence of Cryptosporidium spp. was significantly associated with diarrhoea. Ovinecryptosporidiosis was found in more of 80% of sampled farms and lambs aged between 1-6months (20.3%), followed by neonatal lambs (18.7%) were the most infected.Cryptosporidium excretion in sheep was not associated with presence of diarrhoea. Thepresence of cryptosporidia in broiler chickens showed a higher rate in birds aged of 16-24days (30%) than in those of 35-44 days (3.5%). None of broiler chickens more than 44 dayswas found to be positive for Cryptosporidium. The prevalence was not affected by sex in allstudied animal species. The results of the present study provide the first data on theprevalence of respiratory cryptosporidiosis in broiler chickens from steppe and high plateauregions in Algeria
Keywords: Prevalence, Cryptosporidium spp., Farmed animals, Steppe and High plateau regions,Algeria
Citation

M. SAMARI Houssem, Laatamna Abdelkarim, Bouragba Messaoud, , (2019), "Prevalence of Cryptosporidium spp. in farmed animals from steppe and highplateau regions in Algeria", [national] La XXIIème journée nationale de parasitologie te mycologie , Alger

Read Full Paper

La brucellose ovine dans la région Sud-Ouest de Sétif : étude séro-épidémiologique et évaluation de deux tests sérologiques (le Rose Bengale et le Wright).

La brucellose est une zoonose qui se caractérise par l'avortement et la baisse de la fertilité chez de nombreuses espèces.
Dans notre étude, nous nous sommes intéressés à la région Sud-Ouest de la wilaya de Sétif comprenant deux communes. Nos prélèvements ont été effectués en septembre et octobre 2016.
Notre étude a porté sur les caractéristiques du test de Wright par rapport à l’épreuve au Rose Bengale considéré comme Gold standard avec une évaluation de la séroprévalence et l’analyse de quelques facteurs de risque de la brucellose ovine. Elle a porté sur un échantillon de 129 prélèvements ovins provenant de 15 élevages de la région étudiée.
Les résultats globaux obtenus, montrent une concordance faible entre les deux techniques (k=0,37) avec une valeur du test Mc Nemar égale à 0.42et une exactitude de 91.47%. Le test de Wright (Se= 40% et Sp= 89,4%) est donc moins sensible même s’il reste aussi spécifique que l’épreuve du Rose Bengale.
Le test de Wright s’avère avoir une VPP faible (VPP= 33.33%), mais une VPN élevée (VPN= 91.89%). Ces résultats s’expliquent par l’existence d’un nombre important de faux positifs. Une séroprévalence d’élevage de 33.3 % et de 66.6%, et une séroprévalence individuelle apparente de 11.62% et 13.95% ont été constatées respectivement par l’épreuve au Rose Bengale et le test de Wright.
Cette prévalence varie en fonction de certains facteurs de risque tel que la région, le sexe, l'âge, la gestation, la présence d’avortement et au type d'élevage (mixte et non mixte).
Mots clés : Brucellose, ovin, Séroprévalence, Rose Bengale, Wright, sensibilité et spécificité.
Citation

M. SAMARI Houssem, Messai chafik reda, Menadi salah, Reghaissia nassiba, Ghalmi farida, Hafsi fella, , (2019), "La brucellose ovine dans la région Sud-Ouest de Sétif : étude séro-épidémiologique et évaluation de deux tests sérologiques (le Rose Bengale et le Wright).", [international] La 14ème journée Internationales des Sciences Vétérinaires sous le thème Pathologie Infectieuses Animales , ENSV d'Alger

Read Full Paper

Diversity of gastrointestinal parasites in captive and domestic birds from zoological parks and one rural locality of Algeria

Microscopic examination of 84 fresh fecal samples of 11 different species of birds from three zoological parks and one rural locality from Algiers and the Setif province (central-eastern of Algeria) indicated an overall prevalence of gastro-intestinal parasites 19% (16/84). Overall, genera of Cryptosporidium Tyzzer, 1907, Eimeria Schneider, 1875, Capillaria Zeder, 1800, and larvae of strongyles type were recorded with prevalence of 6%, 4.8%, 3.6%, and 4.8% respectively. Protozoa of Cryptosporidium was found only in ostrich (31.2%), whereas, Eimeria spp. was observed in pigeons (40%), hens (20%) and ring-necked pheasants (14.3%). Larvae of strongyles were recorded in mallards (11.1%), emus (14.3%) and peacocks (9.1%), whereas, eggs of Capillaria was detected only in peacocks (27.3%). No mixed infection was recorded in infected birds. Parasite fauna infecting the digestive tract of birds from Algerian zoological parks is a little diversified. Further studies should be carried out to better show the diversity of gastro-intestinal parasites of wild and domestic birds in Algeria across exhaustive studies covering many regions.
Citation

M. SAMARI Houssem, Abdelkarim Laatamna, Yacine Chebhi, Lotfi Betal, , (2019), "Diversity of gastrointestinal parasites in captive and domestic birds from zoological parks and one rural locality of Algeria", [national] Biodiversity Journal , Edizioni Danaus

Read Full Paper
← Back to Researchers List